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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_004110                                                              20     509
NP_078932                                                              18    1935
XP_005260137                                                           18    1935
NP_001074419                                                           17    4312
NP_001074248                                                           17    4312
NP_203693                                                              17    4312
XP_005256712                                                           17    4312
NP_000325                                                              15    9605
XP_005257623                                                           15    9605

>NP_004110
          Length = 993

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   MFMLNIRRK 9
           MF LNIRRK
Sbjct: 430 MFTLNIRRK 438



>NP_078932
          Length = 622

 Score = 17.7 bits (34), Expect =  1935
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1   MFMLNIRRK 9
           +FM+N+RR+
Sbjct: 343 VFMINLRRR 351



>XP_005260137
          Length = 539

 Score = 17.7 bits (34), Expect =  1935
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1   MFMLNIRRK 9
           +FM+N+RR+
Sbjct: 260 VFMINLRRR 268



>NP_001074419
          Length = 1044

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   FMLNIRRK 9
           F+LN+RR+
Sbjct: 797 FLLNLRRQ 804



>NP_001074248
          Length = 1063

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   FMLNIRRK 9
           F+LN+RR+
Sbjct: 816 FLLNLRRQ 823



>NP_203693
          Length = 1028

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   FMLNIRRK 9
           F+LN+RR+
Sbjct: 781 FLLNLRRQ 788



>XP_005256712
          Length = 1039

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   FMLNIRRK 9
           F+LN+RR+
Sbjct: 792 FLLNLRRQ 799



>NP_000325
          Length = 1836

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   MFMLNIRRK 9
           +FM N+R+K
Sbjct: 271 LFMGNLRQK 279



>XP_005257623
          Length = 1836

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   MFMLNIRRK 9
           +FM N+R+K
Sbjct: 271 LFMGNLRQK 279



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.357    0.154    0.436 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 361,716
Number of extensions: 383
Number of successful extensions: 9
Number of sequences better than 10000.0: 9
Number of HSP's gapped: 9
Number of HSP's successfully gapped: 9
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 14 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (17.1 bits)
S2: 28 (15.4 bits)