Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_001243398                                                           20     390
NP_036462                                                              20     390
NP_001243397                                                           20     390
XP_005269721                                                           20     390
NP_002218                                                              17    3301
XP_005270898                                                           17    3301
XP_006710687                                                           17    3301
NP_001138537                                                           17    4312
NP_997374                                                              17    4312
NP_065853                                                              16    5631
XP_005271684                                                           16    5631
NP_000432                                                              16    7355
XP_005250483                                                           16    7355
XP_006716087                                                           16    7355
XP_005250482                                                           16    7355
NP_003611                                                              15    9605
NP_060952                                                              15    9605
NP_705900                                                              15    9605
NP_055242                                                              15    9605
NP_001073996                                                           15    9605
NP_001234925                                                           15    9605
XP_006716630                                                           15    9605
XP_006716629                                                           15    9605
XP_006716628                                                           15    9605
XP_006716627                                                           15    9605
XP_006716626                                                           15    9605
XP_005250982                                                           15    9605
XP_006712602                                                           15    9605
NP_055248                                                              15    9605
NP_665805                                                              15    9605
>NP_001243398
          Length = 1781

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    MQLAEIPKT 9
            MQLAEIP+T
Sbjct: 1487 MQLAEIPET 1495
>NP_036462
          Length = 2073

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    MQLAEIPKT 9
            MQLAEIP+T
Sbjct: 1779 MQLAEIPET 1787
>NP_001243397
          Length = 1890

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    MQLAEIPKT 9
            MQLAEIP+T
Sbjct: 1596 MQLAEIPET 1604
>XP_005269721
          Length = 2073

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    MQLAEIPKT 9
            MQLAEIP+T
Sbjct: 1779 MQLAEIPET 1787
>NP_002218
          Length = 1154

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   MQLAEIPK 8
           MQL E+PK
Sbjct: 206 MQLPELPK 213
>XP_005270898
          Length = 1154

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   MQLAEIPK 8
           MQL E+PK
Sbjct: 206 MQLPELPK 213
>XP_006710687
          Length = 1154

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   MQLAEIPK 8
           MQL E+PK
Sbjct: 206 MQLPELPK 213
>NP_001138537
          Length = 900

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   MQLAEIPKT 9
           +Q+AE+P T
Sbjct: 350 LQIAELPAT 358
>NP_997374
          Length = 677

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   MQLAEIPKT 9
           +Q+AE+P T
Sbjct: 350 LQIAELPAT 358
>NP_065853
          Length = 1117

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   AEIPKT 9
           AEIPKT
Sbjct: 621 AEIPKT 626
>XP_005271684
          Length = 1081

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   AEIPKT 9
           AEIPKT
Sbjct: 621 AEIPKT 626
>NP_000432
          Length = 780

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   MQLAEIPK 8
           MQL +IP+
Sbjct: 463 MQLCDIPR 470
>XP_005250483
          Length = 367

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1  MQLAEIPK 8
          MQL +IP+
Sbjct: 50 MQLCDIPR 57
>XP_006716087
          Length = 780

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   MQLAEIPK 8
           MQL +IP+
Sbjct: 463 MQLCDIPR 470
>XP_005250482
          Length = 780

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   MQLAEIPK 8
           MQL +IP+
Sbjct: 463 MQLCDIPR 470
>NP_003611
          Length = 605

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   QLAEIPKT 9
           +LAE PKT
Sbjct: 156 KLAEWPKT 163
>NP_060952
          Length = 1129

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   QLAEIPK 8
           QLAE+P+
Sbjct: 926 QLAELPQ 932
>NP_705900
          Length = 558

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   LAEIPK 8
           LAE+PK
Sbjct: 524 LAEVPK 529
>NP_055242
          Length = 633

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   LAEIPK 8
           LAE+PK
Sbjct: 599 LAEVPK 604
>NP_001073996
          Length = 2116

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2    QLAEIPK 8
            QLA +PK
Sbjct: 1949 QLASVPK 1955
>NP_001234925
          Length = 1122

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   QLAEIPK 8
           QLAE+P+
Sbjct: 919 QLAELPQ 925
>XP_006716630
          Length = 1073

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   QLAEIPK 8
           QLAE+P+
Sbjct: 870 QLAELPQ 876
>XP_006716629
          Length = 1075

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   QLAEIPK 8
           QLAE+P+
Sbjct: 872 QLAELPQ 878
>XP_006716628
          Length = 1076

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   QLAEIPK 8
           QLAE+P+
Sbjct: 873 QLAELPQ 879
>XP_006716627
          Length = 1125

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   QLAEIPK 8
           QLAE+P+
Sbjct: 922 QLAELPQ 928
>XP_006716626
          Length = 1132

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   QLAEIPK 8
           QLAE+P+
Sbjct: 929 QLAELPQ 935
>XP_005250982
          Length = 1132

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   QLAEIPK 8
           QLAE+P+
Sbjct: 929 QLAELPQ 935
>XP_006712602
          Length = 2142

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2    QLAEIPK 8
            QLA +PK
Sbjct: 1975 QLASVPK 1981
>NP_055248
          Length = 348

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 1   MQLAEIPK 8
           MQL ++P+
Sbjct: 133 MQLVQVPR 140
>NP_665805
          Length = 252

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 1  MQLAEIPK 8
          MQ+A++P+
Sbjct: 66 MQVADVPE 73
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.313    0.125    0.326 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 660,017
Number of extensions: 739
Number of successful extensions: 30
Number of sequences better than 10000.0: 30
Number of HSP's gapped: 30
Number of HSP's successfully gapped: 30
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.7 bits)
S2: 28 (15.4 bits)

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