Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_006209                                                              21     229
XP_006713721                                                           21     229
NP_076425                                                              17    2528
XP_005255569                                                           17    2528
XP_005255568                                                           17    2528
NP_001135768                                                           17    4312
NP_001135766                                                           17    4312
NP_001135767                                                           17    4312
NP_055902                                                              17    4312
XP_005266373                                                           17    4312
XP_005266372                                                           17    4312
XP_005266374                                                           17    4312
XP_005266370                                                           17    4312
XP_005266371                                                           17    4312
NP_078966                                                              16    5631
NP_690621                                                              16    5631
NP_690620                                                              16    5631
NP_690619                                                              16    5631
NP_061956                                                              16    5631
NP_002812                                                              16    5631
NP_001257327                                                           16    5631
XP_005263127                                                           16    5631
XP_005265836                                                           16    5631
XP_005265835                                                           16    5631
XP_005271003                                                           16    5631
XP_005271002                                                           16    5631
NP_061908                                                              16    5631
NP_078858                                                              16    7355
NP_001278232                                                           16    7355
NP_001278214                                                           16    7355
NP_115661                                                              16    7355
NP_001026897                                                           16    7355
NP_057382                                                              16    7355
NP_076411                                                              16    7355
NP_001092010                                                           15    9605
NP_001159970                                                           15    9605
NP_001186759                                                           15    9605
NP_006656                                                              15    9605
NP_710159                                                              15    9605
NP_060590                                                              15    9605
NP_277045                                                              15    9605
NP_006157                                                              15    9605
NP_001615                                                              15    9605
NP_003128                                                              15    9605
NP_489479                                                              15    9605
NP_891977                                                              15    9605
NP_150287                                                              15    9605
NP_892114                                                              15    9605
XP_006720302                                                           15    9605
XP_005268074                                                           15    9605
XP_005268966                                                           15    9605
XP_005268967                                                           15    9605
XP_005268965                                                           15    9605
XP_005251321                                                           15    9605
XP_006716521                                                           15    9605
XP_005266896                                                           15    9605
>NP_006209
          Length = 1068

 Score = 20.8 bits (42), Expect =   229
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   MNGDTSTKS 9
           MNG+TSTKS
Sbjct: 318 MNGETSTKS 326
>XP_006713721
          Length = 1068

 Score = 20.8 bits (42), Expect =   229
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   MNGDTSTKS 9
           MNG+TSTKS
Sbjct: 318 MNGETSTKS 326
>NP_076425
          Length = 218

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   NGDTSTK 8
           NGDTST+
Sbjct: 181 NGDTSTE 187
>XP_005255569
          Length = 225

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   NGDTSTK 8
           NGDTST+
Sbjct: 188 NGDTSTE 194
>XP_005255568
          Length = 248

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   NGDTSTK 8
           NGDTST+
Sbjct: 211 NGDTSTE 217
>NP_001135768
          Length = 666

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   MNGDTSTKS 9
           +NG+TST S
Sbjct: 141 VNGNTSTPS 149
>NP_001135766
          Length = 666

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   MNGDTSTKS 9
           +NG+TST S
Sbjct: 141 VNGNTSTPS 149
>NP_001135767
          Length = 666

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   MNGDTSTKS 9
           +NG+TST S
Sbjct: 141 VNGNTSTPS 149
>NP_055902
          Length = 666

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   MNGDTSTKS 9
           +NG+TST S
Sbjct: 141 VNGNTSTPS 149
>XP_005266373
          Length = 666

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   MNGDTSTKS 9
           +NG+TST S
Sbjct: 141 VNGNTSTPS 149
>XP_005266372
          Length = 666

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   MNGDTSTKS 9
           +NG+TST S
Sbjct: 141 VNGNTSTPS 149
>XP_005266374
          Length = 666

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   MNGDTSTKS 9
           +NG+TST S
Sbjct: 141 VNGNTSTPS 149
>XP_005266370
          Length = 666

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   MNGDTSTKS 9
           +NG+TST S
Sbjct: 141 VNGNTSTPS 149
>XP_005266371
          Length = 666

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   MNGDTSTKS 9
           +NG+TST S
Sbjct: 141 VNGNTSTPS 149
>NP_078966
          Length = 14507

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1    MNGDTSTKS 9
            M+ DTST+S
Sbjct: 6419 MSPDTSTRS 6427
>NP_690621
          Length = 1014

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   MNGDTSTKS 9
           + GD STKS
Sbjct: 923 LEGDFSTKS 931
>NP_690620
          Length = 940

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   MNGDTSTKS 9
           + GD STKS
Sbjct: 849 LEGDFSTKS 857
>NP_690619
          Length = 1030

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   MNGDTSTKS 9
           + GD STKS
Sbjct: 939 LEGDFSTKS 947
>NP_061956
          Length = 481

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   NGDTSTK 8
           NGD++TK
Sbjct: 156 NGDSATK 162
>NP_002812
          Length = 1070

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   MNGDTSTKS 9
           + GD STKS
Sbjct: 979 LEGDFSTKS 987
>NP_001257327
          Length = 1078

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   MNGDTSTKS 9
           + GD STKS
Sbjct: 987 LEGDFSTKS 995
>XP_005263127
          Length = 1134

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3    GDTSTKS 9
            GDTST S
Sbjct: 996  GDTSTSS 1002
>XP_005265836
          Length = 549

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   MNGDTSTKS 9
           + GDT+TK+
Sbjct: 488 VTGDTNTKN 496
>XP_005265835
          Length = 558

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   MNGDTSTKS 9
           + GDT+TK+
Sbjct: 497 VTGDTNTKN 505
>XP_005271003
          Length = 340

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2  NGDTSTK 8
          NGD++TK
Sbjct: 15 NGDSATK 21
>XP_005271002
          Length = 467

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   NGDTSTK 8
           NGD++TK
Sbjct: 142 NGDSATK 148
>NP_061908
          Length = 1135

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3    GDTSTKS 9
            GDTST S
Sbjct: 997  GDTSTSS 1003
>NP_078858
          Length = 4981

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1    MNGDTST 7
            +NGDT T
Sbjct: 1568 INGDTDT 1574
>NP_001278232
          Length = 4983

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1    MNGDTST 7
            +NGDT T
Sbjct: 1568 INGDTDT 1574
>NP_001278214
          Length = 4982

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1    MNGDTST 7
            +NGDT T
Sbjct: 1568 INGDTDT 1574
>NP_115661
          Length = 322

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   NGDTSTK 8
           NGDT+ K
Sbjct: 206 NGDTANK 212
>NP_001026897
          Length = 369

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   NGDTSTK 8
           NGDT+ K
Sbjct: 253 NGDTANK 259
>NP_057382
          Length = 709

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   MNGDTSTK 8
           MNG+TS +
Sbjct: 160 MNGETSVE 167
>NP_076411
          Length = 317

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   MNGDTSTKS 9
           ++GD STK+
Sbjct: 218 ISGDASTKA 226
>NP_001092010
          Length = 543

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   MNGDTSTK 8
           +NG T+TK
Sbjct: 300 LNGRTATK 307
>NP_001159970
          Length = 469

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   MNGDTSTK 8
           +NG T+TK
Sbjct: 300 LNGRTATK 307
>NP_001186759
          Length = 485

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   MNGDTSTK 8
           +NG T+TK
Sbjct: 242 LNGRTATK 249
>NP_006656
          Length = 543

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   MNGDTSTK 8
           +NG T+TK
Sbjct: 300 LNGRTATK 307
>NP_710159
          Length = 476

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   GDTSTKS 9
           GDT T+S
Sbjct: 153 GDTGTRS 159
>NP_060590
          Length = 282

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1 MNGDTSTKS 9
         MN  TST S
Sbjct: 1 MNSSTSTMS 9
>NP_277045
          Length = 1401

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1 MNGDTSTKS 9
         M+GD+S+ S
Sbjct: 1 MDGDSSSSS 9
>NP_006157
          Length = 207

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1 MNGDTST 7
         M+GD+ST
Sbjct: 3 MDGDSST 9
>NP_001615
          Length = 1723

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   MNGDTS 6
           MNGD+S
Sbjct: 359 MNGDSS 364
>NP_003128
          Length = 655

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   NGDTST 7
           NGD+ST
Sbjct: 402 NGDSST 407
>NP_489479
          Length = 924

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   NGDTST 7
           NG+TST
Sbjct: 118 NGETST 123
>NP_891977
          Length = 921

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   NGDTST 7
           NG+TST
Sbjct: 118 NGETST 123
>NP_150287
          Length = 925

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   NGDTST 7
           NG+TST
Sbjct: 118 NGETST 123
>NP_892114
          Length = 927

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   NGDTST 7
           NG+TST
Sbjct: 118 NGETST 123
>XP_006720302
          Length = 927

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   NGDTST 7
           NG+TST
Sbjct: 118 NGETST 123
>XP_005268074
          Length = 928

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   NGDTST 7
           NG+TST
Sbjct: 118 NGETST 123
>XP_005268966
          Length = 208

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1  MNGDTST 7
          M+GD+ST
Sbjct: 4  MDGDSST 10
>XP_005268967
          Length = 208

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1  MNGDTST 7
          M+GD+ST
Sbjct: 4  MDGDSST 10
>XP_005268965
          Length = 208

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1  MNGDTST 7
          M+GD+ST
Sbjct: 4  MDGDSST 10
>XP_005251321
          Length = 243

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1 MNGDTSTKS 9
         MN  TST S
Sbjct: 1 MNSSTSTMS 9
>XP_006716521
          Length = 282

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1 MNGDTSTKS 9
         MN  TST S
Sbjct: 1 MNSSTSTMS 9
>XP_005266896
          Length = 2131

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   MNGDTS 6
           MNGD+S
Sbjct: 767 MNGDSS 772
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.287    0.103    0.257 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 658,271
Number of extensions: 1267
Number of successful extensions: 56
Number of sequences better than 10000.0: 56
Number of HSP's gapped: 56
Number of HSP's successfully gapped: 56
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 17 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (14.9 bits)
S2: 28 (15.4 bits)

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