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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_733751                                                              18    1135
XP_006716142                                                           18    1135
XP_005250088                                                           18    1135
XP_006716141                                                           18    1135
XP_005250085                                                           18    1135
XP_006716140                                                           18    1135
XP_005250084                                                           18    1135
XP_005250083                                                           18    1135
XP_005250082                                                           18    1135
NP_001929                                                              16    5631
NP_001349                                                              16    7355
NP_001253969                                                           15    9605
NP_004964                                                              15    9605
NP_066554                                                              15    9605
NP_001242953                                                           15    9605
NP_067049                                                              15    9605
NP_001243404                                                           15    9605
NP_001242955                                                           15    9605
NP_001242954                                                           15    9605
XP_005261676                                                           15    9605
XP_006724320                                                           15    9605
XP_006717997                                                           15    9605
XP_005270071                                                           15    9605
XP_005250695                                                           15    9605
XP_005249146                                                           15    9605
NP_115540                                                              15    9605

>NP_733751
          Length = 4911

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    MIRQRSELF 9
            MIRQRSE F
Sbjct: 3075 MIRQRSEPF 3083



>XP_006716142
          Length = 4927

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    MIRQRSELF 9
            MIRQRSE F
Sbjct: 3092 MIRQRSEPF 3100



>XP_005250088
          Length = 4928

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    MIRQRSELF 9
            MIRQRSE F
Sbjct: 3092 MIRQRSEPF 3100



>XP_006716141
          Length = 4959

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    MIRQRSELF 9
            MIRQRSE F
Sbjct: 3092 MIRQRSEPF 3100



>XP_005250085
          Length = 4979

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    MIRQRSELF 9
            MIRQRSE F
Sbjct: 3092 MIRQRSEPF 3100



>XP_006716140
          Length = 4982

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    MIRQRSELF 9
            MIRQRSE F
Sbjct: 3092 MIRQRSEPF 3100



>XP_005250084
          Length = 4982

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    MIRQRSELF 9
            MIRQRSE F
Sbjct: 3092 MIRQRSEPF 3100



>XP_005250083
          Length = 4982

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    MIRQRSELF 9
            MIRQRSE F
Sbjct: 3091 MIRQRSEPF 3099



>XP_005250082
          Length = 4983

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    MIRQRSELF 9
            MIRQRSE F
Sbjct: 3092 MIRQRSEPF 3100



>NP_001929
          Length = 176

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   MIRQRSELF 9
           ++RQ+ ELF
Sbjct: 118 LLRQQQELF 126



>NP_001349
          Length = 795

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   MIRQRSEL 8
           ++RQRS+L
Sbjct: 279 VVRQRSDL 286



>NP_001253969
          Length = 1074

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   IRQRSELF 9
           +RQR +LF
Sbjct: 921 LRQRRQLF 928



>NP_004964
          Length = 1246

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2    IRQRSELF 9
            +RQR +LF
Sbjct: 1093 LRQRRQLF 1100



>NP_066554
          Length = 1020

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   RQRSEL 8
           RQRSEL
Sbjct: 344 RQRSEL 349



>NP_001242953
          Length = 714

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   MIRQRSELF 9
           +IR+ S+LF
Sbjct: 357 LIRKHSQLF 365



>NP_067049
          Length = 698

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   MIRQRSELF 9
           +IR+ S+LF
Sbjct: 341 LIRKHSQLF 349



>NP_001243404
          Length = 465

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   RQRSEL 8
           RQRSEL
Sbjct: 360 RQRSEL 365



>NP_001242955
          Length = 608

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   MIRQRSELF 9
           +IR+ S+LF
Sbjct: 251 LIRKHSQLF 259



>NP_001242954
          Length = 704

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   MIRQRSELF 9
           +IR+ S+LF
Sbjct: 347 LIRKHSQLF 355



>XP_005261676
          Length = 986

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   RQRSEL 8
           RQRSEL
Sbjct: 344 RQRSEL 349



>XP_006724320
          Length = 1006

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   RQRSEL 8
           RQRSEL
Sbjct: 344 RQRSEL 349



>XP_006717997
          Length = 608

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   MIRQRSELF 9
           +IR+ S+LF
Sbjct: 251 LIRKHSQLF 259



>XP_005270071
          Length = 624

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   MIRQRSELF 9
           +IR+ S+LF
Sbjct: 267 LIRKHSQLF 275



>XP_005250695
          Length = 354

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   RQRSELF 9
           +QRS+LF
Sbjct: 161 KQRSDLF 167




 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   RQRSELF 9
           +QRS+LF
Sbjct: 272 KQRSDLF 278



>XP_005249146
          Length = 1074

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   IRQRSELF 9
           +RQR +LF
Sbjct: 921 LRQRRQLF 928



>NP_115540
          Length = 561

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   RQRSELF 9
           +QRS+LF
Sbjct: 368 KQRSDLF 374




 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   RQRSELF 9
           +QRS+LF
Sbjct: 479 KQRSDLF 485



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.343    0.146    0.376 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 374,191
Number of extensions: 510
Number of successful extensions: 28
Number of sequences better than 10000.0: 26
Number of HSP's gapped: 28
Number of HSP's successfully gapped: 28
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.7 bits)
S2: 28 (15.4 bits)