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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_001245210                                                           19     665
NP_000242                                                              19     665
XP_005264389                                                           19     665
NP_006217                                                              17    3301
XP_005246701                                                           17    3301
XP_005246700                                                           17    3301
NP_710159                                                              17    4312
XP_005273485                                                           17    4312
NP_001713                                                              16    7355
NP_524144                                                              15    9605

>NP_001245210
          Length = 868

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   MKLDIAAVK 9
           MKLDIAAV+
Sbjct: 234 MKLDIAAVR 242



>NP_000242
          Length = 934

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   MKLDIAAVK 9
           MKLDIAAV+
Sbjct: 300 MKLDIAAVR 308



>XP_005264389
          Length = 921

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   MKLDIAAVK 9
           MKLDIAAV+
Sbjct: 300 MKLDIAAVR 308



>NP_006217
          Length = 1095

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2   KLDIAAVK 9
           KLDI+A+K
Sbjct: 157 KLDISAIK 164



>XP_005246701
          Length = 1016

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2  KLDIAAVK 9
          KLDI+A+K
Sbjct: 78 KLDISAIK 85



>XP_005246700
          Length = 1021

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2  KLDIAAVK 9
          KLDI+A+K
Sbjct: 83 KLDISAIK 90



>NP_710159
          Length = 476

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   MKLDIAAVK 9
           +KLD+A +K
Sbjct: 245 LKLDLAVIK 253



>XP_005273485
          Length = 464

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   MKLDIAAVK 9
           +KLD+A +K
Sbjct: 233 LKLDLAVIK 241



>NP_001713
          Length = 398

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   MKLDIAAVK 9
           M++DI AVK
Sbjct: 210 MEVDIPAVK 218



>NP_524144
          Length = 194

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 2  KLDIAAVK 9
          K+D++A+K
Sbjct: 37 KIDLSAIK 44



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.326    0.134    0.323 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 313,035
Number of extensions: 149
Number of successful extensions: 10
Number of sequences better than 10000.0: 10
Number of HSP's gapped: 10
Number of HSP's successfully gapped: 10
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.1 bits)
S2: 28 (15.4 bits)