Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_004963                                                              20     390
XP_005251512                                                           20     390
NP_000124                                                              17    3301
XP_005262454                                                           17    3301
NP_068770                                                              17    4312
XP_006716795                                                           17    4312
NP_065871                                                              16    5631
NP_072174                                                              16    5631
XP_006713136                                                           16    5631
XP_006712785                                                           16    5631
XP_005246883                                                           16    5631
XP_005246882                                                           16    5631
XP_006712784                                                           16    5631
NP_001419                                                              16    7355
NP_009057                                                              16    7355
NP_699177                                                              16    7355
XP_006710496                                                           16    7355
NP_002687                                                              16    7355
NP_115532                                                              15    9605
NP_076414                                                              15    9605
NP_001002259                                                           15    9605
NP_001177779                                                           15    9605
NP_001177776                                                           15    9605
NP_037448                                                              15    9605
NP_001177778                                                           15    9605
NP_001193785                                                           15    9605
NP_001245245                                                           15    9605
NP_060568                                                              15    9605
NP_001245244                                                           15    9605
XP_006719211                                                           15    9605
XP_006719210                                                           15    9605
XP_006719209                                                           15    9605
XP_006719208                                                           15    9605
XP_006719207                                                           15    9605
XP_006719206                                                           15    9605
XP_006719205                                                           15    9605
XP_006719204                                                           15    9605
XP_006719203                                                           15    9605
XP_006719202                                                           15    9605
XP_006719201                                                           15    9605
XP_006719200                                                           15    9605
XP_005247237                                                           15    9605
XP_006713587                                                           15    9605
XP_005247233                                                           15    9605
XP_006713586                                                           15    9605
>NP_004963
          Length = 1132

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 2  MTEMEGTS 9
          MTEMEGTS
Sbjct: 8  MTEMEGTS 15
>XP_005251512
          Length = 1132

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 2  MTEMEGTS 9
          MTEMEGTS
Sbjct: 8  MTEMEGTS 15
>NP_000124
          Length = 461

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2   MTEMEGTS 9
           +TE+EGTS
Sbjct: 416 VTEVEGTS 423
>XP_005262454
          Length = 418

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2   MTEMEGTS 9
           +TE+EGTS
Sbjct: 373 VTEVEGTS 380
>NP_068770
          Length = 373

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   MMTEMEGTS 9
           +MTE  GTS
Sbjct: 158 LMTECSGTS 166
>XP_006716795
          Length = 374

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   MMTEMEGTS 9
           +MTE  GTS
Sbjct: 158 LMTECSGTS 166
>NP_065871
          Length = 1659

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   EMEGTS 9
           EMEGTS
Sbjct: 600 EMEGTS 605
>NP_072174
          Length = 1735

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   EMEGTS 9
           EMEGTS
Sbjct: 955 EMEGTS 960
>XP_006713136
          Length = 1022

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2 MTEMEGT 8
         MT+M+GT
Sbjct: 1 MTDMDGT 7
>XP_006712785
          Length = 1352

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   EMEGTS 9
           EMEGTS
Sbjct: 528 EMEGTS 533
>XP_005246883
          Length = 1352

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   EMEGTS 9
           EMEGTS
Sbjct: 528 EMEGTS 533
>XP_005246882
          Length = 1518

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   EMEGTS 9
           EMEGTS
Sbjct: 694 EMEGTS 699
>XP_006712784
          Length = 1784

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   EMEGTS 9
           EMEGTS
Sbjct: 960 EMEGTS 965
>NP_001419
          Length = 434

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   MMTEMEGT 8
           +M EM+GT
Sbjct: 93  LMIEMDGT 100
>NP_009057
          Length = 806

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   MMTEMEGTS 9
           ++TEM+G S
Sbjct: 604 ILTEMDGMS 612
>NP_699177
          Length = 799

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   MMTEMEGTS 9
           ++TEM GT+
Sbjct: 349 ILTEMPGTT 357
>XP_006710496
          Length = 434

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   MMTEMEGT 8
           +M EM+GT
Sbjct: 93  LMIEMDGT 100
>NP_002687
          Length = 172

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1  MMTEMEGT 8
          + TE+EGT
Sbjct: 30 LFTEVEGT 37
>NP_115532
          Length = 960

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 1   MMTEMEGT 8
           +MT+++GT
Sbjct: 623 LMTQIQGT 630
>NP_076414
          Length = 1077

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 1   MMTEMEGT 8
           +MT+++GT
Sbjct: 623 LMTQIQGT 630
>NP_001002259
          Length = 1127

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 1   MMTEMEGT 8
           +MT+++GT
Sbjct: 623 LMTQIQGT 630
>NP_001177779
          Length = 713

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   MTEMEGTS 9
           +TEM+G S
Sbjct: 641 LTEMDGPS 648
>NP_001177776
          Length = 642

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   MTEMEGTS 9
           +TEM+G S
Sbjct: 570 LTEMDGPS 577
>NP_037448
          Length = 639

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   MTEMEGTS 9
           +TEM+G S
Sbjct: 567 LTEMDGPS 574
>NP_001177778
          Length = 710

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   MTEMEGTS 9
           +TEM+G S
Sbjct: 638 LTEMDGPS 645
>NP_001193785
          Length = 905

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 1   MMTEMEGT 8
           +MT+++GT
Sbjct: 623 LMTQIQGT 630
>NP_001245245
          Length = 883

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   MTEMEG 7
           MTEM+G
Sbjct: 261 MTEMQG 266
>NP_060568
          Length = 883

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   MTEMEG 7
           MTEM+G
Sbjct: 261 MTEMQG 266
>NP_001245244
          Length = 914

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   MTEMEG 7
           MTEM+G
Sbjct: 292 MTEMQG 297
>XP_006719211
          Length = 794

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 1   MMTEMEGT 8
           +MT+++GT
Sbjct: 290 LMTQIQGT 297
>XP_006719210
          Length = 940

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 1   MMTEMEGT 8
           +MT+++GT
Sbjct: 623 LMTQIQGT 630
>XP_006719209
          Length = 941

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 1   MMTEMEGT 8
           +MT+++GT
Sbjct: 623 LMTQIQGT 630
>XP_006719208
          Length = 961

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 1   MMTEMEGT 8
           +MT+++GT
Sbjct: 623 LMTQIQGT 630
>XP_006719207
          Length = 1043

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 1   MMTEMEGT 8
           +MT+++GT
Sbjct: 623 LMTQIQGT 630
>XP_006719206
          Length = 1046

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 1   MMTEMEGT 8
           +MT+++GT
Sbjct: 542 LMTQIQGT 549
>XP_006719205
          Length = 1057

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 1   MMTEMEGT 8
           +MT+++GT
Sbjct: 623 LMTQIQGT 630
>XP_006719204
          Length = 1078

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 1   MMTEMEGT 8
           +MT+++GT
Sbjct: 623 LMTQIQGT 630
>XP_006719203
          Length = 1091

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 1   MMTEMEGT 8
           +MT+++GT
Sbjct: 623 LMTQIQGT 630
>XP_006719202
          Length = 1092

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 1   MMTEMEGT 8
           +MT+++GT
Sbjct: 623 LMTQIQGT 630
>XP_006719201
          Length = 1106

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 1   MMTEMEGT 8
           +MT+++GT
Sbjct: 623 LMTQIQGT 630
>XP_006719200
          Length = 1126

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 1   MMTEMEGT 8
           +MT+++GT
Sbjct: 623 LMTQIQGT 630
>XP_005247237
          Length = 757

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2  MTEMEG 7
          MTEM+G
Sbjct: 91 MTEMQG 96
>XP_006713587
          Length = 889

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   MTEMEG 7
           MTEM+G
Sbjct: 292 MTEMQG 297
>XP_005247233
          Length = 927

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   MTEMEG 7
           MTEM+G
Sbjct: 261 MTEMQG 266
>XP_006713586
          Length = 958

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   MTEMEG 7
           MTEM+G
Sbjct: 292 MTEMQG 297
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.301    0.107    0.261 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 531,680
Number of extensions: 660
Number of successful extensions: 45
Number of sequences better than 10000.0: 45
Number of HSP's gapped: 45
Number of HSP's successfully gapped: 45
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 17 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.4 bits)
S2: 28 (15.4 bits)

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