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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_001275701                                                           21     229
NP_004561                                                              21     229
NP_001275703                                                           21     229
XP_005253450                                                           21     229
XP_006719151                                                           21     229
NP_005872                                                              17    4312
NP_001258855                                                           17    4312
NP_001116429                                                           17    4312
NP_001138890                                                           16    5631
NP_001041                                                              16    5631
XP_006724234                                                           16    5631
XP_005261462                                                           16    5631
NP_001171122                                                           16    7355
NP_006350                                                              16    7355
NP_001036002                                                           16    7355
NP_848620                                                              16    7355
XP_006724789                                                           16    7355

>NP_001275701
          Length = 1486

 Score = 20.8 bits (42), Expect =   229
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    MEMMLYAGL 9
            +EMMLYAGL
Sbjct: 1158 LEMMLYAGL 1166



>NP_004561
          Length = 1445

 Score = 20.8 bits (42), Expect =   229
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    MEMMLYAGL 9
            +EMMLYAGL
Sbjct: 1117 LEMMLYAGL 1125



>NP_001275703
          Length = 1264

 Score = 20.8 bits (42), Expect =   229
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   MEMMLYAGL 9
           +EMMLYAGL
Sbjct: 936 LEMMLYAGL 944



>XP_005253450
          Length = 1486

 Score = 20.8 bits (42), Expect =   229
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    MEMMLYAGL 9
            +EMMLYAGL
Sbjct: 1158 LEMMLYAGL 1166



>XP_006719151
          Length = 1486

 Score = 20.8 bits (42), Expect =   229
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    MEMMLYAGL 9
            +EMMLYAGL
Sbjct: 1158 LEMMLYAGL 1166



>NP_005872
          Length = 412

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3  MMLYAG 8
          MMLYAG
Sbjct: 74 MMLYAG 79



>NP_001258855
          Length = 335

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3  MMLYAG 8
          MMLYAG
Sbjct: 74 MMLYAG 79



>NP_001116429
          Length = 365

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3  MMLYAG 8
          MMLYAG
Sbjct: 74 MMLYAG 79



>NP_001138890
          Length = 2481

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   MEMMLYAGL 9
           +EM  YAGL
Sbjct: 432 IEMRAYAGL 440



>NP_001041
          Length = 369

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   MEMMLYAGL 9
           + +M+YAGL
Sbjct: 175 LPIMIYAGL 183



>XP_006724234
          Length = 2363

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   MEMMLYAGL 9
           +EM  YAGL
Sbjct: 314 IEMRAYAGL 322



>XP_005261462
          Length = 2473

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   MEMMLYAGL 9
           +EM  YAGL
Sbjct: 432 IEMRAYAGL 440



>NP_001171122
          Length = 649

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   MMLYAGL 9
           MM++AGL
Sbjct: 431 MMMFAGL 437



>NP_006350
          Length = 669

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   MMLYAGL 9
           MM++AGL
Sbjct: 451 MMMFAGL 457



>NP_001036002
          Length = 701

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   MMLYAGL 9
           MM++AGL
Sbjct: 483 MMMFAGL 489



>NP_848620
          Length = 416

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   EMMLYAGL 9
           EM+L AGL
Sbjct: 234 EMLLQAGL 241



>XP_006724789
          Length = 649

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   MMLYAGL 9
           MM++AGL
Sbjct: 431 MMMFAGL 437



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.336    0.142    0.394 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 368,533
Number of extensions: 326
Number of successful extensions: 17
Number of sequences better than 10000.0: 17
Number of HSP's gapped: 17
Number of HSP's successfully gapped: 17
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.4 bits)
S2: 28 (15.4 bits)