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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_006209                                                              20     509
XP_006713721                                                           20     509
NP_976059                                                              17    3301
NP_976060                                                              17    3301
NP_004042                                                              17    3301
XP_005269412                                                           17    3301
XP_005269409                                                           17    3301
NP_055955                                                              16    5631
NP_002664                                                              16    7355
NP_001123554                                                           16    7355
NP_001034464                                                           16    7355
NP_077025                                                              16    7355
XP_006723687                                                           16    7355
XP_006723686                                                           16    7355
XP_006723685                                                           16    7355
XP_005250117                                                           16    7355
XP_005265291                                                           16    7355
XP_006713269                                                           16    7355
NP_003583                                                              16    7355
NP_001026                                                              15    9605
NP_059113                                                              15    9605
NP_149975                                                              15    9605
XP_006711873                                                           15    9605
XP_006711872                                                           15    9605
XP_006711871                                                           15    9605
XP_006711870                                                           15    9605
XP_006711869                                                           15    9605
XP_006711868                                                           15    9605
XP_006711867                                                           15    9605
XP_006711866                                                           15    9605
XP_006711865                                                           15    9605

>NP_006209
          Length = 1068

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   MLGRMSNLM 9
           MLGRM NLM
Sbjct: 278 MLGRMPNLM 286



>XP_006713721
          Length = 1068

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   MLGRMSNLM 9
           MLGRM NLM
Sbjct: 278 MLGRMPNLM 286



>NP_976059
          Length = 343

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   MLGRMSN 7
           MLGRM+N
Sbjct: 196 MLGRMAN 202



>NP_976060
          Length = 343

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   MLGRMSN 7
           MLGRM+N
Sbjct: 196 MLGRMAN 202



>NP_004042
          Length = 343

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   MLGRMSN 7
           MLGRM+N
Sbjct: 196 MLGRMAN 202



>XP_005269412
          Length = 206

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1  MLGRMSN 7
          MLGRM+N
Sbjct: 59 MLGRMAN 65



>XP_005269409
          Length = 343

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   MLGRMSN 7
           MLGRM+N
Sbjct: 196 MLGRMAN 202



>NP_055955
          Length = 644

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   MLGRMSNLM 9
           +L RM+NLM
Sbjct: 141 LLHRMANLM 149



>NP_002664
          Length = 2135

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2    LGRMSNLM 9
            LG++SNL+
Sbjct: 1603 LGQLSNLL 1610



>NP_001123554
          Length = 2135

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2    LGRMSNLM 9
            LG++SNL+
Sbjct: 1603 LGQLSNLL 1610



>NP_001034464
          Length = 317

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   LGRMSNLM 9
           LG+++NLM
Sbjct: 303 LGKINNLM 310



>NP_077025
          Length = 345

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   LGRMSNLM 9
           LG+++NLM
Sbjct: 331 LGKINNLM 338



>XP_006723687
          Length = 188

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   LGRMSNLM 9
           LG+++NLM
Sbjct: 174 LGKINNLM 181



>XP_006723686
          Length = 188

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   LGRMSNLM 9
           LG+++NLM
Sbjct: 174 LGKINNLM 181



>XP_006723685
          Length = 188

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   LGRMSNLM 9
           LG+++NLM
Sbjct: 174 LGKINNLM 181



>XP_005250117
          Length = 776

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   LGRMSNLM 9
           LGRM NL+
Sbjct: 319 LGRMYNLV 326



>XP_005265291
          Length = 1211

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   LGRMSNLM 9
           LG++SNL+
Sbjct: 679 LGQLSNLL 686



>XP_006713269
          Length = 2052

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2    LGRMSNLM 9
            LG++SNL+
Sbjct: 1520 LGQLSNLL 1527



>NP_003583
          Length = 776

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   LGRMSNLM 9
           LGRM NL+
Sbjct: 319 LGRMYNLV 326



>NP_001026
          Length = 4967

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2    LGRMSNLM 9
            LGR+ N+M
Sbjct: 1557 LGRIKNVM 1564



>NP_059113
          Length = 637

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   GRMSNLM 9
           GRM NL+
Sbjct: 498 GRMKNLL 504



>NP_149975
          Length = 555

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 9/9 (100%)

Query: 1   MLGRMSNLM 9
           +LG++SN++
Sbjct: 448 LLGKISNIV 456



>XP_006711873
          Length = 4954

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2    LGRMSNLM 9
            LGR+ N+M
Sbjct: 1556 LGRIKNVM 1563



>XP_006711872
          Length = 2735

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2    LGRMSNLM 9
            LGR+ N+M
Sbjct: 1567 LGRIKNVM 1574



>XP_006711871
          Length = 4906

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2    LGRMSNLM 9
            LGR+ N+M
Sbjct: 1567 LGRIKNVM 1574



>XP_006711870
          Length = 4965

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2    LGRMSNLM 9
            LGR+ N+M
Sbjct: 1567 LGRIKNVM 1574



>XP_006711869
          Length = 4973

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2    LGRMSNLM 9
            LGR+ N+M
Sbjct: 1567 LGRIKNVM 1574



>XP_006711868
          Length = 4975

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2    LGRMSNLM 9
            LGR+ N+M
Sbjct: 1557 LGRIKNVM 1564



>XP_006711867
          Length = 4977

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2    LGRMSNLM 9
            LGR+ N+M
Sbjct: 1567 LGRIKNVM 1574



>XP_006711866
          Length = 4984

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2    LGRMSNLM 9
            LGR+ N+M
Sbjct: 1566 LGRIKNVM 1573



>XP_006711865
          Length = 4985

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2    LGRMSNLM 9
            LGR+ N+M
Sbjct: 1567 LGRIKNVM 1574



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.336    0.137    0.368 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 383,882
Number of extensions: 477
Number of successful extensions: 31
Number of sequences better than 10000.0: 31
Number of HSP's gapped: 31
Number of HSP's successfully gapped: 31
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.4 bits)
S2: 28 (15.4 bits)