Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_004110                                                              20     390
NP_689939                                                              18    1935
XP_005274116                                                           17    2528
XP_006718652                                                           17    2528
XP_005274115                                                           17    2528
XP_005274114                                                           17    2528
NP_000244                                                              17    4312
XP_005263082                                                           17    4312
NP_620706                                                              16    5631
NP_003066                                                              16    5631
NP_001123892                                                           16    5631
NP_001189398                                                           16    5631
XP_005269161                                                           16    5631
XP_005269160                                                           16    5631
XP_005269159                                                           16    5631
XP_005269158                                                           16    5631
NP_000107                                                              16    7355
NP_851828                                                              16    7355
NP_620155                                                              16    7355
NP_543006                                                              16    7355
NP_071353                                                              16    7355
NP_064581                                                              16    7355
NP_061026                                                              16    7355
NP_001157936                                                           16    7355
XP_006724905                                                           16    7355
XP_006724904                                                           16    7355
XP_006724899                                                           16    7355
XP_005260806                                                           16    7355
XP_005257627                                                           16    7355
XP_006712770                                                           16    7355
XP_006712325                                                           16    7355
NP_001034597                                                           16    7355
NP_001474                                                              15    9605
NP_001122074                                                           15    9605
NP_005730                                                              15    9605
NP_004068                                                              15    9605
NP_066990                                                              15    9605
NP_060124                                                              15    9605
NP_002067                                                              15    9605
NP_071446                                                              15    9605
NP_060557                                                              15    9605
NP_001129316                                                           15    9605
NP_001180236                                                           15    9605
XP_006723598                                                           15    9605
XP_005254171                                                           15    9605
XP_005245370                                                           15    9605
XP_006710825                                                           15    9605
NP_001162023                                                           15    9605
>NP_004110
          Length = 993

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1   MEFLEFKS 8
           MEFLEFKS
Sbjct: 799 MEFLEFKS 806
>NP_689939
          Length = 434

 Score = 17.7 bits (34), Expect =  1935
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   MEFLEFKSG 9
           MEFL+F  G
Sbjct: 266 MEFLQFSKG 274
>XP_005274116
          Length = 1602

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   EFLEFKSG 9
           EFLEF +G
Sbjct: 896 EFLEFSAG 903
>XP_006718652
          Length = 2196

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2    EFLEFKSG 9
            EFLEF +G
Sbjct: 1466 EFLEFSAG 1473
>XP_005274115
          Length = 2222

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2    EFLEFKSG 9
            EFLEF +G
Sbjct: 1492 EFLEFSAG 1499
>XP_005274114
          Length = 2223

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2    EFLEFKSG 9
            EFLEF +G
Sbjct: 1493 EFLEFSAG 1500
>NP_000244
          Length = 894

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   MEFLEFKS 8
           ++FL+FKS
Sbjct: 376 LDFLDFKS 383
>XP_005263082
          Length = 921

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   MEFLEFKS 8
           ++FL+FKS
Sbjct: 403 LDFLDFKS 410
>NP_620706
          Length = 1130

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3  FLEFKSG 9
          FL+FK+G
Sbjct: 81 FLDFKAG 87
>NP_003066
          Length = 1214

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3  FLEFKSG 9
          FL+FK+G
Sbjct: 81 FLDFKAG 87
>NP_001123892
          Length = 1152

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3  FLEFKSG 9
          FL+FK+G
Sbjct: 81 FLDFKAG 87
>NP_001189398
          Length = 247

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   MEFLEFK 7
           +EFLEF+
Sbjct: 109 LEFLEFR 115
>XP_005269161
          Length = 1151

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3  FLEFKSG 9
          FL+FK+G
Sbjct: 81 FLDFKAG 87
>XP_005269160
          Length = 1213

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3  FLEFKSG 9
          FL+FK+G
Sbjct: 81 FLDFKAG 87
>XP_005269159
          Length = 1244

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3  FLEFKSG 9
          FL+FK+G
Sbjct: 81 FLDFKAG 87
>XP_005269158
          Length = 1245

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3  FLEFKSG 9
          FL+FK+G
Sbjct: 81 FLDFKAG 87
>NP_000107
          Length = 292

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2   EFLEFKSG 9
           EFL FK G
Sbjct: 188 EFLRFKWG 195
>NP_851828
          Length = 262

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2   EFLEFKSG 9
           EFL FK G
Sbjct: 158 EFLRFKWG 165
>NP_620155
          Length = 446

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   FLEFKSG 9
           FL+F+SG
Sbjct: 406 FLDFESG 412
>NP_543006
          Length = 170

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   EFLEFKSG 9
           E +EFKSG
Sbjct: 128 ENVEFKSG 135
>NP_071353
          Length = 1181

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1    MEFLEFK 7
            ++FLEFK
Sbjct: 1057 IDFLEFK 1063
>NP_064581
          Length = 855

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/9 (44%), Positives = 7/9 (77%)

Query: 1  MEFLEFKSG 9
          + ++EFK G
Sbjct: 39 LRYIEFKQG 47
>NP_061026
          Length = 387

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   EFLEFKSG 9
           E +EFKSG
Sbjct: 128 ENVEFKSG 135
>NP_001157936
          Length = 446

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   FLEFKSG 9
           FL+F+SG
Sbjct: 406 FLDFESG 412
>XP_006724905
          Length = 193

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2  EFLEFKSG 9
          EFL FK G
Sbjct: 89 EFLRFKWG 96
>XP_006724904
          Length = 223

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2   EFLEFKSG 9
           EFL FK G
Sbjct: 119 EFLRFKWG 126
>XP_006724899
          Length = 310

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2   EFLEFKSG 9
           EFL FK G
Sbjct: 206 EFLRFKWG 213
>XP_005260806
          Length = 281

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   EFLEFKSG 9
           E +EFKSG
Sbjct: 128 ENVEFKSG 135
>XP_005257627
          Length = 1013

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   MEFLEFK 7
           ++FLEFK
Sbjct: 889 IDFLEFK 895
>XP_006712770
          Length = 446

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   FLEFKSG 9
           FL+F+SG
Sbjct: 406 FLDFESG 412
>XP_006712325
          Length = 446

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   FLEFKSG 9
           FL+F+SG
Sbjct: 406 FLDFESG 412
>NP_001034597
          Length = 276

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   EFLEFKSG 9
           E +EFKSG
Sbjct: 128 ENVEFKSG 135
>NP_001474
          Length = 286

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   EFLEFK 7
           EFLEF+
Sbjct: 149 EFLEFR 154
>NP_001122074
          Length = 762

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   MEFLEFKS 8
           + +LEFKS
Sbjct: 213 LTYLEFKS 220
>NP_005730
          Length = 797

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   MEFLEFKS 8
           + +LEFKS
Sbjct: 213 LTYLEFKS 220
>NP_004068
          Length = 466

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   MEFLEFKSG 9
           M+F++ KSG
Sbjct: 458 MKFVDSKSG 466
>NP_066990
          Length = 269

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   MEFLEF 6
           ME+LEF
Sbjct: 216 MEYLEF 221
>NP_060124
          Length = 1589

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   EFLEFKS 8
           +FLEFK+
Sbjct: 671 KFLEFKA 677
>NP_002067
          Length = 552

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 1   MEFLEFKS 8
           ++FL++KS
Sbjct: 213 LDFLDYKS 220
>NP_071446
          Length = 279

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   EFLEFKSG 9
           +FL+FK G
Sbjct: 127 KFLQFKLG 134
>NP_060557
          Length = 674

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   EFLEFK 7
           +FLEFK
Sbjct: 668 QFLEFK 673
>NP_001129316
          Length = 398

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   EFLEFKSG 9
           + +EFKSG
Sbjct: 129 DHVEFKSG 136
>NP_001180236
          Length = 1589

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   EFLEFKS 8
           +FLEFK+
Sbjct: 671 KFLEFKA 677
>XP_006723598
          Length = 441

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   EFLEFKSG 9
           + +EFKSG
Sbjct: 172 DHVEFKSG 179
>XP_005254171
          Length = 746

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   MEFLEFKS 8
           + +LEFKS
Sbjct: 213 LTYLEFKS 220
>XP_005245370
          Length = 523

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1   MEFLEFKSG 9
           M FL  KSG
Sbjct: 376 MRFLNSKSG 384
>XP_006710825
          Length = 669

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   EFLEFK 7
           +FLEFK
Sbjct: 663 QFLEFK 668
>NP_001162023
          Length = 634

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   EFLEFK 7
           +FLEFK
Sbjct: 628 QFLEFK 633
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.325    0.143    0.395 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 470,938
Number of extensions: 662
Number of successful extensions: 48
Number of sequences better than 10000.0: 48
Number of HSP's gapped: 48
Number of HSP's successfully gapped: 48
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.9 bits)
S2: 28 (15.4 bits)

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