Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_064450                                                              22     134
XP_005273257                                                           22     134
NP_064630                                                              18    1935
NP_071938                                                              18    1935
XP_005276653                                                           18    1935
XP_005256952                                                           18    1935
XP_005267114                                                           18    1935
XP_005275865                                                           18    1935
NP_001757                                                              17    2528
NP_640339                                                              17    2528
NP_742066                                                              17    2528
XP_006717042                                                           17    2528
XP_005251797                                                           17    2528
XP_006712004                                                           17    2528
XP_006711684                                                           17    2528
XP_005245640                                                           17    2528
XP_006711683                                                           17    2528
NP_001078956                                                           17    3301
XP_006710377                                                           17    3301
NP_001135885                                                           17    4312
NP_005131                                                              17    4312
XP_006719940                                                           17    4312
NP_113619                                                              17    4312
NP_001001938                                                           17    4312
NP_001127807                                                           16    5631
NP_055228                                                              16    5631
NP_060897                                                              16    5631
NP_001076                                                              16    5631
XP_006726677                                                           16    5631
XP_005276561                                                           16    5631
XP_005276560                                                           16    5631
XP_003960700                                                           16    5631
XP_006725262                                                           16    5631
XP_006725261                                                           16    5631
XP_006725260                                                           16    5631
XP_005255434                                                           16    5631
XP_005255433                                                           16    5631
XP_005255432                                                           16    5631
NP_057511                                                              16    5631
NP_085128                                                              16    7355
NP_001139627                                                           16    7355
NP_001139626                                                           16    7355
NP_689657                                                              16    7355
NP_653267                                                              16    7355
NP_777597                                                              16    7355
NP_079105                                                              16    7355
NP_001157569                                                           15    9605
NP_004376                                                              15    9605
NP_001106962                                                           15    9605
NP_001123882                                                           15    9605
NP_001598                                                              15    9605
NP_653205                                                              15    9605
NP_055073                                                              15    9605
NP_077015                                                              15    9605
XP_005262146                                                           15    9605
XP_005262145                                                           15    9605
XP_005262144                                                           15    9605
XP_006723744                                                           15    9605
XP_005260304                                                           15    9605
XP_005256339                                                           15    9605
XP_005251349                                                           15    9605
XP_005251348                                                           15    9605
XP_006715718                                                           15    9605
XP_006713186                                                           15    9605
XP_006713185                                                           15    9605
NP_001244899                                                           15    9605
>NP_064450
          Length = 1722

 Score = 21.6 bits (44), Expect =   134
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    MNQDKGTRK 9
            MNQDKG+RK
Sbjct: 1276 MNQDKGSRK 1284
>XP_005273257
          Length = 1726

 Score = 21.6 bits (44), Expect =   134
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    MNQDKGTRK 9
            MNQDKG+RK
Sbjct: 1280 MNQDKGSRK 1288
>NP_064630
          Length = 1543

 Score = 17.7 bits (34), Expect =  1935
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1    MNQDKGTRK 9
            MNQ +G+RK
Sbjct: 1420 MNQSQGSRK 1428
>NP_071938
          Length = 476

 Score = 17.7 bits (34), Expect =  1935
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1  MNQDKGTRK 9
          +NQD GTR+
Sbjct: 52 INQDGGTRR 60
>XP_005276653
          Length = 195

 Score = 17.7 bits (34), Expect =  1935
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2  NQDKGTRK 9
          NQD GTR+
Sbjct: 73 NQDMGTRR 80
>XP_005256952
          Length = 195

 Score = 17.7 bits (34), Expect =  1935
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2  NQDKGTRK 9
          NQD GTR+
Sbjct: 73 NQDMGTRR 80
>XP_005267114
          Length = 1543

 Score = 17.7 bits (34), Expect =  1935
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1    MNQDKGTRK 9
            MNQ +G+RK
Sbjct: 1420 MNQSQGSRK 1428
>XP_005275865
          Length = 195

 Score = 17.7 bits (34), Expect =  1935
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2  NQDKGTRK 9
          NQD GTR+
Sbjct: 73 NQDMGTRR 80
>NP_001757
          Length = 335

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   MNQDKGTRK 9
           +NQDK TR+
Sbjct: 166 LNQDKWTRE 174
>NP_640339
          Length = 516

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   QDKGTRK 9
           QDKG+RK
Sbjct: 460 QDKGSRK 466
>NP_742066
          Length = 1493

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1    MNQDKGTRK 9
            +NQD G RK
Sbjct: 1150 LNQDTGMRK 1158
>XP_006717042
          Length = 290

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   QDKGTRK 9
           QDKG+RK
Sbjct: 234 QDKGSRK 240
>XP_005251797
          Length = 475

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   QDKGTRK 9
           QDKG+RK
Sbjct: 419 QDKGSRK 425
>XP_006712004
          Length = 1488

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1    MNQDKGTRK 9
            +NQD G RK
Sbjct: 1145 LNQDTGMRK 1153
>XP_006711684
          Length = 231

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1  MNQDKGTRK 9
          +NQDK TR+
Sbjct: 62 LNQDKWTRE 70
>XP_005245640
          Length = 231

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1  MNQDKGTRK 9
          +NQDK TR+
Sbjct: 62 LNQDKWTRE 70
>XP_006711683
          Length = 242

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   MNQDKGTRK 9
           +NQDK TR+
Sbjct: 166 LNQDKWTRE 174
>NP_001078956
          Length = 828

 Score = 16.9 bits (32), Expect =  3301
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   NQDKGTR 8
           N+DKGT+
Sbjct: 188 NEDKGTK 194
>XP_006710377
          Length = 827

 Score = 16.9 bits (32), Expect =  3301
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   NQDKGTR 8
           N+DKGT+
Sbjct: 188 NEDKGTK 194
>NP_001135885
          Length = 183

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2  NQDKGTRK 9
          N+D GTR+
Sbjct: 74 NRDSGTRR 81
>NP_005131
          Length = 664

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   QDKGTRK 9
           +DKGT+K
Sbjct: 120 EDKGTKK 126
>XP_006719940
          Length = 820

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   MNQDKGTR 8
           M+QD+GT+
Sbjct: 119 MDQDEGTK 126
>NP_113619
          Length = 828

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   MNQDKGTR 8
           M+QD+GT+
Sbjct: 119 MDQDEGTK 126
>NP_001001938
          Length = 202

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   NQDKGTRK 9
           N+D GTR+
Sbjct: 93  NRDSGTRR 100
>NP_001127807
          Length = 629

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   MNQDKGTR 8
           ++QDKG R
Sbjct: 613 LSQDKGVR 620
>NP_055228
          Length = 630

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   MNQDKGTR 8
           ++QDKG R
Sbjct: 614 LSQDKGVR 621
>NP_060897
          Length = 651

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   NQDKGTR 8
           NQD GT+
Sbjct: 439 NQDSGTK 445
>NP_001076
          Length = 423

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2  NQDKGT 7
          NQD+GT
Sbjct: 38 NQDRGT 43
>XP_006726677
          Length = 213

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2  NQDKGTR 8
          NQD GT+
Sbjct: 83 NQDSGTK 89
>XP_005276561
          Length = 220

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2  NQDKGTR 8
          NQD GT+
Sbjct: 83 NQDSGTK 89
>XP_005276560
          Length = 224

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2  NQDKGTR 8
          NQD GT+
Sbjct: 83 NQDSGTK 89
>XP_003960700
          Length = 247

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2  NQDKGTR 8
          NQD GT+
Sbjct: 83 NQDSGTK 89
>XP_006725262
          Length = 439

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   NQDKGTR 8
           NQD GT+
Sbjct: 227 NQDSGTK 233
>XP_006725261
          Length = 439

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   NQDKGTR 8
           NQD GT+
Sbjct: 227 NQDSGTK 233
>XP_006725260
          Length = 621

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   NQDKGTR 8
           NQD GT+
Sbjct: 409 NQDSGTK 415
>XP_005255434
          Length = 439

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   NQDKGTR 8
           NQD GT+
Sbjct: 227 NQDSGTK 233
>XP_005255433
          Length = 439

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   NQDKGTR 8
           NQD GT+
Sbjct: 227 NQDSGTK 233
>XP_005255432
          Length = 621

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   NQDKGTR 8
           NQD GT+
Sbjct: 409 NQDSGTK 415
>NP_057511
          Length = 753

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   NQDKGTRK 9
           N+ KGTR+
Sbjct: 415 NESKGTRE 422
>NP_085128
          Length = 2136

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2    NQDKGT 7
            NQ+KGT
Sbjct: 1117 NQEKGT 1122
>NP_001139627
          Length = 351

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1  MNQDKGT 7
          MN+D GT
Sbjct: 56 MNEDTGT 62
>NP_001139626
          Length = 351

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1  MNQDKGT 7
          MN+D GT
Sbjct: 56 MNEDTGT 62
>NP_689657
          Length = 351

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1  MNQDKGT 7
          MN+D GT
Sbjct: 56 MNEDTGT 62
>NP_653267
          Length = 4753

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1    MNQDKGTR 8
            + Q+KGTR
Sbjct: 2768 IRQEKGTR 2775
>NP_777597
          Length = 586

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   QDKGTR 8
           +DKGTR
Sbjct: 435 EDKGTR 440
>NP_079105
          Length = 553

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   QDKGTRK 9
           QDK TRK
Sbjct: 142 QDKWTRK 148
>NP_001157569
          Length = 2409

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2   NQDKGTRK 9
           + D GTRK
Sbjct: 777 SSDSGTRK 784
>NP_004376
          Length = 3396

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2    NQDKGTRK 9
            + D GTRK
Sbjct: 1764 SSDSGTRK 1771
>NP_001106962
          Length = 1084

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   MNQDKGTR 8
           M+QD+G R
Sbjct: 157 MHQDEGLR 164
>NP_001123882
          Length = 331

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1   MNQDKGTRK 9
           ++ DKGT++
Sbjct: 197 VHDDKGTKR 205
>NP_001598
          Length = 424

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1   MNQDKGTRK 9
           ++ DKGT++
Sbjct: 230 VHDDKGTKR 238
>NP_653205
          Length = 953

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   DKGTRK 9
           DKG+RK
Sbjct: 826 DKGSRK 831
>NP_055073
          Length = 1530

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 5/7 (71%)

Query: 2   NQDKGTR 8
           N DKG R
Sbjct: 456 NSDKGNR 462
>NP_077015
          Length = 1004

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1   MNQDKGTRK 9
           +NQ+KG ++
Sbjct: 139 LNQEKGQKE 147
>XP_005262146
          Length = 1084

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   MNQDKGTR 8
           M+QD+G R
Sbjct: 157 MHQDEGLR 164
>XP_005262145
          Length = 1084

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   MNQDKGTR 8
           M+QD+G R
Sbjct: 157 MHQDEGLR 164
>XP_005262144
          Length = 1084

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   MNQDKGTR 8
           M+QD+G R
Sbjct: 157 MHQDEGLR 164
>XP_006723744
          Length = 847

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 5/7 (71%)

Query: 2   NQDKGTR 8
           N DKG R
Sbjct: 456 NSDKGNR 462
>XP_005260304
          Length = 1530

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 5/7 (71%)

Query: 2   NQDKGTR 8
           N DKG R
Sbjct: 456 NSDKGNR 462
>XP_005256339
          Length = 977

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   DKGTRK 9
           DKG+RK
Sbjct: 850 DKGSRK 855
>XP_005251349
          Length = 449

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   MNQDKGTRK 9
           MN+ +G RK
Sbjct: 337 MNKKEGVRK 345
>XP_005251348
          Length = 469

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   MNQDKGTRK 9
           MN+ +G RK
Sbjct: 357 MNKKEGVRK 365
>XP_006715718
          Length = 392

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   QDKGTRK 9
           QD GTR+
Sbjct: 105 QDPGTRR 111
>XP_006713186
          Length = 272

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1  MNQDKGTRK 9
          ++ DKGT++
Sbjct: 78 VHDDKGTKR 86
>XP_006713185
          Length = 364

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1   MNQDKGTRK 9
           ++ DKGT++
Sbjct: 230 VHDDKGTKR 238
>NP_001244899
          Length = 740

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1   MNQDKGTRK 9
           +NQ+KG ++
Sbjct: 139 LNQEKGQKE 147
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.305    0.121    0.329 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 653,267
Number of extensions: 1089
Number of successful extensions: 66
Number of sequences better than 10000.0: 66
Number of HSP's gapped: 66
Number of HSP's successfully gapped: 66
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.4 bits)
S2: 28 (15.4 bits)

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