Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_006197                                                              17    2528
NP_002600                                                              17    2528
NP_001265723                                                           17    2528
NP_005963                                                              17    2528
NP_001265724                                                           17    2528
XP_005277701                                                           17    2528
XP_006717608                                                           17    2528
XP_005268521                                                           17    2528
XP_005265800                                                           17    2528
XP_006714103                                                           17    2528
XP_006714102                                                           17    2528
NP_001167535                                                           16    5631
NP_001167534                                                           16    5631
NP_001167536                                                           16    5631
NP_001167537                                                           16    5631
NP_002011                                                              16    5631
NP_891555                                                              16    5631
NP_056934                                                              16    5631
NP_075598                                                              16    5631
NP_075594                                                              16    5631
NP_075593                                                              16    5631
NP_002244                                                              16    5631
NP_001167538                                                           16    5631
XP_006716377                                                           16    5631
XP_006716376                                                           16    5631
XP_006716375                                                           16    5631
XP_006716374                                                           16    5631
XP_006716373                                                           16    5631
XP_006716372                                                           16    5631
XP_006716371                                                           16    5631
XP_006716370                                                           16    5631
XP_006716369                                                           16    5631
XP_006716368                                                           16    5631
XP_006716367                                                           16    5631
XP_006716366                                                           16    5631
NP_203752                                                              16    7355
NP_001139381                                                           16    7355
NP_079130                                                              16    7355
XP_006719216                                                           16    7355
XP_006712877                                                           16    7355
XP_006712876                                                           16    7355
XP_006712875                                                           16    7355
XP_006712874                                                           16    7355
XP_006712873                                                           16    7355
XP_006712872                                                           16    7355
XP_005246976                                                           16    7355
XP_005246975                                                           16    7355
NP_071328                                                              15    9605
>NP_006197
          Length = 1089

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 1   MEFLASK 7
           MEFLASK
Sbjct: 806 MEFLASK 812
>NP_002600
          Length = 1106

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 1   MEFLASK 7
           MEFLASK
Sbjct: 814 MEFLASK 820
>NP_001265723
          Length = 375

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   MEFLASKIV 9
           +EFLA K+V
Sbjct: 192 LEFLADKVV 200
>NP_005963
          Length = 375

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   MEFLASKIV 9
           +EFLA K+V
Sbjct: 192 LEFLADKVV 200
>NP_001265724
          Length = 375

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   MEFLASKIV 9
           +EFLA K+V
Sbjct: 192 LEFLADKVV 200
>XP_005277701
          Length = 375

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   MEFLASKIV 9
           +EFLA K+V
Sbjct: 192 LEFLADKVV 200
>XP_006717608
          Length = 375

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   MEFLASKIV 9
           +EFLA K+V
Sbjct: 192 LEFLADKVV 200
>XP_005268521
          Length = 1042

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 1   MEFLASK 7
           MEFLASK
Sbjct: 750 MEFLASK 756
>XP_005265800
          Length = 1089

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 1   MEFLASK 7
           MEFLASK
Sbjct: 806 MEFLASK 812
>XP_006714103
          Length = 1111

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 1   MEFLASK 7
           MEFLASK
Sbjct: 828 MEFLASK 834
>XP_006714102
          Length = 1114

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 1   MEFLASK 7
           MEFLASK
Sbjct: 831 MEFLASK 837
>NP_001167535
          Length = 812

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   MEFLASK 7
           ME+LASK
Sbjct: 601 MEYLASK 607
>NP_001167534
          Length = 820

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   MEFLASK 7
           ME+LASK
Sbjct: 609 MEYLASK 615
>NP_001167536
          Length = 820

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   MEFLASK 7
           ME+LASK
Sbjct: 609 MEYLASK 615
>NP_001167537
          Length = 733

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   MEFLASK 7
           ME+LASK
Sbjct: 522 MEYLASK 528
>NP_002011
          Length = 1298

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1    MEFLASK 7
            MEFLAS+
Sbjct: 1025 MEFLASR 1031
>NP_891555
          Length = 1363

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1    MEFLASK 7
            MEFLAS+
Sbjct: 1025 MEFLASR 1031
>NP_056934
          Length = 820

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   MEFLASK 7
           ME+LASK
Sbjct: 609 MEYLASK 615
>NP_075598
          Length = 822

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   MEFLASK 7
           ME+LASK
Sbjct: 611 MEYLASK 617
>NP_075594
          Length = 731

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   MEFLASK 7
           ME+LASK
Sbjct: 520 MEYLASK 526
>NP_075593
          Length = 733

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   MEFLASK 7
           ME+LASK
Sbjct: 522 MEYLASK 528
>NP_002244
          Length = 1356

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1    MEFLASK 7
            MEFLAS+
Sbjct: 1016 MEFLASR 1022
>NP_001167538
          Length = 853

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   MEFLASK 7
           ME+LASK
Sbjct: 642 MEYLASK 648
>XP_006716377
          Length = 741

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   MEFLASK 7
           ME+LASK
Sbjct: 520 MEYLASK 526
>XP_006716376
          Length = 741

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   MEFLASK 7
           ME+LASK
Sbjct: 520 MEYLASK 526
>XP_006716375
          Length = 743

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   MEFLASK 7
           ME+LASK
Sbjct: 522 MEYLASK 528
>XP_006716374
          Length = 743

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   MEFLASK 7
           ME+LASK
Sbjct: 522 MEYLASK 528
>XP_006716373
          Length = 743

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   MEFLASK 7
           ME+LASK
Sbjct: 522 MEYLASK 528
>XP_006716372
          Length = 824

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   MEFLASK 7
           ME+LASK
Sbjct: 603 MEYLASK 609
>XP_006716371
          Length = 830

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   MEFLASK 7
           ME+LASK
Sbjct: 609 MEYLASK 615
>XP_006716370
          Length = 830

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   MEFLASK 7
           ME+LASK
Sbjct: 609 MEYLASK 615
>XP_006716369
          Length = 830

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   MEFLASK 7
           ME+LASK
Sbjct: 609 MEYLASK 615
>XP_006716368
          Length = 832

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   MEFLASK 7
           ME+LASK
Sbjct: 611 MEYLASK 617
>XP_006716367
          Length = 832

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   MEFLASK 7
           ME+LASK
Sbjct: 611 MEYLASK 617
>XP_006716366
          Length = 832

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   MEFLASK 7
           ME+LASK
Sbjct: 611 MEYLASK 617
>NP_203752
          Length = 1861

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1   MEFLASKIV 9
           +++LASK+V
Sbjct: 495 VKYLASKVV 503
>NP_001139381
          Length = 1390

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1   MEFLASKIV 9
           +++LASK+V
Sbjct: 487 VKYLASKVV 495
>NP_079130
          Length = 500

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   EFLASKIV 9
           EFL SK++
Sbjct: 191 EFLTSKLI 198
>XP_006719216
          Length = 259

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   EFLASKIV 9
           EFL SK++
Sbjct: 191 EFLTSKLI 198
>XP_006712877
          Length = 1677

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1   MEFLASKIV 9
           +++LASK+V
Sbjct: 311 VKYLASKVV 319
>XP_006712876
          Length = 1783

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1   MEFLASKIV 9
           +++LASK+V
Sbjct: 417 VKYLASKVV 425
>XP_006712875
          Length = 1826

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1   MEFLASKIV 9
           +++LASK+V
Sbjct: 460 VKYLASKVV 468
>XP_006712874
          Length = 1836

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1   MEFLASKIV 9
           +++LASK+V
Sbjct: 470 VKYLASKVV 478
>XP_006712873
          Length = 1861

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1   MEFLASKIV 9
           +++LASK+V
Sbjct: 495 VKYLASKVV 503
>XP_006712872
          Length = 1861

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1   MEFLASKIV 9
           +++LASK+V
Sbjct: 495 VKYLASKVV 503
>XP_005246976
          Length = 1864

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1   MEFLASKIV 9
           +++LASK+V
Sbjct: 498 VKYLASKVV 506
>XP_005246975
          Length = 1871

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1   MEFLASKIV 9
           +++LASK+V
Sbjct: 505 VKYLASKVV 513
>NP_071328
          Length = 904

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   EFLASKIV 9
           +FLA KI+
Sbjct: 277 DFLAKKII 284
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.333    0.139    0.336 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 268,459
Number of extensions: 424
Number of successful extensions: 48
Number of sequences better than 10000.0: 48
Number of HSP's gapped: 48
Number of HSP's successfully gapped: 48
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.3 bits)
S2: 28 (15.4 bits)

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