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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_112503                                                              16    7355
NP_000094                                                              16    7355
NP_004555                                                              16    7355
XP_006720469                                                           16    7355
XP_005254249                                                           16    7355
XP_005254248                                                           16    7355
XP_005254247                                                           16    7355
XP_005263120                                                           16    7355
NP_001124438                                                           15    9605
NP_001159726                                                           15    9605
NP_001159725                                                           15    9605
NP_001052                                                              15    9605
NP_112246                                                              15    9605

>NP_112503
          Length = 503

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   MIMIKEVLV 9
           M+M+K +LV
Sbjct: 444 MVMMKAILV 452



>NP_000094
          Length = 503

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   MIMIKEVLV 9
           M+M+K +LV
Sbjct: 444 MVMMKAILV 452



>NP_004555
          Length = 557

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   IMIKEVL 8
           +MIKE+L
Sbjct: 545 VMIKEIL 551



>XP_006720469
          Length = 401

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   MIMIKEVLV 9
           M+M+K +LV
Sbjct: 342 MVMMKAILV 350



>XP_005254249
          Length = 444

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   MIMIKEVLV 9
           M+M+K +LV
Sbjct: 385 MVMMKAILV 393



>XP_005254248
          Length = 503

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   MIMIKEVLV 9
           M+M+K +LV
Sbjct: 444 MVMMKAILV 452



>XP_005254247
          Length = 503

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   MIMIKEVLV 9
           M+M+K +LV
Sbjct: 444 MVMMKAILV 452



>XP_005263120
          Length = 512

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   IMIKEVL 8
           +MIKE+L
Sbjct: 500 VMIKEIL 506



>NP_001124438
          Length = 534

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   MIKEVLV 9
           MIK+VLV
Sbjct: 96  MIKQVLV 102



>NP_001159726
          Length = 466

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3  MIKEVLV 9
          MIK+VLV
Sbjct: 28 MIKQVLV 34



>NP_001159725
          Length = 580

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   MIKEVLV 9
           MIK+VLV
Sbjct: 96  MIKQVLV 102



>NP_001052
          Length = 534

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   MIKEVLV 9
           MIK+VLV
Sbjct: 96  MIKQVLV 102



>NP_112246
          Length = 460

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   MIKEVLV 9
           MIK+VLV
Sbjct: 96  MIKQVLV 102



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.344    0.154    0.358 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 308,119
Number of extensions: 221
Number of successful extensions: 13
Number of sequences better than 10000.0: 13
Number of HSP's gapped: 13
Number of HSP's successfully gapped: 13
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.5 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.6 bits)
S2: 28 (15.4 bits)