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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_001275701                                                           19     869
NP_004561                                                              19     869
NP_001275703                                                           19     869
XP_005253450                                                           19     869
XP_006719151                                                           19     869
NP_001188357                                                           16    5631
NP_001139738                                                           16    5631
NP_056345                                                              16    5631
NP_001139739                                                           16    5631
NP_061843                                                              16    5631
NP_001139737                                                           16    5631
XP_005250508                                                           16    5631
XP_006712471                                                           16    5631
NP_996663                                                              15    9605

>NP_001275701
          Length = 1486

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    MLIVKSKTL 9
            MLIVKSKT+
Sbjct: 1451 MLIVKSKTV 1459



>NP_004561
          Length = 1445

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    MLIVKSKTL 9
            MLIVKSKT+
Sbjct: 1410 MLIVKSKTV 1418



>NP_001275703
          Length = 1264

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    MLIVKSKTL 9
            MLIVKSKT+
Sbjct: 1229 MLIVKSKTV 1237



>XP_005253450
          Length = 1486

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    MLIVKSKTL 9
            MLIVKSKT+
Sbjct: 1451 MLIVKSKTV 1459



>XP_006719151
          Length = 1486

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    MLIVKSKTL 9
            MLIVKSKT+
Sbjct: 1451 MLIVKSKTV 1459



>NP_001188357
          Length = 384

 Score = 16.2 bits (30), Expect =  5631
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1 MLIVKSKTL 9
         MLI  SKTL
Sbjct: 1 MLIYSSKTL 9



>NP_001139738
          Length = 370

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1  MLIVKSKTL 9
          +L+VK KTL
Sbjct: 43 ILLVKDKTL 51



>NP_056345
          Length = 452

 Score = 16.2 bits (30), Expect =  5631
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1  MLIVKSKTL 9
          MLI  SKTL
Sbjct: 69 MLIYSSKTL 77



>NP_001139739
          Length = 370

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1  MLIVKSKTL 9
          +L+VK KTL
Sbjct: 43 ILLVKDKTL 51



>NP_061843
          Length = 370

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1  MLIVKSKTL 9
          +L+VK KTL
Sbjct: 43 ILLVKDKTL 51



>NP_001139737
          Length = 370

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1  MLIVKSKTL 9
          +L+VK KTL
Sbjct: 43 ILLVKDKTL 51



>XP_005250508
          Length = 370

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1  MLIVKSKTL 9
          +L+VK KTL
Sbjct: 43 ILLVKDKTL 51



>XP_006712471
          Length = 384

 Score = 16.2 bits (30), Expect =  5631
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1 MLIVKSKTL 9
         MLI  SKTL
Sbjct: 1 MLIYSSKTL 9



>NP_996663
          Length = 262

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   LIVKSKTL 9
           LIVK KT+
Sbjct: 210 LIVKDKTV 217



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.326    0.135    0.308 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 274,974
Number of extensions: 243
Number of successful extensions: 14
Number of sequences better than 10000.0: 14
Number of HSP's gapped: 14
Number of HSP's successfully gapped: 14
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.1 bits)
S2: 28 (15.4 bits)