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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_899243                                                              18    1482
NP_001034842                                                           18    1482
XP_006720135                                                           18    1482
XP_006720134                                                           18    1482
XP_006720133                                                           18    1482
XP_006712079                                                           18    1482
NP_001229886                                                           16    5631
NP_001229314                                                           16    5631
NP_005897                                                              16    5631
XP_006715161                                                           16    5631
NP_150229                                                              16    7355
NP_004960                                                              15    9605
NP_066357                                                              15    9605
NP_001159418                                                           15    9605
NP_000992                                                              15    9605
NP_001186902                                                           15    9605
XP_006726405                                                           15    9605
XP_005269823                                                           15    9605
XP_006711156                                                           15    9605

>NP_899243
          Length = 1977

 Score = 18.1 bits (35), Expect =  1482
 Identities = 6/9 (66%), Positives = 9/9 (100%)

Query: 1    MLAIRRMKK 9
            MLA+R++KK
Sbjct: 1033 MLAVRKLKK 1041



>NP_001034842
          Length = 1958

 Score = 18.1 bits (35), Expect =  1482
 Identities = 6/9 (66%), Positives = 9/9 (100%)

Query: 1    MLAIRRMKK 9
            MLA+R++KK
Sbjct: 1033 MLAVRKLKK 1041



>XP_006720135
          Length = 1454

 Score = 18.1 bits (35), Expect =  1482
 Identities = 6/9 (66%), Positives = 9/9 (100%)

Query: 1    MLAIRRMKK 9
            MLA+R++KK
Sbjct: 1033 MLAVRKLKK 1041



>XP_006720134
          Length = 1579

 Score = 18.1 bits (35), Expect =  1482
 Identities = 6/9 (66%), Positives = 9/9 (100%)

Query: 1    MLAIRRMKK 9
            MLA+R++KK
Sbjct: 1033 MLAVRKLKK 1041



>XP_006720133
          Length = 1969

 Score = 18.1 bits (35), Expect =  1482
 Identities = 6/9 (66%), Positives = 9/9 (100%)

Query: 1    MLAIRRMKK 9
            MLA+R++KK
Sbjct: 1033 MLAVRKLKK 1041



>XP_006712079
          Length = 1683

 Score = 18.1 bits (35), Expect =  1482
 Identities = 6/9 (66%), Positives = 9/9 (100%)

Query: 1   MLAIRRMKK 9
           MLA+R++KK
Sbjct: 791 MLAVRKLKK 799



>NP_001229886
          Length = 648

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1  MLAIRRMKK 9
          ++AI+RMK+
Sbjct: 29 LVAIKRMKR 37



>NP_001229314
          Length = 583

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1  MLAIRRMKK 9
          ++AI+RMK+
Sbjct: 29 LVAIKRMKR 37



>NP_005897
          Length = 623

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1  MLAIRRMKK 9
          ++AI+RMK+
Sbjct: 29 LVAIKRMKR 37



>XP_006715161
          Length = 490

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1  MLAIRRMKK 9
          ++AI+RMK+
Sbjct: 29 LVAIKRMKR 37



>NP_150229
          Length = 1359

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   LAIRRMKK 9
           +++RRMKK
Sbjct: 406 VSLRRMKK 413



>NP_004960
          Length = 1019

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   LAIRRMKK 9
           LAIRR+ K
Sbjct: 889 LAIRRLDK 896



>NP_066357
          Length = 142

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   MLAIRRMK 8
           MLAI+R K
Sbjct: 118 MLAIKRCK 125



>NP_001159418
          Length = 464

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   LAIRRMKK 9
           LAIRR+ K
Sbjct: 334 LAIRRLDK 341



>NP_000992
          Length = 106

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1  MLAIRRMK 8
          MLAI+R K
Sbjct: 82 MLAIKRCK 89



>NP_001186902
          Length = 154

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   MLAIRRMK 8
           MLAI+R K
Sbjct: 118 MLAIKRCK 125



>XP_006726405
          Length = 1318

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1  MLAIRRMKK 9
          M  +RRM+K
Sbjct: 50 MFKLRRMRK 58



>XP_005269823
          Length = 1019

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   LAIRRMKK 9
           LAIRR+ K
Sbjct: 889 LAIRRLDK 896



>XP_006711156
          Length = 1318

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1  MLAIRRMKK 9
          M  +RRM+K
Sbjct: 50 MFKLRRMRK 58



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.348    0.143    0.356 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 319,212
Number of extensions: 429
Number of successful extensions: 19
Number of sequences better than 10000.0: 19
Number of HSP's gapped: 19
Number of HSP's successfully gapped: 19
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 14 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.9 bits)
S2: 28 (15.4 bits)