Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_000305                                                              19     665
XP_006717989                                                           19     665
NP_116231                                                              17    2528
XP_005250714                                                           17    2528
XP_005250712                                                           17    2528
NP_005325                                                              15    9605
NP_940905                                                              15    9605
NP_001274748                                                           15    9605
NP_001274749                                                           15    9605
NP_001121367                                                           15    9605
NP_001121368                                                           15    9605
NP_071912                                                              15    9605
NP_066918                                                              15    9605
XP_005274721                                                           15    9605
XP_006724879                                                           15    9605
XP_006724878                                                           15    9605
XP_006712194                                                           15    9605
XP_006712193                                                           15    9605
XP_006712192                                                           15    9605
XP_006712191                                                           15    9605
XP_006711358                                                           15    9605
XP_006711357                                                           15    9605
>NP_000305
          Length = 403

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   MMFETILMF 9
           MMFETI MF
Sbjct: 198 MMFETIPMF 206
>XP_006717989
          Length = 388

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   MMFETILMF 9
           MMFETI MF
Sbjct: 183 MMFETIPMF 191
>NP_116231
          Length = 561

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   MFETILMF 9
           MF T+LMF
Sbjct: 524 MFHTLLMF 531
>XP_005250714
          Length = 519

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   MFETILMF 9
           MF T+LMF
Sbjct: 482 MFHTLLMF 489
>XP_005250712
          Length = 560

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   MFETILMF 9
           MF T+LMF
Sbjct: 523 MFHTLLMF 530
>NP_005325
          Length = 2035

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   MMFETILM 8
           M +ETILM
Sbjct: 304 MAWETILM 311
>NP_940905
          Length = 1135

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   MFETILM 8
           +FETIL+
Sbjct: 116 LFETILL 122
>NP_001274748
          Length = 1111

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   MFETILM 8
           +FETIL+
Sbjct: 116 LFETILL 122
>NP_001274749
          Length = 1068

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   MFETILM 8
           +FETIL+
Sbjct: 116 LFETILL 122
>NP_001121367
          Length = 424

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2  MFETILMF 9
          MF +IL+F
Sbjct: 13 MFSSILLF 20
>NP_001121368
          Length = 424

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2  MFETILMF 9
          MF +IL+F
Sbjct: 13 MFSSILLF 20
>NP_071912
          Length = 424

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2  MFETILMF 9
          MF +IL+F
Sbjct: 13 MFSSILLF 20
>NP_066918
          Length = 635

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 2   MFETILMF 9
           +F+T++MF
Sbjct: 204 VFQTLVMF 211
>XP_005274721
          Length = 2036

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   MMFETILM 8
           M +ETILM
Sbjct: 304 MAWETILM 311
>XP_006724879
          Length = 2079

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   MMFETILM 8
           M +ETILM
Sbjct: 304 MAWETILM 311
>XP_006724878
          Length = 2080

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   MMFETILM 8
           M +ETILM
Sbjct: 304 MAWETILM 311
>XP_006712194
          Length = 362

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 2   MFETILMF 9
           +F+T++MF
Sbjct: 204 VFQTLVMF 211
>XP_006712193
          Length = 635

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 2   MFETILMF 9
           +F+T++MF
Sbjct: 204 VFQTLVMF 211
>XP_006712192
          Length = 635

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 2   MFETILMF 9
           +F+T++MF
Sbjct: 204 VFQTLVMF 211
>XP_006712191
          Length = 635

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 2   MFETILMF 9
           +F+T++MF
Sbjct: 204 VFQTLVMF 211
>XP_006711358
          Length = 1118

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   MFETILM 8
           +FETIL+
Sbjct: 116 LFETILL 122
>XP_006711357
          Length = 1142

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   MFETILM 8
           +FETIL+
Sbjct: 116 LFETILL 122
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.364    0.157    0.445 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 284,941
Number of extensions: 334
Number of successful extensions: 22
Number of sequences better than 10000.0: 22
Number of HSP's gapped: 22
Number of HSP's successfully gapped: 22
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 14 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (17.4 bits)
S2: 28 (15.4 bits)

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