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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_115927                                                              18    1135
NP_112593                                                              16    5631
XP_006722617                                                           16    5631
XP_005258414                                                           16    5631
NP_056138                                                              15    9605

>NP_115927
          Length = 1321

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   MKKSSVTAE 9
           MKKS VTAE
Sbjct: 844 MKKSPVTAE 852



>NP_112593
          Length = 466

 Score = 16.2 bits (30), Expect =  5631
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1  MKKSSVTAE 9
          +KKSS TAE
Sbjct: 18 VKKSSDTAE 26



>XP_006722617
          Length = 538

 Score = 16.2 bits (30), Expect =  5631
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1  MKKSSVTAE 9
          +KKSS TAE
Sbjct: 90 VKKSSDTAE 98



>XP_005258414
          Length = 564

 Score = 16.2 bits (30), Expect =  5631
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   MKKSSVTAE 9
           +KKSS TAE
Sbjct: 116 VKKSSDTAE 124



>NP_056138
          Length = 794

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   KKSSVTAE 9
           +KSSVT E
Sbjct: 787 RKSSVTEE 794



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.285   0.0942    0.199 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 289,524
Number of extensions: 266
Number of successful extensions: 5
Number of sequences better than 10000.0: 5
Number of HSP's gapped: 5
Number of HSP's successfully gapped: 5
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 18 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (14.9 bits)
S2: 28 (15.4 bits)