Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_001895                                                              20     390
NP_001091680                                                           20     390
NP_001091679                                                           20     390
XP_006713048                                                           20     390
XP_006713047                                                           20     390
XP_006713046                                                           20     390
XP_005264943                                                           20     390
NP_075252                                                              17    2528
NP_998812                                                              17    2528
NP_002002                                                              17    2528
XP_005265895                                                           17    2528
XP_005265894                                                           17    2528
NP_000375                                                              16    5631
NP_060764                                                              16    5631
NP_005088                                                              16    7355
NP_054644                                                              16    7355
NP_000784                                                              16    7355
NP_001007024                                                           16    7355
NP_001229431                                                           16    7355
NP_064450                                                              16    7355
NP_001229432                                                           16    7355
XP_005273257                                                           16    7355
NP_001431                                                              15    9605
XP_006716365                                                           15    9605
>NP_001895
          Length = 781

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   MLKHVVVNL 9
           MLKH VVNL
Sbjct: 131 MLKHAVVNL 139
>NP_001091680
          Length = 781

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   MLKHVVVNL 9
           MLKH VVNL
Sbjct: 131 MLKHAVVNL 139
>NP_001091679
          Length = 781

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   MLKHVVVNL 9
           MLKH VVNL
Sbjct: 131 MLKHAVVNL 139
>XP_006713048
          Length = 720

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   MLKHVVVNL 9
           MLKH VVNL
Sbjct: 131 MLKHAVVNL 139
>XP_006713047
          Length = 774

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   MLKHVVVNL 9
           MLKH VVNL
Sbjct: 124 MLKHAVVNL 132
>XP_006713046
          Length = 774

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   MLKHVVVNL 9
           MLKH VVNL
Sbjct: 124 MLKHAVVNL 132
>XP_005264943
          Length = 781

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   MLKHVVVNL 9
           MLKH VVNL
Sbjct: 131 MLKHAVVNL 139
>NP_075252
          Length = 762

 Score = 17.3 bits (33), Expect =  2528
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   LKHVVVN 8
           LKH+V+N
Sbjct: 284 LKHIVIN 290
>NP_998812
          Length = 802

 Score = 17.3 bits (33), Expect =  2528
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   LKHVVVN 8
           LKH+V+N
Sbjct: 284 LKHIVIN 290
>NP_002002
          Length = 802

 Score = 17.3 bits (33), Expect =  2528
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   LKHVVVN 8
           LKH+V+N
Sbjct: 284 LKHIVIN 290
>XP_005265895
          Length = 802

 Score = 17.3 bits (33), Expect =  2528
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   LKHVVVN 8
           LKH+V+N
Sbjct: 284 LKHIVIN 290
>XP_005265894
          Length = 834

 Score = 17.3 bits (33), Expect =  2528
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   LKHVVVN 8
           LKH+V+N
Sbjct: 316 LKHIVIN 322
>NP_000375
          Length = 4563

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2    LKHVVVNL 9
            +KH V+NL
Sbjct: 2317 VKHFVINL 2324
>NP_060764
          Length = 538

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   KHVVVNL 9
           KH+VVN+
Sbjct: 339 KHLVVNI 345
>NP_005088
          Length = 557

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   KHVVVNL 9
           KHV+V+L
Sbjct: 549 KHVIVDL 555
>NP_054644
          Length = 273

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1  MLKHVVVNL 9
          +LKHVV+ L
Sbjct: 33 LLKHVVLLL 41
>NP_000784
          Length = 273

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1  MLKHVVVNL 9
          +LKHVV+ L
Sbjct: 33 LLKHVVLLL 41
>NP_001007024
          Length = 309

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1  MLKHVVVNL 9
          +LKHVV+ L
Sbjct: 33 LLKHVVLLL 41
>NP_001229431
          Length = 146

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1  MLKHVVVNL 9
          +LKHVV+ L
Sbjct: 33 LLKHVVLLL 41
>NP_064450
          Length = 1722

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2    LKHVVVNL 9
            ++H VVNL
Sbjct: 1382 IQHAVVNL 1389
>NP_001229432
          Length = 110

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1  MLKHVVVNL 9
          +LKHVV+ L
Sbjct: 33 LLKHVVLLL 41
>XP_005273257
          Length = 1726

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2    LKHVVVNL 9
            ++H VVNL
Sbjct: 1386 IQHAVVNL 1393
>NP_001431
          Length = 919

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/6 (66%), Positives = 6/6 (100%)

Query: 4   HVVVNL 9
           HV++NL
Sbjct: 273 HVIINL 278
>XP_006716365
          Length = 919

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/6 (66%), Positives = 6/6 (100%)

Query: 4   HVVVNL 9
           HV++NL
Sbjct: 273 HVIINL 278
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.336    0.143    0.394 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 525,629
Number of extensions: 621
Number of successful extensions: 24
Number of sequences better than 10000.0: 24
Number of HSP's gapped: 24
Number of HSP's successfully gapped: 24
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.4 bits)
S2: 28 (15.4 bits)

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