Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

• Home Page
• Cancer-specific
• Epitope Search
• Data Retrieval
• Browse Gene
• Browse Tissue
• Sequence Similarity
• Partially Personalized
• Submit a Protein
• Vaccine Target
• Proteome Data
• NGS data
• BLAST & Predict
• Fully Personalized
• Proteins Pair
• Proteome Pair
• Advanced Tools
• Epitope Mapping
• Cross Reactivity
• Important Information
• Acknowledgement
• Algorithm
• Help
• Developers
• Contact

Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_008835                                                              20     390
NP_001075109                                                           20     390
NP_006112                                                              17    4312
NP_060420                                                              16    7355
NP_001230271                                                           16    7355
NP_005952                                                              16    7355
XP_006725823                                                           16    7355
XP_006725595                                                           16    7355
XP_005257548                                                           16    7355
XP_006710300                                                           16    7355
NP_005742                                                              15    9605
NP_671714                                                              15    9605
NP_003310                                                              15    9605
NP_597676                                                              15    9605
NP_001243779                                                           15    9605
NP_596869                                                              15    9605
NP_001272829                                                           15    9605
NP_056347                                                              15    9605
XP_006724942                                                           15    9605
XP_005256248                                                           15    9605
XP_005250160                                                           15    9605
XP_005250159                                                           15    9605
XP_005246888                                                           15    9605
XP_006712788                                                           15    9605
XP_006712787                                                           15    9605
XP_006712786                                                           15    9605
XP_006710331                                                           15    9605
NP_542938                                                              15    9605
NP_113678                                                              15    9605
NP_001254479                                                           15    9605
NP_597681                                                              15    9605

>NP_008835
          Length = 4128

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    MRSSVDNTK 9
            M+SSVDNTK
Sbjct: 1583 MQSSVDNTK 1591



>NP_001075109
          Length = 4097

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    MRSSVDNTK 9
            M+SSVDNTK
Sbjct: 1583 MQSSVDNTK 1591



>NP_006112
          Length = 644

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   MRSSVDNTK 9
           +RS +DN K
Sbjct: 408 LRSEIDNVK 416



>NP_060420
          Length = 1142

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   MRSSVDN 7
           MRSSVD+
Sbjct: 920 MRSSVDS 926



>NP_001230271
          Length = 1030

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   MRSSVDN 7
           MRSSVD+
Sbjct: 920 MRSSVDS 926



>NP_005952
          Length = 2439

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2   RSSVDN 7
           RSSVDN
Sbjct: 654 RSSVDN 659



>XP_006725823
          Length = 1825

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2   RSSVDN 7
           RSSVDN
Sbjct: 654 RSSVDN 659



>XP_006725595
          Length = 1311

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2   RSSVDN 7
           RSSVDN
Sbjct: 654 RSSVDN 659



>XP_005257548
          Length = 749

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   MRSSVDN 7
           MRSSVD+
Sbjct: 527 MRSSVDS 533



>XP_006710300
          Length = 1116

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   MRSSVDNTK 9
           + SSVD TK
Sbjct: 980 LSSSVDTTK 988



>NP_005742
          Length = 3907

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2    RSSVDN 7
            RSS+DN
Sbjct: 1640 RSSIDN 1645



>NP_671714
          Length = 3899

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2    RSSVDN 7
            RSS+DN
Sbjct: 1640 RSSIDN 1645



>NP_003310
          Length = 26926

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2    RSSVDNTK 9
            R S+DN+K
Sbjct: 8256 RLSIDNSK 8263



>NP_597676
          Length = 27051

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2    RSSVDNTK 9
            R S+DN+K
Sbjct: 8381 RLSIDNSK 8388



>NP_001243779
          Length = 34350

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2     RSSVDNTK 9
             R S+DN+K
Sbjct: 15680 RLSIDNSK 15687



>NP_596869
          Length = 33423

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2     RSSVDNTK 9
             R S+DN+K
Sbjct: 14753 RLSIDNSK 14760



>NP_001272829
          Length = 765

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   SSVDNTK 9
           SSVDN K
Sbjct: 513 SSVDNIK 519



>NP_056347
          Length = 368

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   SSVDNTK 9
           SSVDN K
Sbjct: 116 SSVDNIK 122



>XP_006724942
          Length = 641

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2   RSSVDNT 8
           RSSVD T
Sbjct: 211 RSSVDTT 217



>XP_005256248
          Length = 808

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1  MRSSVDNTK 9
          MR+SVD+ +
Sbjct: 29 MRTSVDSAR 37



>XP_005250160
          Length = 3939

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2    RSSVDN 7
            RSS+DN
Sbjct: 1640 RSSIDN 1645



>XP_005250159
          Length = 3952

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2    RSSVDN 7
            RSS+DN
Sbjct: 1640 RSSIDN 1645



>XP_005246888
          Length = 22663

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2    RSSVDNTK 9
            R S+DN+K
Sbjct: 3993 RLSIDNSK 4000



>XP_006712788
          Length = 24022

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2    RSSVDNTK 9
            R S+DN+K
Sbjct: 5352 RLSIDNSK 5359



>XP_006712787
          Length = 33978

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2     RSSVDNTK 9
             R S+DN+K
Sbjct: 15308 RLSIDNSK 15315



>XP_006712786
          Length = 35484

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2     RSSVDNTK 9
             R S+DN+K
Sbjct: 16814 RLSIDNSK 16821



>XP_006710331
          Length = 641

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2   RSSVDNT 8
           RSSVD T
Sbjct: 211 RSSVDTT 217



>NP_542938
          Length = 560

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1   MRSSVDNT 8
           M SSVD T
Sbjct: 373 MNSSVDET 380



>NP_113678
          Length = 852

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1  MRSSVDNTK 9
          MR+SVD+ +
Sbjct: 29 MRTSVDSAR 37



>NP_001254479
          Length = 35991

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2     RSSVDNTK 9
             R S+DN+K
Sbjct: 17321 RLSIDNSK 17328



>NP_597681
          Length = 27118

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2    RSSVDNTK 9
            R S+DN+K
Sbjct: 8448 RLSIDNSK 8455



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.300    0.108    0.261 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 444,552
Number of extensions: 591
Number of successful extensions: 31
Number of sequences better than 10000.0: 31
Number of HSP's gapped: 31
Number of HSP's successfully gapped: 31
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 17 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.3 bits)
S2: 28 (15.4 bits)