Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_005742                                                              20     299
NP_671714                                                              20     299
XP_006715890                                                           20     299
XP_005250160                                                           20     299
XP_005250159                                                           20     299
NP_065813                                                              17    3301
XP_005269099                                                           17    3301
XP_005250110                                                           16    5631
XP_005250109                                                           16    5631
NP_003452                                                              16    5631
NP_001010972                                                           16    5631
NP_001177766                                                           15    9605
NP_001128512                                                           15    9605
NP_004073                                                              15    9605
NP_075408                                                              15    9605
NP_001128513                                                           15    9605
NP_001177765                                                           15    9605
NP_001316                                                              15    9605
NP_085132                                                              15    9605
NP_938204                                                              15    9605
XP_006724685                                                           15    9605
XP_006721348                                                           15    9605
XP_005256239                                                           15    9605
XP_005256238                                                           15    9605
XP_005256236                                                           15    9605
>NP_005742
          Length = 3907

 Score = 20.4 bits (41), Expect =   299
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   MNVAMNELN 9
           MNVA+NELN
Sbjct: 491 MNVAINELN 499
>NP_671714
          Length = 3899

 Score = 20.4 bits (41), Expect =   299
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   MNVAMNELN 9
           MNVA+NELN
Sbjct: 491 MNVAINELN 499
>XP_006715890
          Length = 3860

 Score = 20.4 bits (41), Expect =   299
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   MNVAMNELN 9
           MNVA+NELN
Sbjct: 491 MNVAINELN 499
>XP_005250160
          Length = 3939

 Score = 20.4 bits (41), Expect =   299
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   MNVAMNELN 9
           MNVA+NELN
Sbjct: 491 MNVAINELN 499
>XP_005250159
          Length = 3952

 Score = 20.4 bits (41), Expect =   299
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   MNVAMNELN 9
           MNVA+NELN
Sbjct: 491 MNVAINELN 499
>NP_065813
          Length = 1085

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   MNVAMNEL 8
           MN A+NEL
Sbjct: 966 MNTALNEL 973
>XP_005269099
          Length = 1062

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   MNVAMNEL 8
           MN A+NEL
Sbjct: 943 MNTALNEL 950
>XP_005250110
          Length = 631

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   NVAMNEL 8
           NVA+NEL
Sbjct: 436 NVAVNEL 442
>XP_005250109
          Length = 632

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   NVAMNEL 8
           NVA+NEL
Sbjct: 437 NVAVNEL 443
>NP_003452
          Length = 572

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   NVAMNEL 8
           NVA+NEL
Sbjct: 377 NVAVNEL 383
>NP_001010972
          Length = 572

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   NVAMNEL 8
           NVA+NEL
Sbjct: 377 NVAVNEL 383
>NP_001177766
          Length = 1271

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   AMNELN 9
           AMNE+N
Sbjct: 459 AMNEMN 464
>NP_001128512
          Length = 1253

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   AMNELN 9
           AMNE+N
Sbjct: 446 AMNEMN 451
>NP_004073
          Length = 1278

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   AMNELN 9
           AMNE+N
Sbjct: 466 AMNEMN 471
>NP_075408
          Length = 1144

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   AMNELN 9
           AMNE+N
Sbjct: 332 AMNEMN 337
>NP_001128513
          Length = 1139

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   AMNELN 9
           AMNE+N
Sbjct: 332 AMNEMN 337
>NP_001177765
          Length = 1236

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   AMNELN 9
           AMNE+N
Sbjct: 429 AMNEMN 434
>NP_001316
          Length = 577

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   NVAMNELN 9
           NV+MN+ N
Sbjct: 218 NVSMNQQN 225
>NP_085132
          Length = 679

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   VAMNELN 9
           +A+NELN
Sbjct: 198 LALNELN 204
>NP_938204
          Length = 773

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   VAMNELN 9
           +A+NELN
Sbjct: 292 LALNELN 298
>XP_006724685
          Length = 417

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2  NVAMNELN 9
          NV+MN+ N
Sbjct: 58 NVSMNQQN 65
>XP_006721348
          Length = 586

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   VAMNELN 9
           +A+NELN
Sbjct: 105 LALNELN 111
>XP_005256239
          Length = 586

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   VAMNELN 9
           +A+NELN
Sbjct: 105 LALNELN 111
>XP_005256238
          Length = 586

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   VAMNELN 9
           +A+NELN
Sbjct: 105 LALNELN 111
>XP_005256236
          Length = 737

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   VAMNELN 9
           +A+NELN
Sbjct: 256 LALNELN 262
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.311    0.118    0.298 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 281,734
Number of extensions: 451
Number of successful extensions: 25
Number of sequences better than 10000.0: 25
Number of HSP's gapped: 25
Number of HSP's successfully gapped: 25
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.6 bits)
S2: 28 (15.4 bits)

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