Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_003310                                                              17    4312
NP_597676                                                              17    4312
NP_001243779                                                           17    4312
NP_596869                                                              17    4312
NP_060694                                                              17    4312
XP_006715815                                                           17    4312
XP_005249864                                                           17    4312
XP_006715814                                                           17    4312
XP_005249863                                                           17    4312
XP_005249861                                                           17    4312
XP_005246888                                                           17    4312
XP_006712788                                                           17    4312
XP_006712787                                                           17    4312
XP_006712786                                                           17    4312
NP_001254479                                                           17    4312
NP_597681                                                              17    4312
NP_116250                                                              16    5631
NP_060521                                                              16    5631
NP_060055                                                              16    5631
NP_859075                                                              16    5631
XP_006719432                                                           16    5631
XP_006719431                                                           16    5631
XP_005249607                                                           16    5631
XP_005249606                                                           16    5631
XP_006716104                                                           16    5631
XP_006715649                                                           16    5631
XP_006715648                                                           16    5631
NP_001258463                                                           16    7355
NP_001380                                                              16    7355
NP_055914                                                              16    7355
NP_001164639                                                           16    7355
NP_001164638                                                           16    7355
NP_055918                                                              16    7355
NP_001191675                                                           16    7355
NP_001191674                                                           16    7355
NP_001191673                                                           16    7355
NP_110429                                                              16    7355
NP_004053                                                              16    7355
XP_006724038                                                           16    7355
XP_006721545                                                           16    7355
XP_006721544                                                           16    7355
XP_006721543                                                           16    7355
XP_006721542                                                           16    7355
XP_006721541                                                           16    7355
XP_005255827                                                           16    7355
XP_005255826                                                           16    7355
XP_005255825                                                           16    7355
NP_001164637                                                           16    7355
NP_060644                                                              16    7355
NP_114414                                                              16    7355
NP_776188                                                              15    9605
XP_005265737                                                           15    9605
XP_005265736                                                           15    9605
>NP_003310
          Length = 26926

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1     MPLKATILF 9
             +PLKAT+ F
Sbjct: 20018 VPLKATMRF 20026
>NP_597676
          Length = 27051

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1     MPLKATILF 9
             +PLKAT+ F
Sbjct: 20143 VPLKATMRF 20151
>NP_001243779
          Length = 34350

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1     MPLKATILF 9
             +PLKAT+ F
Sbjct: 27442 VPLKATMRF 27450
>NP_596869
          Length = 33423

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1     MPLKATILF 9
             +PLKAT+ F
Sbjct: 26515 VPLKATMRF 26523
>NP_060694
          Length = 146

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1  MPLKATILF 9
          MPL A ILF
Sbjct: 13 MPLGARILF 21
>XP_006715815
          Length = 146

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1  MPLKATILF 9
          MPL A ILF
Sbjct: 13 MPLGARILF 21
>XP_005249864
          Length = 146

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1  MPLKATILF 9
          MPL A ILF
Sbjct: 13 MPLGARILF 21
>XP_006715814
          Length = 146

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1  MPLKATILF 9
          MPL A ILF
Sbjct: 13 MPLGARILF 21
>XP_005249863
          Length = 146

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1  MPLKATILF 9
          MPL A ILF
Sbjct: 13 MPLGARILF 21
>XP_005249861
          Length = 146

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1  MPLKATILF 9
          MPL A ILF
Sbjct: 13 MPLGARILF 21
>XP_005246888
          Length = 22663

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1     MPLKATILF 9
             +PLKAT+ F
Sbjct: 15755 VPLKATMRF 15763
>XP_006712788
          Length = 24022

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1     MPLKATILF 9
             +PLKAT+ F
Sbjct: 17114 VPLKATMRF 17122
>XP_006712787
          Length = 33978

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1     MPLKATILF 9
             +PLKAT+ F
Sbjct: 27070 VPLKATMRF 27078
>XP_006712786
          Length = 35484

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1     MPLKATILF 9
             +PLKAT+ F
Sbjct: 28576 VPLKATMRF 28584
>NP_001254479
          Length = 35991

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1     MPLKATILF 9
             +PLKAT+ F
Sbjct: 29083 VPLKATMRF 29091
>NP_597681
          Length = 27118

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1     MPLKATILF 9
             +PLKAT+ F
Sbjct: 20210 VPLKATMRF 20218
>NP_116250
          Length = 654

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   PLKATILF 9
           P+KA +LF
Sbjct: 391 PIKADVLF 398
>NP_060521
          Length = 1066

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   PLKATILF 9
           PLKA +LF
Sbjct: 706 PLKAFLLF 713
>NP_060055
          Length = 507

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   MPLKATILF 9
           +PL AT+LF
Sbjct: 397 VPLLATMLF 405
>NP_859075
          Length = 283

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1  MPLKATIL 8
          +PLKAT L
Sbjct: 28 VPLKATFL 35
>XP_006719432
          Length = 283

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1  MPLKATIL 8
          +PLKAT L
Sbjct: 28 VPLKATFL 35
>XP_006719431
          Length = 283

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1  MPLKATIL 8
          +PLKAT L
Sbjct: 28 VPLKATFL 35
>XP_005249607
          Length = 579

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   PLKATILF 9
           PLKA +LF
Sbjct: 219 PLKAFLLF 226
>XP_005249606
          Length = 878

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   PLKATILF 9
           PLKA +LF
Sbjct: 518 PLKAFLLF 525
>XP_006716104
          Length = 1069

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   PLKATILF 9
           PLKA +LF
Sbjct: 709 PLKAFLLF 716
>XP_006715649
          Length = 584

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   PLKATILF 9
           P+KA +LF
Sbjct: 321 PIKADVLF 328
>XP_006715648
          Length = 616

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   PLKATILF 9
           P+KA +LF
Sbjct: 353 PIKADVLF 360
>NP_001258463
          Length = 1994

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2   PLKATI 7
           PLKATI
Sbjct: 313 PLKATI 318
>NP_001380
          Length = 2012

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2   PLKATI 7
           PLKATI
Sbjct: 313 PLKATI 318
>NP_055914
          Length = 1202

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   PLKATILF 9
           PL A++LF
Sbjct: 605 PLSASVLF 612
>NP_001164639
          Length = 1201

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   PLKATILF 9
           PL A++LF
Sbjct: 604 PLSASVLF 611
>NP_001164638
          Length = 1241

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   PLKATILF 9
           PL A++LF
Sbjct: 628 PLSASVLF 635
>NP_055918
          Length = 1925

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   PLKATILF 9
           PLKAT +F
Sbjct: 459 PLKATPVF 466
>NP_001191675
          Length = 732

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   MPLKATI 7
           +PLKAT+
Sbjct: 726 VPLKATV 732
>NP_001191674
          Length = 790

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   MPLKATI 7
           +PLKAT+
Sbjct: 784 VPLKATV 790
>NP_001191673
          Length = 807

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   MPLKATI 7
           +PLKAT+
Sbjct: 801 VPLKATV 807
>NP_110429
          Length = 453

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2  PLKATILF 9
          PL+ATI F
Sbjct: 43 PLRATIPF 50
>NP_004053
          Length = 829

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   MPLKATI 7
           +PLKAT+
Sbjct: 823 VPLKATV 829
>XP_006724038
          Length = 1907

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2   PLKATI 7
           PLKATI
Sbjct: 208 PLKATI 213
>XP_006721545
          Length = 1015

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   PLKATILF 9
           PL A++LF
Sbjct: 418 PLSASVLF 425
>XP_006721544
          Length = 1178

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   PLKATILF 9
           PL A++LF
Sbjct: 581 PLSASVLF 588
>XP_006721543
          Length = 1195

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   PLKATILF 9
           PL A++LF
Sbjct: 605 PLSASVLF 612
>XP_006721542
          Length = 1200

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   PLKATILF 9
           PL A++LF
Sbjct: 610 PLSASVLF 617
>XP_006721541
          Length = 1207

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   PLKATILF 9
           PL A++LF
Sbjct: 610 PLSASVLF 617
>XP_005255827
          Length = 651

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   MPLKATI 7
           +PLKAT+
Sbjct: 645 VPLKATV 651
>XP_005255826
          Length = 766

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   MPLKATI 7
           +PLKAT+
Sbjct: 760 VPLKATV 766
>XP_005255825
          Length = 829

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   MPLKATI 7
           +PLKAT+
Sbjct: 823 VPLKATV 829
>NP_001164637
          Length = 1197

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   PLKATILF 9
           PL A++LF
Sbjct: 607 PLSASVLF 614
>NP_060644
          Length = 1059

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   MPLKATILF 9
           +P K T+LF
Sbjct: 377 VPAKPTVLF 385
>NP_114414
          Length = 585

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   MPLKATI 7
           MP KATI
Sbjct: 295 MPAKATI 301
>NP_776188
          Length = 333

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   PLKATIL 8
           P KAT+L
Sbjct: 167 PFKATVL 173
>XP_005265737
          Length = 320

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   PLKATIL 8
           P KAT+L
Sbjct: 167 PFKATVL 173
>XP_005265736
          Length = 334

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   PLKATIL 8
           P KAT+L
Sbjct: 167 PFKATVL 173
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.340    0.148    0.431 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 435,212
Number of extensions: 816
Number of successful extensions: 53
Number of sequences better than 10000.0: 53
Number of HSP's gapped: 53
Number of HSP's successfully gapped: 53
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.5 bits)
S2: 28 (15.4 bits)

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