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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_065928                                                              18    1935
XP_005256527                                                           18    1935
NP_955452                                                              17    2528
XP_006713543                                                           17    2528
NP_741996                                                              17    3301
NP_001269828                                                           16    5631
NP_001269829                                                           16    5631
NP_001269830                                                           16    5631
NP_001093867                                                           16    5631
NP_001092882                                                           16    5631
NP_006757                                                              16    5631
NP_008954                                                              16    5631
NP_001092883                                                           16    5631
XP_006716734                                                           16    5631
XP_006716732                                                           16    5631
XP_006716731                                                           16    5631
XP_006716730                                                           16    5631
XP_005273706                                                           16    5631
XP_006712269                                                           16    5631
XP_006712268                                                           16    5631
XP_005246303                                                           16    5631
NP_006737                                                              16    7355
NP_001153101                                                           16    7355
NP_003636                                                              16    7355
NP_001032624                                                           16    7355
NP_001258349                                                           16    7355
NP_001032625                                                           16    7355
NP_060245                                                              16    7355
NP_001032629                                                           16    7355
XP_005274636                                                           16    7355
XP_006724572                                                           16    7355
XP_006724571                                                           16    7355
XP_005274635                                                           16    7355
NP_001108624                                                           15    9605
NP_620061                                                              15    9605

>NP_065928
          Length = 4427

 Score = 17.7 bits (34), Expect =  1935
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1    MKKNEISI 8
            MKKN++SI
Sbjct: 1682 MKKNQVSI 1689



>XP_005256527
          Length = 4427

 Score = 17.7 bits (34), Expect =  1935
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1    MKKNEISI 8
            MKKN++SI
Sbjct: 1682 MKKNQVSI 1689



>NP_955452
          Length = 2590

 Score = 17.3 bits (33), Expect =  2528
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1    MKKNEISI 8
            +KKNE+S+
Sbjct: 1430 LKKNEVSV 1437



>XP_006713543
          Length = 2591

 Score = 17.3 bits (33), Expect =  2528
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1    MKKNEISI 8
            +KKNE+S+
Sbjct: 1431 LKKNEVSV 1438



>NP_741996
          Length = 1300

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    MKKNEISI 8
            MK NEIS+
Sbjct: 1225 MKNNEISV 1232



>NP_001269828
          Length = 858

 Score = 16.2 bits (30), Expect =  5631
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1   MKKNEISIF 9
           +K N++S+F
Sbjct: 570 LKPNDVSVF 578



>NP_001269829
          Length = 840

 Score = 16.2 bits (30), Expect =  5631
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1   MKKNEISIF 9
           +K N++S+F
Sbjct: 552 LKPNDVSVF 560



>NP_001269830
          Length = 791

 Score = 16.2 bits (30), Expect =  5631
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1   MKKNEISIF 9
           +K N++S+F
Sbjct: 503 LKPNDVSVF 511



>NP_001093867
          Length = 867

 Score = 16.2 bits (30), Expect =  5631
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1   MKKNEISIF 9
           +K N++S+F
Sbjct: 579 LKPNDVSVF 587



>NP_001092882
          Length = 2004

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   KKNEISIF 9
           +KN IS+F
Sbjct: 572 RKNNISVF 579



>NP_006757
          Length = 2004

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   KKNEISIF 9
           +KN IS+F
Sbjct: 572 RKNNISVF 579



>NP_008954
          Length = 1011

 Score = 16.2 bits (30), Expect =  5631
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1   MKKNEISIF 9
           +K N++S+F
Sbjct: 723 LKPNDVSVF 731



>NP_001092883
          Length = 2004

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   KKNEISIF 9
           +KN IS+F
Sbjct: 572 RKNNISVF 579



>XP_006716734
          Length = 264

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2  KKNEISIF 9
          KKNE+S F
Sbjct: 18 KKNELSNF 25



>XP_006716732
          Length = 300

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2  KKNEISIF 9
          KKNE+S F
Sbjct: 18 KKNELSNF 25



>XP_006716731
          Length = 300

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2  KKNEISIF 9
          KKNE+S F
Sbjct: 18 KKNELSNF 25



>XP_006716730
          Length = 300

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2  KKNEISIF 9
          KKNE+S F
Sbjct: 18 KKNELSNF 25



>XP_005273706
          Length = 1997

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   KKNEISIF 9
           +KN IS+F
Sbjct: 565 RKNNISVF 572



>XP_006712269
          Length = 838

 Score = 16.2 bits (30), Expect =  5631
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1   MKKNEISIF 9
           +K N++S+F
Sbjct: 550 LKPNDVSVF 558



>XP_006712268
          Length = 840

 Score = 16.2 bits (30), Expect =  5631
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1   MKKNEISIF 9
           +K N++S+F
Sbjct: 552 LKPNDVSVF 560



>XP_005246303
          Length = 993

 Score = 16.2 bits (30), Expect =  5631
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1   MKKNEISIF 9
           +K N++S+F
Sbjct: 705 LKPNDVSVF 713



>NP_006737
          Length = 302

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   MKKNEI 6
           MKKNE+
Sbjct: 95  MKKNEV 100



>NP_001153101
          Length = 567

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/9 (44%), Positives = 9/9 (100%)

Query: 1   MKKNEISIF 9
           +KK+++SI+
Sbjct: 172 LKKDDVSIY 180



>NP_003636
          Length = 620

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/9 (44%), Positives = 9/9 (100%)

Query: 1   MKKNEISIF 9
           +KK+++SI+
Sbjct: 172 LKKDDVSIY 180



>NP_001032624
          Length = 208

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1 MKKNEI 6
         MKKNE+
Sbjct: 1 MKKNEV 6



>NP_001258349
          Length = 273

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   MKKNEISI 8
           +KK+EIS+
Sbjct: 243 LKKDEISV 250



>NP_001032625
          Length = 208

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1 MKKNEI 6
         MKKNE+
Sbjct: 1 MKKNEV 6



>NP_060245
          Length = 380

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   MKKNEISI 8
           +KK+EIS+
Sbjct: 350 LKKDEISV 357



>NP_001032629
          Length = 329

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   MKKNEI 6
           MKKNE+
Sbjct: 122 MKKNEV 127



>XP_005274636
          Length = 301

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1  MKKNEI 6
          MKKNE+
Sbjct: 94 MKKNEV 99



>XP_006724572
          Length = 301

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1  MKKNEI 6
          MKKNE+
Sbjct: 94 MKKNEV 99



>XP_006724571
          Length = 329

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   MKKNEI 6
           MKKNE+
Sbjct: 122 MKKNEV 127



>XP_005274635
          Length = 330

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   MKKNEI 6
           MKKNE+
Sbjct: 123 MKKNEV 128



>NP_001108624
          Length = 194

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1  MKKNEISI 8
          +KKN +SI
Sbjct: 74 VKKNRVSI 81



>NP_620061
          Length = 417

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1  MKKNEIS 7
          MKKN+I+
Sbjct: 29 MKKNQIT 35



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.322    0.135    0.339 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 452,310
Number of extensions: 583
Number of successful extensions: 35
Number of sequences better than 10000.0: 35
Number of HSP's gapped: 35
Number of HSP's successfully gapped: 35
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.9 bits)
S2: 28 (15.4 bits)