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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_065681                                                              18    1482
NP_066124                                                              18    1482
XP_006717999                                                           18    1482
NP_001264214                                                           17    4312
XP_006718858                                                           17    4312
NP_653233                                                              16    7355
NP_001364                                                              15    9605
NP_001258736                                                           15    9605
NP_115915                                                              15    9605
NP_835468                                                              15    9605
NP_001258737                                                           15    9605
XP_006711798                                                           15    9605

>NP_065681
          Length = 1072

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   MIAAAVLFS 9
           +IAAAVLFS
Sbjct: 637 VIAAAVLFS 645



>NP_066124
          Length = 1114

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   MIAAAVLFS 9
           +IAAAVLFS
Sbjct: 637 VIAAAVLFS 645



>XP_006717999
          Length = 1207

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   MIAAAVLFS 9
           +IAAAVLFS
Sbjct: 730 VIAAAVLFS 738



>NP_001264214
          Length = 1437

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2   IAAAVLFS 9
           +AAA+LFS
Sbjct: 734 LAAAILFS 741



>XP_006718858
          Length = 913

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2   IAAAVLFS 9
           +AAA+LFS
Sbjct: 210 LAAAILFS 217



>NP_653233
          Length = 229

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   IAAAVLFS 9
           IAA +LFS
Sbjct: 154 IAAGILFS 161



>NP_001364
          Length = 4515

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1    MIAAAVLFS 9
            MIA  +LFS
Sbjct: 1680 MIAEIILFS 1688



>NP_001258736
          Length = 199

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   MIAAAVLFS 9
           ++AA VLF+
Sbjct: 174 LVAAVVLFA 182



>NP_115915
          Length = 368

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   IAAAVLFS 9
           IAAA +FS
Sbjct: 187 IAAATIFS 194



>NP_835468
          Length = 311

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   MIAAAVLFS 9
           ++AA VLF+
Sbjct: 174 LVAAVVLFA 182



>NP_001258737
          Length = 200

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1  MIAAAVLFS 9
          ++AA VLF+
Sbjct: 63 LVAAVVLFA 71



>XP_006711798
          Length = 4586

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1    MIAAAVLFS 9
            MIA  +LFS
Sbjct: 1697 MIAEIILFS 1705



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.346    0.137    0.332 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 271,767
Number of extensions: 293
Number of successful extensions: 12
Number of sequences better than 10000.0: 12
Number of HSP's gapped: 12
Number of HSP's successfully gapped: 12
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.5 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.8 bits)
S2: 28 (15.4 bits)