Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_733751                                                              18    1482
XP_006716142                                                           18    1482
XP_005250088                                                           18    1482
XP_006716141                                                           18    1482
XP_005250085                                                           18    1482
XP_006716140                                                           18    1482
XP_005250084                                                           18    1482
XP_005250083                                                           18    1482
XP_005250082                                                           18    1482
NP_001035521                                                           17    4312
NP_004930                                                              16    5631
NP_001129628                                                           16    5631
NP_001254514                                                           16    5631
NP_001254515                                                           16    5631
NP_001269469                                                           16    5631
NP_060700                                                              16    5631
NP_055725                                                              16    5631
XP_006721485                                                           16    5631
XP_006721484                                                           16    5631
XP_006721483                                                           16    5631
NP_006373                                                              16    5631
NP_001141                                                              16    7355
NP_065073                                                              16    7355
XP_005254949                                                           16    7355
XP_006715582                                                           16    7355
NP_001123517                                                           15    9605
NP_001139627                                                           15    9605
NP_001139626                                                           15    9605
NP_689657                                                              15    9605
NP_055061                                                              15    9605
XP_006724446                                                           15    9605
XP_005244795                                                           15    9605
XP_006710612                                                           15    9605
XP_006710611                                                           15    9605
XP_006710610                                                           15    9605
XP_005244794                                                           15    9605
>NP_733751
          Length = 4911

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    MLSQERFLV 9
            M SQERFLV
Sbjct: 2492 MPSQERFLV 2500
>XP_006716142
          Length = 4927

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    MLSQERFLV 9
            M SQERFLV
Sbjct: 2509 MPSQERFLV 2517
>XP_005250088
          Length = 4928

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    MLSQERFLV 9
            M SQERFLV
Sbjct: 2509 MPSQERFLV 2517
>XP_006716141
          Length = 4959

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    MLSQERFLV 9
            M SQERFLV
Sbjct: 2509 MPSQERFLV 2517
>XP_005250085
          Length = 4979

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    MLSQERFLV 9
            M SQERFLV
Sbjct: 2509 MPSQERFLV 2517
>XP_006716140
          Length = 4982

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    MLSQERFLV 9
            M SQERFLV
Sbjct: 2509 MPSQERFLV 2517
>XP_005250084
          Length = 4982

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    MLSQERFLV 9
            M SQERFLV
Sbjct: 2509 MPSQERFLV 2517
>XP_005250083
          Length = 4982

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    MLSQERFLV 9
            M SQERFLV
Sbjct: 2508 MPSQERFLV 2516
>XP_005250082
          Length = 4983

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    MLSQERFLV 9
            M SQERFLV
Sbjct: 2509 MPSQERFLV 2517
>NP_001035521
          Length = 106

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2  LSQERFL 8
          +SQERFL
Sbjct: 79 ISQERFL 85
>NP_004930
          Length = 740

 Score = 16.2 bits (30), Expect =  5631
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2   LSQERFLV 9
           LSQ RFLV
Sbjct: 361 LSQVRFLV 368
>NP_001129628
          Length = 953

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2   LSQERFLV 9
           LSQ+RFL+
Sbjct: 409 LSQKRFLM 416
>NP_001254514
          Length = 1052

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2  LSQERFLV 9
          +SQ RFLV
Sbjct: 51 ISQRRFLV 58
>NP_001254515
          Length = 999

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2  LSQERFLV 9
          +SQ RFLV
Sbjct: 51 ISQRRFLV 58
>NP_001269469
          Length = 714

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2  LSQERFLV 9
          +SQ RFLV
Sbjct: 51 ISQRRFLV 58
>NP_060700
          Length = 1156

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1    MLSQERFLV 9
            M  QERFL+
Sbjct: 995  MAEQERFLL 1003
>NP_055725
          Length = 953

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2   LSQERFLV 9
           LSQ+RFL+
Sbjct: 409 LSQKRFLM 416
>XP_006721485
          Length = 805

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2  LSQERFLV 9
          +SQ RFLV
Sbjct: 51 ISQRRFLV 58
>XP_006721484
          Length = 997

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2  LSQERFLV 9
          +SQ RFLV
Sbjct: 51 ISQRRFLV 58
>XP_006721483
          Length = 1061

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2  LSQERFLV 9
          +SQ RFLV
Sbjct: 51 ISQRRFLV 58
>NP_006373
          Length = 752

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2  LSQERFLV 9
          +SQ RFLV
Sbjct: 51 ISQRRFLV 58
>NP_001141
          Length = 967

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   LSQERFLV 9
           LSQE FL+
Sbjct: 561 LSQEHFLL 568
>NP_065073
          Length = 2177

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1  MLSQERFL 8
          +LS+ERF+
Sbjct: 75 LLSEERFV 82
>XP_005254949
          Length = 967

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   LSQERFLV 9
           LSQE FL+
Sbjct: 561 LSQEHFLL 568
>XP_006715582
          Length = 2106

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1  MLSQERFL 8
          +LS+ERF+
Sbjct: 4  LLSEERFV 11
>NP_001123517
          Length = 673

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   MLSQERFLV 9
           +LSQ RF++
Sbjct: 556 LLSQRRFVL 564
>NP_001139627
          Length = 351

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   LSQERFLV 9
           +++ERFLV
Sbjct: 282 ITRERFLV 289
>NP_001139626
          Length = 351

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   LSQERFLV 9
           +++ERFLV
Sbjct: 282 ITRERFLV 289
>NP_689657
          Length = 351

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   LSQERFLV 9
           +++ERFLV
Sbjct: 282 ITRERFLV 289
>NP_055061
          Length = 3014

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2    LSQERFL 8
            +SQE+FL
Sbjct: 1214 MSQEKFL 1220
>XP_006724446
          Length = 3019

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2    LSQERFL 8
            +SQE+FL
Sbjct: 1214 MSQEKFL 1220
>XP_005244795
          Length = 600

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   MLSQERFLV 9
           +LSQ RF++
Sbjct: 483 LLSQRRFVL 491
>XP_006710612
          Length = 646

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   MLSQERFLV 9
           +LSQ RF++
Sbjct: 529 LLSQRRFVL 537
>XP_006710611
          Length = 673

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   MLSQERFLV 9
           +LSQ RF++
Sbjct: 556 LLSQRRFVL 564
>XP_006710610
          Length = 673

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   MLSQERFLV 9
           +LSQ RF++
Sbjct: 556 LLSQRRFVL 564
>XP_005244794
          Length = 673

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   MLSQERFLV 9
           +LSQ RF++
Sbjct: 556 LLSQRRFVL 564
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.339    0.144    0.360 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 421,436
Number of extensions: 345
Number of successful extensions: 36
Number of sequences better than 10000.0: 36
Number of HSP's gapped: 36
Number of HSP's successfully gapped: 36
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.5 bits)
S2: 28 (15.4 bits)

CANCERTOPE    |     Raghava's Group    |     IMTECH    |     CSIR    |     CRDD    |     GPSR Package