Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_001239058                                                           16    5631
NP_036453                                                              16    5631
NP_001014431                                                           16    7355
NP_005154                                                              16    7355
NP_001014432                                                           16    7355
NP_004906                                                              16    7355
NP_997511                                                              16    7355
NP_058198                                                              16    7355
NP_997512                                                              16    7355
NP_997510                                                              16    7355
NP_997057                                                              16    7355
XP_005267458                                                           16    7355
XP_005262788                                                           16    7355
NP_001095940                                                           16    7355
NP_000663                                                              16    7355
NP_001124438                                                           15    9605
NP_001159726                                                           15    9605
NP_001159725                                                           15    9605
NP_001052                                                              15    9605
NP_112246                                                              15    9605
NP_001193658                                                           15    9605
NP_859029                                                              15    9605
NP_005456                                                              15    9605
NP_001617                                                              15    9605
NP_001229957                                                           15    9605
NP_001229956                                                           15    9605
NP_004555                                                              15    9605
XP_006725026                                                           15    9605
XP_006725025                                                           15    9605
XP_006725024                                                           15    9605
XP_006725023                                                           15    9605
XP_006725022                                                           15    9605
XP_006723148                                                           15    9605
XP_006723147                                                           15    9605
XP_006723146                                                           15    9605
XP_006723145                                                           15    9605
XP_006723144                                                           15    9605
XP_006719480                                                           15    9605
XP_006719479                                                           15    9605
XP_006719478                                                           15    9605
XP_006719477                                                           15    9605
XP_006719476                                                           15    9605
XP_006719475                                                           15    9605
XP_005263120                                                           15    9605
XP_006711789                                                           15    9605
XP_005273054                                                           15    9605
XP_006711788                                                           15    9605
XP_005273052                                                           15    9605
XP_005273051                                                           15    9605
>NP_001239058
          Length = 125

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1  MIKEVLVAK 9
          M+KE +VAK
Sbjct: 64 MVKEEVVAK 72
>NP_036453
          Length = 139

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1  MIKEVLVAK 9
          M+KE +VAK
Sbjct: 78 MVKEEVVAK 86
>NP_001014431
          Length = 480

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   KEVLVAK 9
           KEV+VAK
Sbjct: 183 KEVIVAK 189
>NP_005154
          Length = 480

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   KEVLVAK 9
           KEV+VAK
Sbjct: 183 KEVIVAK 189
>NP_001014432
          Length = 480

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   KEVLVAK 9
           KEV+VAK
Sbjct: 183 KEVIVAK 189
>NP_004906
          Length = 678

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   MIKEVLVA 8
           M+KE+L A
Sbjct: 196 MVKEILTA 203
>NP_997511
          Length = 644

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   MIKEVLVA 8
           M+KE+L A
Sbjct: 174 MVKEILTA 181
>NP_058198
          Length = 666

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   MIKEVLVA 8
           M+KE+L A
Sbjct: 196 MVKEILTA 203
>NP_997512
          Length = 663

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   MIKEVLVA 8
           M+KE+L A
Sbjct: 193 MVKEILTA 200
>NP_997510
          Length = 668

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   MIKEVLVA 8
           M+KE+L A
Sbjct: 198 MVKEILTA 205
>NP_997057
          Length = 677

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   MIKEVLVA 8
           M+KE+L A
Sbjct: 207 MVKEILTA 214
>XP_005267458
          Length = 480

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   KEVLVAK 9
           KEV+VAK
Sbjct: 183 KEVIVAK 189
>XP_005262788
          Length = 295

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   MIKEVLVAK 9
           +IKE+ VAK
Sbjct: 152 VIKEISVAK 160
>NP_001095940
          Length = 375

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   MIKEVLVAK 9
           +IKE+ VAK
Sbjct: 152 VIKEISVAK 160
>NP_000663
          Length = 368

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   MIKEVLVAK 9
           +IKE+ VAK
Sbjct: 152 VIKEISVAK 160
>NP_001124438
          Length = 534

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   MIKEVLV 7
           MIK+VLV
Sbjct: 96  MIKQVLV 102
>NP_001159726
          Length = 466

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1  MIKEVLV 7
          MIK+VLV
Sbjct: 28 MIKQVLV 34
>NP_001159725
          Length = 580

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   MIKEVLV 7
           MIK+VLV
Sbjct: 96  MIKQVLV 102
>NP_001052
          Length = 534

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   MIKEVLV 7
           MIK+VLV
Sbjct: 96  MIKQVLV 102
>NP_112246
          Length = 460

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   MIKEVLV 7
           MIK+VLV
Sbjct: 96  MIKQVLV 102
>NP_001193658
          Length = 465

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   KEVLVAK 9
           KEV++AK
Sbjct: 181 KEVIIAK 187
>NP_859029
          Length = 465

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   KEVLVAK 9
           KEV++AK
Sbjct: 181 KEVIIAK 187
>NP_005456
          Length = 479

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   KEVLVAK 9
           KEV++AK
Sbjct: 181 KEVIIAK 187
>NP_001617
          Length = 481

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   KEVLVAK 9
           KEV++AK
Sbjct: 185 KEVIIAK 191
>NP_001229957
          Length = 419

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   KEVLVAK 9
           KEV++AK
Sbjct: 123 KEVIIAK 129
>NP_001229956
          Length = 419

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   KEVLVAK 9
           KEV++AK
Sbjct: 123 KEVIIAK 129
>NP_004555
          Length = 557

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   MIKEVLVAK 9
           MIKE+L  K
Sbjct: 546 MIKEILEKK 554
>XP_006725026
          Length = 247

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   KEVLVAK 9
           KEV++AK
Sbjct: 181 KEVIIAK 187
>XP_006725025
          Length = 418

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   KEVLVAK 9
           KEV++AK
Sbjct: 120 KEVIIAK 126
>XP_006725024
          Length = 465

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   KEVLVAK 9
           KEV++AK
Sbjct: 181 KEVIIAK 187
>XP_006725023
          Length = 479

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   KEVLVAK 9
           KEV++AK
Sbjct: 181 KEVIIAK 187
>XP_006725022
          Length = 479

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   KEVLVAK 9
           KEV++AK
Sbjct: 181 KEVIIAK 187
>XP_006723148
          Length = 537

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   KEVLVAK 9
           KEV++AK
Sbjct: 185 KEVIIAK 191
>XP_006723147
          Length = 537

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   KEVLVAK 9
           KEV++AK
Sbjct: 185 KEVIIAK 191
>XP_006723146
          Length = 537

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   KEVLVAK 9
           KEV++AK
Sbjct: 185 KEVIIAK 191
>XP_006723145
          Length = 537

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   KEVLVAK 9
           KEV++AK
Sbjct: 185 KEVIIAK 191
>XP_006723144
          Length = 537

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   KEVLVAK 9
           KEV++AK
Sbjct: 185 KEVIIAK 191
>XP_006719480
          Length = 2035

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2    IKEVLVA 8
            +KEVLVA
Sbjct: 1634 LKEVLVA 1640
>XP_006719479
          Length = 2059

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2    IKEVLVA 8
            +KEVLVA
Sbjct: 1658 LKEVLVA 1664
>XP_006719478
          Length = 2078

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2    IKEVLVA 8
            +KEVLVA
Sbjct: 1681 LKEVLVA 1687
>XP_006719477
          Length = 2082

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2    IKEVLVA 8
            +KEVLVA
Sbjct: 1681 LKEVLVA 1687
>XP_006719476
          Length = 2082

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2    IKEVLVA 8
            +KEVLVA
Sbjct: 1681 LKEVLVA 1687
>XP_006719475
          Length = 2082

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2    IKEVLVA 8
            +KEVLVA
Sbjct: 1681 LKEVLVA 1687
>XP_005263120
          Length = 512

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   MIKEVLVAK 9
           MIKE+L  K
Sbjct: 501 MIKEILEKK 509
>XP_006711789
          Length = 247

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   KEVLVAK 9
           KEV++AK
Sbjct: 181 KEVIIAK 187
>XP_005273054
          Length = 418

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   KEVLVAK 9
           KEV++AK
Sbjct: 120 KEVIIAK 126
>XP_006711788
          Length = 465

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   KEVLVAK 9
           KEV++AK
Sbjct: 181 KEVIIAK 187
>XP_005273052
          Length = 479

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   KEVLVAK 9
           KEV++AK
Sbjct: 181 KEVIIAK 187
>XP_005273051
          Length = 479

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   KEVLVAK 9
           KEV++AK
Sbjct: 181 KEVIIAK 187
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.325    0.136    0.312 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 325,794
Number of extensions: 354
Number of successful extensions: 49
Number of sequences better than 10000.0: 49
Number of HSP's gapped: 49
Number of HSP's successfully gapped: 49
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.0 bits)
S2: 28 (15.4 bits)

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