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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_055365                                                              18    1135
NP_001258358                                                           17    3301
NP_001258357                                                           17    3301
NP_055862                                                              17    3301
NP_001258356                                                           17    3301
XP_005245845                                                           17    3301
XP_005245844                                                           17    3301
NP_001258547                                                           16    7355
NP_060077                                                              16    7355
XP_005259070                                                           16    7355

>NP_055365
          Length = 1032

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   MITMRLAQL 9
           MI MRLAQL
Sbjct: 208 MIAMRLAQL 216



>NP_001258358
          Length = 971

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   MITMRLAQL 9
           MIT RLAQ+
Sbjct: 955 MITKRLAQV 963



>NP_001258357
          Length = 992

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   MITMRLAQL 9
           MIT RLAQ+
Sbjct: 976 MITKRLAQV 984



>NP_055862
          Length = 993

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   MITMRLAQL 9
           MIT RLAQ+
Sbjct: 977 MITKRLAQV 985



>NP_001258356
          Length = 992

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   MITMRLAQL 9
           MIT RLAQ+
Sbjct: 976 MITKRLAQV 984



>XP_005245845
          Length = 995

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   MITMRLAQL 9
           MIT RLAQ+
Sbjct: 979 MITKRLAQV 987



>XP_005245844
          Length = 996

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   MITMRLAQL 9
           MIT RLAQ+
Sbjct: 980 MITKRLAQV 988



>NP_001258547
          Length = 780

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   TMRLAQL 9
           T+RLAQL
Sbjct: 703 TLRLAQL 709



>NP_060077
          Length = 782

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   TMRLAQL 9
           T+RLAQL
Sbjct: 705 TLRLAQL 711



>XP_005259070
          Length = 781

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   TMRLAQL 9
           T+RLAQL
Sbjct: 704 TLRLAQL 710



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.349    0.142    0.346 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 200,101
Number of extensions: 123
Number of successful extensions: 10
Number of sequences better than 10000.0: 10
Number of HSP's gapped: 10
Number of HSP's successfully gapped: 10
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 14 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.9 bits)
S2: 28 (15.4 bits)