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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_003290                                                              18    1135
NP_001166028                                                           17    3301
NP_005886                                                              17    3301
XP_006719801                                                           17    3301
XP_006719800                                                           17    3301
XP_006719799                                                           17    3301
XP_005266219                                                           17    3301
NP_113584                                                              15    9605
NP_001129663                                                           15    9605
NP_056308                                                              15    9605
NP_001034663                                                           15    9605
XP_005262029                                                           15    9605
XP_005262028                                                           15    9605
XP_005262026                                                           15    9605
XP_006724637                                                           15    9605
XP_005262025                                                           15    9605
XP_005262024                                                           15    9605
XP_005262023                                                           15    9605
XP_005262022                                                           15    9605
XP_006710690                                                           15    9605

>NP_003290
          Length = 803

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   MAGSIRKEA 9
           MAGS RKEA
Sbjct: 541 MAGSSRKEA 549



>NP_001166028
          Length = 1134

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   GSIRKEA 9
           GS+RKEA
Sbjct: 114 GSVRKEA 120



>NP_005886
          Length = 1498

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   GSIRKEA 9
           GS+RKEA
Sbjct: 114 GSVRKEA 120



>XP_006719801
          Length = 1498

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   GSIRKEA 9
           GS+RKEA
Sbjct: 114 GSVRKEA 120



>XP_006719800
          Length = 1498

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   GSIRKEA 9
           GS+RKEA
Sbjct: 114 GSVRKEA 120



>XP_006719799
          Length = 1498

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   GSIRKEA 9
           GS+RKEA
Sbjct: 114 GSVRKEA 120



>XP_005266219
          Length = 1498

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   GSIRKEA 9
           GS+RKEA
Sbjct: 114 GSVRKEA 120



>NP_113584
          Length = 4374

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1    MAGSIRKE 8
            M G+IRK+
Sbjct: 4215 MTGAIRKQ 4222



>NP_001129663
          Length = 851

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   MAGSIRK 7
           MAG++RK
Sbjct: 192 MAGALRK 198



>NP_056308
          Length = 859

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   MAGSIRK 7
           MAG++RK
Sbjct: 192 MAGALRK 198



>NP_001034663
          Length = 626

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   GSIRKE 8
           GS+RKE
Sbjct: 602 GSVRKE 607



>XP_005262029
          Length = 4240

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1    MAGSIRKE 8
            M G+IRK+
Sbjct: 4081 MTGAIRKQ 4088



>XP_005262028
          Length = 4341

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1    MAGSIRKE 8
            M G+IRK+
Sbjct: 4182 MTGAIRKQ 4189



>XP_005262026
          Length = 4359

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1    MAGSIRKE 8
            M G+IRK+
Sbjct: 4200 MTGAIRKQ 4207



>XP_006724637
          Length = 4365

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1    MAGSIRKE 8
            M G+IRK+
Sbjct: 4206 MTGAIRKQ 4213



>XP_005262025
          Length = 4373

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1    MAGSIRKE 8
            M G+IRK+
Sbjct: 4214 MTGAIRKQ 4221



>XP_005262024
          Length = 4374

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1    MAGSIRKE 8
            M G+IRK+
Sbjct: 4215 MTGAIRKQ 4222



>XP_005262023
          Length = 4374

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1    MAGSIRKE 8
            M G+IRK+
Sbjct: 4215 MTGAIRKQ 4222



>XP_005262022
          Length = 4374

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1    MAGSIRKE 8
            M G+IRK+
Sbjct: 4215 MTGAIRKQ 4222



>XP_006710690
          Length = 624

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   GSIRKE 8
           GS+RKE
Sbjct: 602 GSVRKE 607



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.311    0.121    0.295 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 492,302
Number of extensions: 773
Number of successful extensions: 20
Number of sequences better than 10000.0: 20
Number of HSP's gapped: 20
Number of HSP's successfully gapped: 20
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.6 bits)
S2: 28 (15.4 bits)