Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_115927                                                              20     509
XP_006715292                                                           20     509
NP_002418                                                              17    3301
NP_002413                                                              17    3301
NP_002416                                                              17    3301
NP_055791                                                              17    4312
NP_003038                                                              17    4312
XP_006720419                                                           17    4312
XP_005269704                                                           17    4312
NP_071405                                                              17    4312
NP_008973                                                              16    5631
NP_689669                                                              16    5631
NP_001166242                                                           16    5631
NP_001166243                                                           16    5631
NP_001092253                                                           16    5631
NP_000544                                                              16    7355
NP_001244336                                                           15    9605
NP_000409                                                              15    9605
NP_001244335                                                           15    9605
NP_001157745                                                           15    9605
NP_001074010                                                           15    9605
NP_001093869                                                           15    9605
NP_631902                                                              15    9605
NP_001276922                                                           15    9605
NP_001120863                                                           15    9605
XP_005255366                                                           15    9605
XP_005255362                                                           15    9605
XP_005254311                                                           15    9605
XP_006720508                                                           15    9605
XP_006720507                                                           15    9605
XP_005254310                                                           15    9605
XP_005254309                                                           15    9605
XP_005254306                                                           15    9605
XP_005254305                                                           15    9605
XP_005254304                                                           15    9605
XP_005254303                                                           15    9605
XP_006720506                                                           15    9605
XP_005254302                                                           15    9605
XP_005254301                                                           15    9605
XP_006720505                                                           15    9605
XP_005254300                                                           15    9605
XP_005262709                                                           15    9605
XP_006712336                                                           15    9605
NP_703148                                                              15    9605
>NP_115927
          Length = 1321

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   MFRVVNVTP 9
           MF VVNVTP
Sbjct: 337 MFGVVNVTP 345
>XP_006715292
          Length = 759

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   MFRVVNVTP 9
           MF VVNVTP
Sbjct: 324 MFGVVNVTP 332
>NP_002418
          Length = 471

 Score = 16.9 bits (32), Expect =  3301
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   FRVVNVTP 9
           +R+VN TP
Sbjct: 120 YRIVNYTP 127
>NP_002413
          Length = 477

 Score = 16.9 bits (32), Expect =  3301
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   FRVVNVTP 9
           +R+VN TP
Sbjct: 116 YRIVNYTP 123
>NP_002416
          Length = 476

 Score = 16.9 bits (32), Expect =  3301
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   FRVVNVTP 9
           +R+VN TP
Sbjct: 115 YRIVNYTP 122
>NP_055791
          Length = 1871

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1    MFRVVNVTP 9
            M RVV VTP
Sbjct: 1234 MGRVVKVTP 1242
>NP_003038
          Length = 815

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   FRVVNVTP 9
           FR+V +TP
Sbjct: 439 FRIVKLTP 446
>XP_006720419
          Length = 138

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   MFRVVNVTP 9
           M +VVN++P
Sbjct: 96  MLQVVNISP 104
>XP_005269704
          Length = 1872

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1    MFRVVNVTP 9
            M RVV VTP
Sbjct: 1234 MGRVVKVTP 1242
>NP_071405
          Length = 513

 Score = 16.5 bits (31), Expect =  4312
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 2   FRVVNVTP 9
           +R++N TP
Sbjct: 113 YRIINYTP 120
>NP_008973
          Length = 124

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   FRVVNVTP 9
           FRV+ V+P
Sbjct: 102 FRVIQVSP 109
>NP_689669
          Length = 242

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1  MFRVVNVT 8
          +FR+ NVT
Sbjct: 71 VFRIANVT 78
>NP_001166242
          Length = 241

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1  MFRVVNVT 8
          +FR+ NVT
Sbjct: 71 VFRIANVT 78
>NP_001166243
          Length = 242

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1  MFRVVNVT 8
          +FR+ NVT
Sbjct: 71 VFRIANVT 78
>NP_001092253
          Length = 124

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   FRVVNVTP 9
           FRV+ V+P
Sbjct: 102 FRVIQVSP 109
>NP_000544
          Length = 1432

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   FRVVNVTP 9
           +R+V VTP
Sbjct: 636 YRIVYVTP 643
>NP_001244336
          Length = 810

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   FRVVNVT 8
           FR+ NVT
Sbjct: 157 FRIYNVT 163
>NP_000409
          Length = 825

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   FRVVNVT 8
           FR+ NVT
Sbjct: 172 FRIYNVT 178
>NP_001244335
          Length = 825

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   FRVVNVT 8
           FR+ NVT
Sbjct: 172 FRIYNVT 178
>NP_001157745
          Length = 3065

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    MFRVVNVT 8
            M R+VNVT
Sbjct: 2648 MPRIVNVT 2655
>NP_001074010
          Length = 2856

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    MFRVVNVT 8
            M R+VNVT
Sbjct: 2439 MPRIVNVT 2446
>NP_001093869
          Length = 1337

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 3    RVVNVTP 9
            R+++VTP
Sbjct: 1203 RIISVTP 1209
>NP_631902
          Length = 445

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 2  FRVVNVTP 9
          + V+NV+P
Sbjct: 78 YEVINVSP 85
>NP_001276922
          Length = 422

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 2  FRVVNVTP 9
          + V+NV+P
Sbjct: 78 YEVINVSP 85
>NP_001120863
          Length = 422

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 2  FRVVNVTP 9
          + V+NV+P
Sbjct: 78 YEVINVSP 85
>XP_005255366
          Length = 364

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   FRVVNVT 8
           FR+ NVT
Sbjct: 232 FRIYNVT 238
>XP_005255362
          Length = 885

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   FRVVNVT 8
           FR+ NVT
Sbjct: 232 FRIYNVT 238
>XP_005254311
          Length = 1627

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    MFRVVNVT 8
            M R+VNVT
Sbjct: 1210 MPRIVNVT 1217
>XP_006720508
          Length = 2658

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    MFRVVNVT 8
            M R+VNVT
Sbjct: 2241 MPRIVNVT 2248
>XP_006720507
          Length = 2763

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    MFRVVNVT 8
            M R+VNVT
Sbjct: 2346 MPRIVNVT 2353
>XP_005254310
          Length = 2906

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    MFRVVNVT 8
            M R+VNVT
Sbjct: 2489 MPRIVNVT 2496
>XP_005254309
          Length = 2956

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    MFRVVNVT 8
            M R+VNVT
Sbjct: 2539 MPRIVNVT 2546
>XP_005254306
          Length = 3066

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    MFRVVNVT 8
            M R+VNVT
Sbjct: 2649 MPRIVNVT 2656
>XP_005254305
          Length = 3114

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    MFRVVNVT 8
            M R+VNVT
Sbjct: 2697 MPRIVNVT 2704
>XP_005254304
          Length = 3115

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    MFRVVNVT 8
            M R+VNVT
Sbjct: 2698 MPRIVNVT 2705
>XP_005254303
          Length = 3115

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    MFRVVNVT 8
            M R+VNVT
Sbjct: 2698 MPRIVNVT 2705
>XP_006720506
          Length = 3115

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    MFRVVNVT 8
            M R+VNVT
Sbjct: 2698 MPRIVNVT 2705
>XP_005254302
          Length = 3115

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    MFRVVNVT 8
            M R+VNVT
Sbjct: 2698 MPRIVNVT 2705
>XP_005254301
          Length = 3115

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    MFRVVNVT 8
            M R+VNVT
Sbjct: 2698 MPRIVNVT 2705
>XP_006720505
          Length = 3115

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    MFRVVNVT 8
            M R+VNVT
Sbjct: 2698 MPRIVNVT 2705
>XP_005254300
          Length = 3115

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    MFRVVNVT 8
            M R+VNVT
Sbjct: 2698 MPRIVNVT 2705
>XP_005262709
          Length = 1337

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 3    RVVNVTP 9
            R+++VTP
Sbjct: 1203 RIISVTP 1209
>XP_006712336
          Length = 262

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 2  FRVVNVTP 9
          + V+NV+P
Sbjct: 78 YEVINVSP 85
>NP_703148
          Length = 1200

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 6/9 (66%)

Query: 1   MFRVVNVTP 9
           MFR   +TP
Sbjct: 816 MFRNAQITP 824
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.338    0.143    0.433 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 366,657
Number of extensions: 633
Number of successful extensions: 44
Number of sequences better than 10000.0: 44
Number of HSP's gapped: 44
Number of HSP's successfully gapped: 44
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.4 bits)
S2: 28 (15.4 bits)

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