Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_006715                                                              18    1935
NP_005913                                                              18    1935
XP_005267046                                                           18    1935
XP_005267045                                                           18    1935
NP_001286                                                              16    5631
NP_006741                                                              16    7355
NP_005017                                                              15    9605
NP_001245340                                                           15    9605
NP_001245342                                                           15    9605
NP_443176                                                              15    9605
NP_001245341                                                           15    9605
NP_001273988                                                           15    9605
NP_055867                                                              15    9605
NP_055429                                                              15    9605
NP_001245343                                                           15    9605
XP_005261547                                                           15    9605
XP_005255139                                                           15    9605
XP_006721078                                                           15    9605
XP_005255138                                                           15    9605
XP_006721077                                                           15    9605
XP_005255137                                                           15    9605
XP_006715736                                                           15    9605
XP_006715735                                                           15    9605
XP_005249722                                                           15    9605
XP_006715734                                                           15    9605
XP_006715733                                                           15    9605
XP_006712032                                                           15    9605
XP_006710753                                                           15    9605
XP_005263530                                                           15    9605
XP_006710752                                                           15    9605
XP_006710751                                                           15    9605
XP_006710750                                                           15    9605
XP_006710749                                                           15    9605
NP_056185                                                              15    9605
>NP_006715
          Length = 1558

 Score = 17.7 bits (34), Expect =  1935
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   MGLIKLILR 9
           MGL KLILR
Sbjct: 537 MGLRKLILR 545
>NP_005913
          Length = 1608

 Score = 17.7 bits (34), Expect =  1935
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   MGLIKLILR 9
           MGL KLILR
Sbjct: 537 MGLRKLILR 545
>XP_005267046
          Length = 1554

 Score = 17.7 bits (34), Expect =  1935
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   MGLIKLILR 9
           MGL KLILR
Sbjct: 537 MGLRKLILR 545
>XP_005267045
          Length = 1604

 Score = 17.7 bits (34), Expect =  1935
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   MGLIKLILR 9
           MGL KLILR
Sbjct: 537 MGLRKLILR 545
>NP_001286
          Length = 355

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   GLIKLILR 9
           G+IK++LR
Sbjct: 221 GIIKILLR 228
>NP_006741
          Length = 540

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2  GLIKLILR 9
          GL++L+LR
Sbjct: 26 GLVELLLR 33
>NP_005017
          Length = 1044

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   GLIKLILR 9
           GLI+++LR
Sbjct: 823 GLIEVVLR 830
>NP_001245340
          Length = 640

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 1   MGLIKLIL 8
           +GL++L+L
Sbjct: 455 LGLVRLVL 462
>NP_001245342
          Length = 611

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 1   MGLIKLIL 8
           +GL++L+L
Sbjct: 426 LGLVRLVL 433
>NP_443176
          Length = 675

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 1   MGLIKLIL 8
           +GL++L+L
Sbjct: 490 LGLVRLVL 497
>NP_001245341
          Length = 605

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 1   MGLIKLIL 8
           +GL++L+L
Sbjct: 420 LGLVRLVL 427
>NP_001273988
          Length = 1572

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2    GLIKLILR 9
            G IKLI+R
Sbjct: 1214 GKIKLIIR 1221
>NP_055867
          Length = 1606

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2    GLIKLILR 9
            G IKLI+R
Sbjct: 1248 GKIKLIIR 1255
>NP_055429
          Length = 1843

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1    MGLIKLILR 9
            +GL+ L+LR
Sbjct: 1168 IGLLSLLLR 1176
>NP_001245343
          Length = 519

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 1   MGLIKLIL 8
           +GL++L+L
Sbjct: 334 LGLVRLVL 341
>XP_005261547
          Length = 249

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3  LIKLILR 9
          L+KL+LR
Sbjct: 35 LVKLVLR 41
>XP_005255139
          Length = 514

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 1   MGLIKLIL 8
           +GL++L+L
Sbjct: 329 LGLVRLVL 336
>XP_006721078
          Length = 576

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 1   MGLIKLIL 8
           +GL++L+L
Sbjct: 391 LGLVRLVL 398
>XP_005255138
          Length = 583

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 1   MGLIKLIL 8
           +GL++L+L
Sbjct: 398 LGLVRLVL 405
>XP_006721077
          Length = 655

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 1   MGLIKLIL 8
           +GL++L+L
Sbjct: 470 LGLVRLVL 477
>XP_005255137
          Length = 675

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 1   MGLIKLIL 8
           +GL++L+L
Sbjct: 490 LGLVRLVL 497
>XP_006715736
          Length = 1573

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2    GLIKLILR 9
            G IKLI+R
Sbjct: 1215 GKIKLIIR 1222
>XP_006715735
          Length = 1606

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2    GLIKLILR 9
            G IKLI+R
Sbjct: 1248 GKIKLIIR 1255
>XP_005249722
          Length = 1607

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2    GLIKLILR 9
            G IKLI+R
Sbjct: 1249 GKIKLIIR 1256
>XP_006715734
          Length = 1607

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2    GLIKLILR 9
            G IKLI+R
Sbjct: 1249 GKIKLIIR 1256
>XP_006715733
          Length = 1608

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2    GLIKLILR 9
            G IKLI+R
Sbjct: 1250 GKIKLIIR 1257
>XP_006712032
          Length = 1001

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   MGLIKLILR 9
           +GL+ L+LR
Sbjct: 326 IGLLSLLLR 334
>XP_006710753
          Length = 1015

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   GLIKLILR 9
           GLI+++LR
Sbjct: 794 GLIEVVLR 801
>XP_005263530
          Length = 1043

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   GLIKLILR 9
           GLI+++LR
Sbjct: 822 GLIEVVLR 829
>XP_006710752
          Length = 1044

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   GLIKLILR 9
           GLI+++LR
Sbjct: 823 GLIEVVLR 830
>XP_006710751
          Length = 1044

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   GLIKLILR 9
           GLI+++LR
Sbjct: 823 GLIEVVLR 830
>XP_006710750
          Length = 1044

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   GLIKLILR 9
           GLI+++LR
Sbjct: 823 GLIEVVLR 830
>XP_006710749
          Length = 1044

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   GLIKLILR 9
           GLI+++LR
Sbjct: 823 GLIEVVLR 830
>NP_056185
          Length = 290

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3  LIKLILR 9
          L+KL+LR
Sbjct: 76 LVKLVLR 82
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.347    0.169    0.442 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 420,286
Number of extensions: 810
Number of successful extensions: 34
Number of sequences better than 10000.0: 34
Number of HSP's gapped: 34
Number of HSP's successfully gapped: 34
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 14 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.6 bits)
S2: 28 (15.4 bits)

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