Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_001275701                                                           20     509
NP_004561                                                              20     509
NP_001275703                                                           20     509
XP_005253450                                                           20     509
XP_006719151                                                           20     509
NP_002901                                                              17    4312
NP_060264                                                              16    5631
XP_005251551                                                           16    5631
XP_006716857                                                           16    5631
NP_006258                                                              16    7355
NP_001157935                                                           16    7355
NP_477352                                                              16    7355
NP_001019628                                                           16    7355
NP_001116835                                                           16    7355
NP_005045                                                              16    7355
NP_001032955                                                           16    7355
NP_060392                                                              16    7355
NP_872394                                                              16    7355
NP_001071638                                                           16    7355
NP_001137485                                                           16    7355
NP_002051                                                              16    7355
XP_005261692                                                           16    7355
XP_005261691                                                           16    7355
XP_005263879                                                           16    7355
XP_005263878                                                           16    7355
XP_005263877                                                           16    7355
XP_005263876                                                           16    7355
XP_006712857                                                           16    7355
XP_005263813                                                           16    7355
XP_005263812                                                           16    7355
XP_005263810                                                           16    7355
XP_005263805                                                           16    7355
XP_005263804                                                           16    7355
XP_006712771                                                           16    7355
XP_005264064                                                           16    7355
XP_005264063                                                           16    7355
XP_005264062                                                           16    7355
XP_005264061                                                           16    7355
XP_005264060                                                           16    7355
XP_005264059                                                           16    7355
XP_006712517                                                           16    7355
XP_005264592                                                           16    7355
XP_005264371                                                           16    7355
XP_006712081                                                           16    7355
XP_005264370                                                           16    7355
XP_005264369                                                           16    7355
XP_005270807                                                           16    7355
NP_115636                                                              16    7355
NP_001186704                                                           15    9605
NP_001295                                                              15    9605
NP_005488                                                              15    9605
NP_060959                                                              15    9605
NP_068773                                                              15    9605
XP_005256978                                                           15    9605
XP_005256977                                                           15    9605
XP_005266410                                                           15    9605
XP_006711515                                                           15    9605
XP_006711514                                                           15    9605
XP_006711513                                                           15    9605
NP_001073852                                                           15    9605
NP_115991                                                              15    9605
>NP_001275701
          Length = 1486

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    MLLNLLEMM 9
            +LLNLLEMM
Sbjct: 1153 LLLNLLEMM 1161
>NP_004561
          Length = 1445

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    MLLNLLEMM 9
            +LLNLLEMM
Sbjct: 1112 LLLNLLEMM 1120
>NP_001275703
          Length = 1264

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   MLLNLLEMM 9
           +LLNLLEMM
Sbjct: 931 LLLNLLEMM 939
>XP_005253450
          Length = 1486

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    MLLNLLEMM 9
            +LLNLLEMM
Sbjct: 1153 LLLNLLEMM 1161
>XP_006719151
          Length = 1486

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    MLLNLLEMM 9
            +LLNLLEMM
Sbjct: 1153 LLLNLLEMM 1161
>NP_002901
          Length = 427

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   MLLNLLEMM 9
           MLLNL+E +
Sbjct: 194 MLLNLVEQL 202
>NP_060264
          Length = 1801

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   MLLNLLEMM 9
           + LNLLEM+
Sbjct: 380 LALNLLEMI 388
>XP_005251551
          Length = 1801

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   MLLNLLEMM 9
           + LNLLEM+
Sbjct: 380 LALNLLEMI 388
>XP_006716857
          Length = 1834

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   MLLNLLEMM 9
           + LNLLEM+
Sbjct: 413 LALNLLEMI 421
>NP_006258
          Length = 3224

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   NLLEMM 9
           NLLEMM
Sbjct: 341 NLLEMM 346
>NP_001157935
          Length = 1765

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   NLLEMM 9
           NLLEMM
Sbjct: 342 NLLEMM 347
>NP_477352
          Length = 2102

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   MLLNLLEM 8
           +L+NLLE+
Sbjct: 729 LLMNLLEL 736
>NP_001019628
          Length = 1748

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   NLLEMM 9
           NLLEMM
Sbjct: 333 NLLEMM 338
>NP_001116835
          Length = 1765

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   NLLEMM 9
           NLLEMM
Sbjct: 342 NLLEMM 347
>NP_005045
          Length = 1765

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   NLLEMM 9
           NLLEMM
Sbjct: 342 NLLEMM 347
>NP_001032955
          Length = 904

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   NLLEMM 9
           NLLEMM
Sbjct: 342 NLLEMM 347
>NP_060392
          Length = 669

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   MLLNLLEM 8
           + LNLLEM
Sbjct: 529 LYLNLLEM 536
>NP_872394
          Length = 1758

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   NLLEMM 9
           NLLEMM
Sbjct: 342 NLLEMM 347
>NP_001071638
          Length = 1756

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   NLLEMM 9
           NLLEMM
Sbjct: 341 NLLEMM 346
>NP_001137485
          Length = 1758

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   NLLEMM 9
           NLLEMM
Sbjct: 342 NLLEMM 347
>NP_002051
          Length = 333

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1   MLLNLLEMM 9
           ++LNLLE++
Sbjct: 221 LVLNLLELV 229
>XP_005261692
          Length = 1854

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   MLLNLLEM 8
           +L+NLLE+
Sbjct: 757 LLMNLLEL 764
>XP_005261691
          Length = 2099

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   MLLNLLEM 8
           +L+NLLE+
Sbjct: 757 LLMNLLEL 764
>XP_005263879
          Length = 1480

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4  NLLEMM 9
          NLLEMM
Sbjct: 57 NLLEMM 62
>XP_005263878
          Length = 1660

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   NLLEMM 9
           NLLEMM
Sbjct: 342 NLLEMM 347
>XP_005263877
          Length = 1686

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   NLLEMM 9
           NLLEMM
Sbjct: 342 NLLEMM 347
>XP_005263876
          Length = 1739

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   NLLEMM 9
           NLLEMM
Sbjct: 316 NLLEMM 321
>XP_006712857
          Length = 1748

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   NLLEMM 9
           NLLEMM
Sbjct: 325 NLLEMM 330
>XP_005263813
          Length = 1480

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4  NLLEMM 9
          NLLEMM
Sbjct: 57 NLLEMM 62
>XP_005263812
          Length = 1660

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   NLLEMM 9
           NLLEMM
Sbjct: 342 NLLEMM 347
>XP_005263810
          Length = 1739

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   NLLEMM 9
           NLLEMM
Sbjct: 316 NLLEMM 321
>XP_005263805
          Length = 1480

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4  NLLEMM 9
          NLLEMM
Sbjct: 57 NLLEMM 62
>XP_005263804
          Length = 1660

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   NLLEMM 9
           NLLEMM
Sbjct: 342 NLLEMM 347
>XP_006712771
          Length = 1766

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   NLLEMM 9
           NLLEMM
Sbjct: 342 NLLEMM 347
>XP_005264064
          Length = 2282

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   NLLEMM 9
           NLLEMM
Sbjct: 341 NLLEMM 346
>XP_005264063
          Length = 3136

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   NLLEMM 9
           NLLEMM
Sbjct: 341 NLLEMM 346
>XP_005264062
          Length = 3232

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   NLLEMM 9
           NLLEMM
Sbjct: 315 NLLEMM 320
>XP_005264061
          Length = 3232

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   NLLEMM 9
           NLLEMM
Sbjct: 341 NLLEMM 346
>XP_005264060
          Length = 3250

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   NLLEMM 9
           NLLEMM
Sbjct: 341 NLLEMM 346
>XP_005264059
          Length = 3258

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   NLLEMM 9
           NLLEMM
Sbjct: 341 NLLEMM 346
>XP_006712517
          Length = 1757

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   NLLEMM 9
           NLLEMM
Sbjct: 342 NLLEMM 347
>XP_005264592
          Length = 1653

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   NLLEMM 9
           NLLEMM
Sbjct: 341 NLLEMM 346
>XP_005264371
          Length = 1653

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   NLLEMM 9
           NLLEMM
Sbjct: 341 NLLEMM 346
>XP_006712081
          Length = 1679

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   NLLEMM 9
           NLLEMM
Sbjct: 341 NLLEMM 346
>XP_005264370
          Length = 1756

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   NLLEMM 9
           NLLEMM
Sbjct: 341 NLLEMM 346
>XP_005264369
          Length = 1756

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   NLLEMM 9
           NLLEMM
Sbjct: 341 NLLEMM 346
>XP_005270807
          Length = 333

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1   MLLNLLEMM 9
           ++LNLLE++
Sbjct: 221 LVLNLLELV 229
>NP_115636
          Length = 904

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   NLLEMM 9
           NLLEMM
Sbjct: 342 NLLEMM 347
>NP_001186704
          Length = 1133

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   MLLNLLEMM 9
           +LLNL+E +
Sbjct: 326 LLLNLIEYL 334
>NP_001295
          Length = 1380

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   MLLNLLEMM 9
           +LLNL+E +
Sbjct: 573 LLLNLIEYL 581
>NP_005488
          Length = 396

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   MLLNLLEMM 9
           +LLN+ EM+
Sbjct: 243 LLLNIWEML 251
>NP_060959
          Length = 2964

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1    MLLNLLE 7
            +LLNLLE
Sbjct: 2922 ILLNLLE 2928
>NP_068773
          Length = 435

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   MLLNLLEM 8
           +LLN+LE+
Sbjct: 215 LLLNMLEI 222
>XP_005256978
          Length = 396

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   MLLNLLEMM 9
           +LLN+ EM+
Sbjct: 243 LLLNIWEML 251
>XP_005256977
          Length = 396

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   MLLNLLEMM 9
           +LLN+ EM+
Sbjct: 243 LLLNIWEML 251
>XP_005266410
          Length = 435

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   MLLNLLEM 8
           +LLN+LE+
Sbjct: 215 LLLNMLEI 222
>XP_006711515
          Length = 2904

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1    MLLNLLE 7
            +LLNLLE
Sbjct: 2862 ILLNLLE 2868
>XP_006711514
          Length = 2964

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1    MLLNLLE 7
            +LLNLLE
Sbjct: 2922 ILLNLLE 2928
>XP_006711513
          Length = 2964

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1    MLLNLLE 7
            +LLNLLE
Sbjct: 2922 ILLNLLE 2928
>NP_001073852
          Length = 396

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   MLLNLLEMM 9
           +LLN+ EM+
Sbjct: 243 LLLNIWEML 251
>NP_115991
          Length = 543

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   MLLNLLEM 8
           +LLN+LE+
Sbjct: 223 LLLNILEI 230
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.343    0.148    0.365 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 365,611
Number of extensions: 599
Number of successful extensions: 61
Number of sequences better than 10000.0: 61
Number of HSP's gapped: 61
Number of HSP's successfully gapped: 61
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.6 bits)
S2: 28 (15.4 bits)

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