Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

• Home Page
• Cancer-specific
• Epitope Search
• Data Retrieval
• Browse Gene
• Browse Tissue
• Sequence Similarity
• Partially Personalized
• Submit a Protein
• Vaccine Target
• Proteome Data
• NGS data
• BLAST & Predict
• Fully Personalized
• Proteins Pair
• Proteome Pair
• Advanced Tools
• Epitope Mapping
• Cross Reactivity
• Important Information
• Acknowledgement
• Algorithm
• Help
• Developers
• Contact

Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_004963                                                              20     390
XP_005251512                                                           20     390
NP_071504                                                              17    3301
NP_000911                                                              17    3301
NP_001035806                                                           17    3301
XP_006718641                                                           17    3301
XP_005274089                                                           17    3301
XP_005274088                                                           17    3301
XP_006718640                                                           17    3301
NP_057576                                                              16    5631
NP_001171272                                                           16    5631
NP_001171273                                                           16    5631
XP_005256675                                                           16    5631
NP_056391                                                              16    7355
XP_005265093                                                           16    7355
XP_006713164                                                           16    7355
XP_005245618                                                           16    7355
NP_003657                                                              16    7355
NP_003879                                                              15    9605
NP_001193826                                                           15    9605
NP_733797                                                              15    9605
NP_733798                                                              15    9605
NP_001265354                                                           15    9605
NP_000680                                                              15    9605
NP_000684                                                              15    9605
NP_001243323                                                           15    9605
NP_000683                                                              15    9605
NP_443124                                                              15    9605
NP_060418                                                              15    9605
XP_006717236                                                           15    9605
XP_006717235                                                           15    9605
XP_006717234                                                           15    9605
XP_006717233                                                           15    9605
XP_006717232                                                           15    9605
XP_006717231                                                           15    9605
XP_006717230                                                           15    9605
XP_006717229                                                           15    9605

>NP_004963
          Length = 1132

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   MRIAKENNQ 9
           MRIAKEN+Q
Sbjct: 187 MRIAKENDQ 195



>XP_005251512
          Length = 1132

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   MRIAKENNQ 9
           MRIAKEN+Q
Sbjct: 187 MRIAKENDQ 195



>NP_071504
          Length = 1178

 Score = 16.9 bits (32), Expect =  3301
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   MRIAKENN 8
           +++AKENN
Sbjct: 103 IKVAKENN 110



>NP_000911
          Length = 1178

 Score = 16.9 bits (32), Expect =  3301
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   MRIAKENN 8
           +++AKENN
Sbjct: 103 IKVAKENN 110



>NP_001035806
          Length = 1178

 Score = 16.9 bits (32), Expect =  3301
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   MRIAKENN 8
           +++AKENN
Sbjct: 103 IKVAKENN 110



>XP_006718641
          Length = 1178

 Score = 16.9 bits (32), Expect =  3301
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   MRIAKENN 8
           +++AKENN
Sbjct: 103 IKVAKENN 110



>XP_005274089
          Length = 1178

 Score = 16.9 bits (32), Expect =  3301
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   MRIAKENN 8
           +++AKENN
Sbjct: 103 IKVAKENN 110



>XP_005274088
          Length = 1178

 Score = 16.9 bits (32), Expect =  3301
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   MRIAKENN 8
           +++AKENN
Sbjct: 103 IKVAKENN 110



>XP_006718640
          Length = 1178

 Score = 16.9 bits (32), Expect =  3301
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   MRIAKENN 8
           +++AKENN
Sbjct: 103 IKVAKENN 110



>NP_057576
          Length = 186

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   RIAKENNQ 9
           +IAKEN Q
Sbjct: 110 QIAKENQQ 117



>NP_001171272
          Length = 146

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   RIAKENNQ 9
           +IAKEN Q
Sbjct: 110 QIAKENQQ 117



>NP_001171273
          Length = 165

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   RIAKENNQ 9
           +IAKEN Q
Sbjct: 110 QIAKENQQ 117



>XP_005256675
          Length = 118

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   RIAKENNQ 9
           +IAKEN Q
Sbjct: 110 QIAKENQQ 117



>NP_056391
          Length = 957

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   MRIAKENNQ 9
           MR++ E NQ
Sbjct: 211 MRVSHEENQ 219



>XP_005265093
          Length = 969

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   MRIAKENNQ 9
           MR++ E NQ
Sbjct: 211 MRVSHEENQ 219



>XP_006713164
          Length = 969

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   MRIAKENNQ 9
           MR++ E NQ
Sbjct: 211 MRVSHEENQ 219



>XP_005245618
          Length = 267

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   RIAKENNQ 9
           R+A+E NQ
Sbjct: 179 RLAREKNQ 186



>NP_003657
          Length = 400

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   RIAKENNQ 9
           R+A+E NQ
Sbjct: 179 RLAREKNQ 186



>NP_003879
          Length = 518

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   MRIAKE 6
           MRIAKE
Sbjct: 411 MRIAKE 416



>NP_001193826
          Length = 497

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   MRIAKE 6
           MRIAKE
Sbjct: 390 MRIAKE 395



>NP_733797
          Length = 480

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   MRIAKE 6
           MRIAKE
Sbjct: 373 MRIAKE 378



>NP_733798
          Length = 422

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   MRIAKE 6
           MRIAKE
Sbjct: 315 MRIAKE 320



>NP_001265354
          Length = 1774

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   RIAKENN 8
           R+AKE N
Sbjct: 936 RVAKEQN 942



>NP_000680
          Length = 501

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   MRIAKE 6
           MRIAKE
Sbjct: 394 MRIAKE 399



>NP_000684
          Length = 512

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   MRIAKE 6
           MRIAKE
Sbjct: 405 MRIAKE 410



>NP_001243323
          Length = 1099

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1   MRIAKENN 8
           M IAKE N
Sbjct: 242 MMIAKEEN 249



>NP_000683
          Length = 517

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   MRIAKE 6
           MRIAKE
Sbjct: 410 MRIAKE 415



>NP_443124
          Length = 2459

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2    RIAKENN 8
            R+AKE N
Sbjct: 1621 RVAKEQN 1627



>NP_060418
          Length = 1167

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1   MRIAKENN 8
           M IAKE N
Sbjct: 310 MMIAKEEN 317



>XP_006717236
          Length = 1041

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1   MRIAKENN 8
           M IAKE N
Sbjct: 184 MMIAKEEN 191



>XP_006717235
          Length = 1041

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1   MRIAKENN 8
           M IAKE N
Sbjct: 184 MMIAKEEN 191



>XP_006717234
          Length = 1041

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1   MRIAKENN 8
           M IAKE N
Sbjct: 184 MMIAKEEN 191



>XP_006717233
          Length = 1099

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1   MRIAKENN 8
           M IAKE N
Sbjct: 242 MMIAKEEN 249



>XP_006717232
          Length = 1099

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1   MRIAKENN 8
           M IAKE N
Sbjct: 242 MMIAKEEN 249



>XP_006717231
          Length = 1129

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1   MRIAKENN 8
           M IAKE N
Sbjct: 310 MMIAKEEN 317



>XP_006717230
          Length = 1167

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1   MRIAKENN 8
           M IAKE N
Sbjct: 310 MMIAKEEN 317



>XP_006717229
          Length = 1167

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1   MRIAKENN 8
           M IAKE N
Sbjct: 310 MMIAKEEN 317



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.309    0.118    0.292 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 561,079
Number of extensions: 828
Number of successful extensions: 37
Number of sequences better than 10000.0: 37
Number of HSP's gapped: 37
Number of HSP's successfully gapped: 37
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.5 bits)
S2: 28 (15.4 bits)