Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_055365                                                              20     390
NP_663780                                                              18    1482
NP_056101                                                              18    1482
NP_001139784                                                           17    4312
NP_705832                                                              17    4312
NP_055160                                                              17    4312
XP_005256921                                                           17    4312
XP_005256920                                                           17    4312
NP_008880                                                              16    5631
NP_001258963                                                           16    5631
NP_001120868                                                           16    5631
NP_004371                                                              16    5631
NP_001073315                                                           16    5631
NP_001004334                                                           16    5631
XP_003846519                                                           16    5631
XP_006722914                                                           16    5631
XP_005255182                                                           16    5631
XP_006720911                                                           16    5631
XP_005255181                                                           16    5631
NP_001968                                                              16    5631
NP_004908                                                              16    7355
NP_001243779                                                           16    7355
NP_596869                                                              16    7355
NP_055625                                                              16    7355
NP_775885                                                              16    7355
NP_001004060                                                           16    7355
NP_001004067                                                           16    7355
NP_071941                                                              16    7355
XP_006725258                                                           16    7355
XP_006721059                                                           16    7355
XP_005255375                                                           16    7355
XP_006712787                                                           16    7355
XP_006712786                                                           16    7355
XP_006711734                                                           16    7355
XP_006711733                                                           16    7355
XP_006711732                                                           16    7355
XP_006711731                                                           16    7355
XP_006711730                                                           16    7355
XP_006711729                                                           16    7355
XP_005245695                                                           16    7355
XP_006710314                                                           16    7355
NP_055102                                                              16    7355
NP_001254479                                                           16    7355
NP_001073991                                                           15    9605
NP_065836                                                              15    9605
NP_001158192                                                           15    9605
NP_006011                                                              15    9605
NP_005413                                                              15    9605
XP_006721765                                                           15    9605
XP_006721764                                                           15    9605
XP_005272505                                                           15    9605
XP_006720838                                                           15    9605
XP_005271710                                                           15    9605
XP_006714033                                                           15    9605
XP_005248236                                                           15    9605
XP_005248234                                                           15    9605
NP_149124                                                              15    9605
>NP_055365
          Length = 1032

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   MEPREQMVE 9
           MEPREQ VE
Sbjct: 645 MEPREQRVE 653
>NP_663780
          Length = 1565

 Score = 18.1 bits (35), Expect =  1482
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2   EPREQMVE 9
           EPR+Q+VE
Sbjct: 944 EPRQQLVE 951
>NP_056101
          Length = 1253

 Score = 18.1 bits (35), Expect =  1482
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2   EPREQMVE 9
           EPR+Q+VE
Sbjct: 944 EPRQQLVE 951
>NP_001139784
          Length = 986

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2   EPREQMV 8
           EP EQMV
Sbjct: 325 EPNEQMV 331
>NP_705832
          Length = 938

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2   EPREQMV 8
           EP EQMV
Sbjct: 325 EPNEQMV 331
>NP_055160
          Length = 2279

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3    PREQMVE 9
            PREQ++E
Sbjct: 2245 PREQVIE 2251
>XP_005256921
          Length = 907

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2   EPREQMV 8
           EP EQMV
Sbjct: 246 EPNEQMV 252
>XP_005256920
          Length = 943

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2   EPREQMV 8
           EP EQMV
Sbjct: 325 EPNEQMV 331
>NP_008880
          Length = 593

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   MEPREQM 7
           +EPRE+M
Sbjct: 460 LEPRERM 466
>NP_001258963
          Length = 604

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   MEPREQM 7
           +EPRE+M
Sbjct: 471 LEPRERM 477
>NP_001120868
          Length = 590

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   MEPREQM 7
           +EPRE+M
Sbjct: 457 LEPRERM 463
>NP_004371
          Length = 2442

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2    EPREQMVE 9
            EPR +M+E
Sbjct: 1016 EPRSEMME 1023
>NP_001073315
          Length = 2404

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   EPREQMVE 9
           EPR +M+E
Sbjct: 978 EPRSEMME 985
>NP_001004334
          Length = 2367

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   PREQMVE 9
           PRE+MV+
Sbjct: 638 PREEMVD 644
>XP_003846519
          Length = 161

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   MEPREQMVE 9
           MEP E+M E
Sbjct: 140 MEPHEKMGE 148
>XP_006722914
          Length = 566

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   MEPREQM 7
           +EPRE+M
Sbjct: 433 LEPRERM 439
>XP_005255182
          Length = 2303

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   EPREQMVE 9
           EPR +M+E
Sbjct: 877 EPRSEMME 884
>XP_006720911
          Length = 2355

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2    EPREQMVE 9
            EPR +M+E
Sbjct: 1016 EPRSEMME 1023
>XP_005255181
          Length = 2427

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2    EPREQMVE 9
            EPR +M+E
Sbjct: 1001 EPRSEMME 1008
>NP_001968
          Length = 957

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   EPREQMVE 9
           +PREQ++E
Sbjct: 923 KPREQVLE 930
>NP_004908
          Length = 254

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2  EPREQMVE 9
          EP  QMVE
Sbjct: 91 EPGSQMVE 98
>NP_001243779
          Length = 34350

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1    MEPREQMVE 9
            M PRE++V+
Sbjct: 9930 MTPREEIVK 9938
>NP_596869
          Length = 33423

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1    MEPREQMVE 9
            M PRE++V+
Sbjct: 9003 MTPREEIVK 9011
>NP_055625
          Length = 3117

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   MEPREQ 6
           MEP+EQ
Sbjct: 443 MEPKEQ 448
>NP_775885
          Length = 1222

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2   EPREQMVE 9
           EP  QM+E
Sbjct: 936 EPSSQMIE 943
>NP_001004060
          Length = 1267

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2   EPREQMVE 9
           EP  QM+E
Sbjct: 936 EPSSQMIE 943
>NP_001004067
          Length = 1222

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2   EPREQMVE 9
           EP  QM+E
Sbjct: 936 EPSSQMIE 943
>NP_071941
          Length = 396

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   EPREQMV 8
           +PRE+MV
Sbjct: 250 KPREEMV 256
>XP_006725258
          Length = 1267

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2   EPREQMVE 9
           EP  QM+E
Sbjct: 936 EPSSQMIE 943
>XP_006721059
          Length = 294

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2  EPREQMVE 9
          EP  QM+E
Sbjct: 8  EPSSQMIE 15
>XP_005255375
          Length = 1267

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2   EPREQMVE 9
           EP  QM+E
Sbjct: 936 EPSSQMIE 943
>XP_006712787
          Length = 33978

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1    MEPREQMVE 9
            M PRE++V+
Sbjct: 9931 MTPREEIVK 9939
>XP_006712786
          Length = 35484

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1    MEPREQMVE 9
            M PRE++V+
Sbjct: 9931 MTPREEIVK 9939
>XP_006711734
          Length = 3136

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   MEPREQ 6
           MEP+EQ
Sbjct: 421 MEPKEQ 426
>XP_006711733
          Length = 3137

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   MEPREQ 6
           MEP+EQ
Sbjct: 422 MEPKEQ 427
>XP_006711732
          Length = 3154

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   MEPREQ 6
           MEP+EQ
Sbjct: 443 MEPKEQ 448
>XP_006711731
          Length = 3157

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   MEPREQ 6
           MEP+EQ
Sbjct: 443 MEPKEQ 448
>XP_006711730
          Length = 3157

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   MEPREQ 6
           MEP+EQ
Sbjct: 442 MEPKEQ 447
>XP_006711729
          Length = 3158

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   MEPREQ 6
           MEP+EQ
Sbjct: 443 MEPKEQ 448
>XP_005245695
          Length = 3158

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   MEPREQ 6
           MEP+EQ
Sbjct: 443 MEPKEQ 448
>XP_006710314
          Length = 193

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2  EPREQMVE 9
          EP  QM+E
Sbjct: 8  EPSSQMIE 15
>NP_055102
          Length = 1222

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2   EPREQMVE 9
           EP  QM+E
Sbjct: 936 EPSSQMIE 943
>NP_001254479
          Length = 35991

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1     MEPREQMVE 9
             M PRE++V+
Sbjct: 10247 MTPREEIVK 10255
>NP_001073991
          Length = 1620

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1 MEPREQMVE 9
         M PRE+ V+
Sbjct: 1 MNPREEKVK 9
>NP_065836
          Length = 122

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1 MEPREQMVE 9
         M PRE+ V+
Sbjct: 1 MNPREEKVK 9
>NP_001158192
          Length = 111

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1 MEPREQMVE 9
         M PRE+ V+
Sbjct: 1 MNPREEKVK 9
>NP_006011
          Length = 389

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   PREQMVE 9
           PR+ MVE
Sbjct: 314 PRDSMVE 320
>NP_005413
          Length = 2155

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 2    EPREQMV 8
            EP+EQ++
Sbjct: 2069 EPKEQII 2075
>XP_006721765
          Length = 401

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   EPREQM 7
           EPRE+M
Sbjct: 211 EPREKM 216
>XP_006721764
          Length = 401

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   EPREQM 7
           EPRE+M
Sbjct: 211 EPREKM 216
>XP_005272505
          Length = 353

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2  EPREQMV 8
          EPREQ +
Sbjct: 10 EPREQPI 16
>XP_006720838
          Length = 364

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2  EPREQMV 8
          EPREQ +
Sbjct: 10 EPREQPI 16
>XP_005271710
          Length = 2150

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 2    EPREQMV 8
            EP+EQ++
Sbjct: 2064 EPKEQII 2070
>XP_006714033
          Length = 111

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1 MEPREQMVE 9
         M PRE+ V+
Sbjct: 1 MNPREEKVK 9
>XP_005248236
          Length = 1262

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1 MEPREQMVE 9
         M PRE+ V+
Sbjct: 1 MNPREEKVK 9
>XP_005248234
          Length = 1620

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1 MEPREQMVE 9
         M PRE+ V+
Sbjct: 1 MNPREEKVK 9
>NP_149124
          Length = 421

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   EPREQM 7
           EPRE+M
Sbjct: 231 EPREKM 236
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.309    0.123    0.319 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 795,658
Number of extensions: 1090
Number of successful extensions: 57
Number of sequences better than 10000.0: 57
Number of HSP's gapped: 57
Number of HSP's successfully gapped: 57
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.5 bits)
S2: 28 (15.4 bits)

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