Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_001895                                                              19     665
NP_001091680                                                           19     665
NP_001091679                                                           19     665
XP_006713048                                                           19     665
XP_006713047                                                           19     665
XP_006713046                                                           19     665
XP_005264943                                                           19     665
NP_001518                                                              18    1935
NP_001186091                                                           17    4312
NP_001186090                                                           17    4312
NP_006668                                                              17    4312
NP_001186089                                                           17    4312
NP_710142                                                              17    4312
NP_001257410                                                           17    4312
NP_002789                                                              17    4312
XP_006714837                                                           17    4312
XP_005264888                                                           17    4312
XP_006713016                                                           17    4312
XP_005264887                                                           17    4312
XP_006713015                                                           17    4312
XP_006713014                                                           17    4312
XP_006713013                                                           17    4312
XP_006713012                                                           17    4312
XP_006713011                                                           17    4312
XP_005264886                                                           17    4312
XP_006713010                                                           17    4312
XP_005264885                                                           17    4312
XP_005264884                                                           17    4312
XP_005264883                                                           17    4312
XP_005264882                                                           17    4312
XP_006713009                                                           17    4312
XP_005264881                                                           17    4312
XP_005264880                                                           17    4312
NP_001011705                                                           16    5631
NP_008946                                                              16    5631
NP_005898                                                              16    5631
NP_001274427                                                           16    5631
NP_115671                                                              16    5631
NP_996996                                                              16    5631
XP_006719824                                                           16    5631
XP_006719823                                                           16    5631
XP_005267043                                                           16    5631
NP_002791                                                              16    5631
NP_116253                                                              16    7355
NP_006690                                                              16    7355
NP_653272                                                              16    7355
NP_001171468                                                           16    7355
NP_001171467                                                           16    7355
XP_006719320                                                           16    7355
XP_006710365                                                           16    7355
NP_001611                                                              15    9605
NP_872433                                                              15    9605
XP_006718751                                                           15    9605
XP_006718750                                                           15    9605
XP_005274303                                                           15    9605
XP_006718749                                                           15    9605
XP_005274302                                                           15    9605
XP_005274301                                                           15    9605
XP_005274300                                                           15    9605
XP_005274299                                                           15    9605
XP_005274298                                                           15    9605
XP_005274297                                                           15    9605
XP_005269377                                                           15    9605
XP_005269376                                                           15    9605
>NP_001895
          Length = 781

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   MAVRFAGGL 9
           MAVR AGGL
Sbjct: 271 MAVRLAGGL 279
>NP_001091680
          Length = 781

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   MAVRFAGGL 9
           MAVR AGGL
Sbjct: 271 MAVRLAGGL 279
>NP_001091679
          Length = 781

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   MAVRFAGGL 9
           MAVR AGGL
Sbjct: 271 MAVRLAGGL 279
>XP_006713048
          Length = 720

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   MAVRFAGGL 9
           MAVR AGGL
Sbjct: 271 MAVRLAGGL 279
>XP_006713047
          Length = 774

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   MAVRFAGGL 9
           MAVR AGGL
Sbjct: 264 MAVRLAGGL 272
>XP_006713046
          Length = 774

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   MAVRFAGGL 9
           MAVR AGGL
Sbjct: 264 MAVRLAGGL 272
>XP_005264943
          Length = 781

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   MAVRFAGGL 9
           MAVR AGGL
Sbjct: 271 MAVRLAGGL 279
>NP_001518
          Length = 488

 Score = 17.7 bits (34), Expect =  1935
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   MAVRFAGGL 9
           MAV +AGGL
Sbjct: 132 MAVNWAGGL 140
>NP_001186091
          Length = 1372

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   VRFAGG 8
           VRFAGG
Sbjct: 154 VRFAGG 159
>NP_001186090
          Length = 1384

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   VRFAGG 8
           VRFAGG
Sbjct: 154 VRFAGG 159
>NP_006668
          Length = 1318

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   VRFAGG 8
           VRFAGG
Sbjct: 154 VRFAGG 159
>NP_001186089
          Length = 1419

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   VRFAGG 8
           VRFAGG
Sbjct: 154 VRFAGG 159
>NP_710142
          Length = 899

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   RFAGGL 9
           RFAGGL
Sbjct: 531 RFAGGL 536
>NP_001257410
          Length = 213

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1  MAVRFAGGL 9
          MAV+F GG+
Sbjct: 38 MAVQFDGGV 46
>NP_002789
          Length = 239

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1  MAVRFAGGL 9
          MAV+F GG+
Sbjct: 38 MAVQFDGGV 46
>XP_006714837
          Length = 628

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   VRFAGGL 9
           +R+AGGL
Sbjct: 567 IRYAGGL 573
>XP_005264888
          Length = 1268

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   VRFAGG 8
           VRFAGG
Sbjct: 139 VRFAGG 144
>XP_006713016
          Length = 1404

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   VRFAGG 8
           VRFAGG
Sbjct: 139 VRFAGG 144
>XP_005264887
          Length = 1322

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   VRFAGG 8
           VRFAGG
Sbjct: 154 VRFAGG 159
>XP_006713015
          Length = 1367

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   VRFAGG 8
           VRFAGG
Sbjct: 139 VRFAGG 144
>XP_006713014
          Length = 1368

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   VRFAGG 8
           VRFAGG
Sbjct: 139 VRFAGG 144
>XP_006713013
          Length = 1369

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   VRFAGG 8
           VRFAGG
Sbjct: 139 VRFAGG 144
>XP_006713012
          Length = 1373

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   VRFAGG 8
           VRFAGG
Sbjct: 154 VRFAGG 159
>XP_006713011
          Length = 1382

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   VRFAGG 8
           VRFAGG
Sbjct: 154 VRFAGG 159
>XP_005264886
          Length = 1386

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   VRFAGG 8
           VRFAGG
Sbjct: 154 VRFAGG 159
>XP_006713010
          Length = 1405

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   VRFAGG 8
           VRFAGG
Sbjct: 139 VRFAGG 144
>XP_005264885
          Length = 1406

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   VRFAGG 8
           VRFAGG
Sbjct: 139 VRFAGG 144
>XP_005264884
          Length = 1408

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   VRFAGG 8
           VRFAGG
Sbjct: 139 VRFAGG 144
>XP_005264883
          Length = 1421

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   VRFAGG 8
           VRFAGG
Sbjct: 154 VRFAGG 159
>XP_005264882
          Length = 1421

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   VRFAGG 8
           VRFAGG
Sbjct: 154 VRFAGG 159
>XP_006713009
          Length = 1422

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   VRFAGG 8
           VRFAGG
Sbjct: 154 VRFAGG 159
>XP_005264881
          Length = 1423

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   VRFAGG 8
           VRFAGG
Sbjct: 154 VRFAGG 159
>XP_005264880
          Length = 1423

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   VRFAGG 8
           VRFAGG
Sbjct: 154 VRFAGG 159
>NP_001011705
          Length = 333

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   VRFAGG 8
           +RFAGG
Sbjct: 123 IRFAGG 128
>NP_008946
          Length = 334

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   VRFAGG 8
           +RFAGG
Sbjct: 123 IRFAGG 128
>NP_005898
          Length = 653

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   VRFAGGL 9
           +RF GGL
Sbjct: 283 IRFVGGL 289
>NP_001274427
          Length = 478

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   AVRFAGGL 9
           +V+FAGG+
Sbjct: 214 SVQFAGGI 221
>NP_115671
          Length = 443

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   AVRFAGGL 9
           +V+FAGG+
Sbjct: 214 SVQFAGGI 221
>NP_996996
          Length = 494

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   AVRFAGGL 9
           +V+FAGG+
Sbjct: 214 SVQFAGGI 221
>XP_006719824
          Length = 217

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   VRFAGG 8
           +RFAGG
Sbjct: 123 IRFAGG 128
>XP_006719823
          Length = 218

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   VRFAGG 8
           +RFAGG
Sbjct: 123 IRFAGG 128
>XP_005267043
          Length = 736

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   VRFAGGL 9
           +RF GGL
Sbjct: 366 IRFVGGL 372
>NP_002791
          Length = 219

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1  MAVRFAGGL 9
          MAV F GG+
Sbjct: 24 MAVEFDGGV 32
>NP_116253
          Length = 312

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1  MAVRFAGGL 9
          M +RF GG+
Sbjct: 52 MELRFVGGV 60
>NP_006690
          Length = 641

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   VRFAGGL 9
           +RF GGL
Sbjct: 269 IRFIGGL 275
>NP_653272
          Length = 249

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2  AVRFAG 7
          AVRFAG
Sbjct: 80 AVRFAG 85
>NP_001171468
          Length = 249

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2  AVRFAG 7
          AVRFAG
Sbjct: 80 AVRFAG 85
>NP_001171467
          Length = 262

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2  AVRFAG 7
          AVRFAG
Sbjct: 93 AVRFAG 98
>XP_006719320
          Length = 249

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2  AVRFAG 7
          AVRFAG
Sbjct: 80 AVRFAG 85
>XP_006710365
          Length = 637

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   VRFAGGL 9
           +RF GGL
Sbjct: 269 IRFIGGL 275
>NP_001611
          Length = 5890

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4    RFAGGL 9
            +FAGGL
Sbjct: 5612 KFAGGL 5617
>NP_872433
          Length = 358

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1 MAVRFAGG 8
         MAV++ GG
Sbjct: 1 MAVKWTGG 8
>XP_006718751
          Length = 3489

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4    RFAGGL 9
            +FAGGL
Sbjct: 3211 KFAGGL 3216
>XP_006718750
          Length = 4001

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4    RFAGGL 9
            +FAGGL
Sbjct: 3723 KFAGGL 3728
>XP_005274303
          Length = 4506

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4    RFAGGL 9
            +FAGGL
Sbjct: 4228 KFAGGL 4233
>XP_006718749
          Length = 4596

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4    RFAGGL 9
            +FAGGL
Sbjct: 4318 KFAGGL 4323
>XP_005274302
          Length = 5438

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4    RFAGGL 9
            +FAGGL
Sbjct: 5160 KFAGGL 5165
>XP_005274301
          Length = 5762

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4    RFAGGL 9
            +FAGGL
Sbjct: 5484 KFAGGL 5489
>XP_005274300
          Length = 5762

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4    RFAGGL 9
            +FAGGL
Sbjct: 5484 KFAGGL 5489
>XP_005274299
          Length = 5762

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4    RFAGGL 9
            +FAGGL
Sbjct: 5484 KFAGGL 5489
>XP_005274298
          Length = 5890

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4    RFAGGL 9
            +FAGGL
Sbjct: 5612 KFAGGL 5617
>XP_005274297
          Length = 5890

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4    RFAGGL 9
            +FAGGL
Sbjct: 5612 KFAGGL 5617
>XP_005269377
          Length = 358

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1 MAVRFAGG 8
         MAV++ GG
Sbjct: 1 MAVKWTGG 8
>XP_005269376
          Length = 358

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1 MAVRFAGG 8
         MAV++ GG
Sbjct: 1 MAVKWTGG 8
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.339    0.150    0.444 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 469,888
Number of extensions: 286
Number of successful extensions: 64
Number of sequences better than 10000.0: 64
Number of HSP's gapped: 64
Number of HSP's successfully gapped: 64
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.4 bits)
S2: 28 (15.4 bits)

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