Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_005742                                                              18    1935
NP_671714                                                              18    1935
XP_006715890                                                           18    1935
XP_005250160                                                           18    1935
XP_005250159                                                           18    1935
NP_001675                                                              16    5631
NP_001001396                                                           16    5631
NP_001135918                                                           16    5631
NP_065208                                                              16    5631
NP_036205                                                              16    5631
XP_005273533                                                           16    5631
NP_065210                                                              16    5631
NP_065209                                                              16    5631
NP_000028                                                              16    5631
NP_005532                                                              16    7355
NP_001017915                                                           16    7355
NP_689805                                                              16    7355
NP_057445                                                              16    7355
XP_006720129                                                           16    7355
XP_006720128                                                           16    7355
XP_006720127                                                           16    7355
XP_006720126                                                           16    7355
XP_005267433                                                           16    7355
XP_005267434                                                           16    7355
XP_005273686                                                           16    7355
XP_005273685                                                           16    7355
XP_005273684                                                           16    7355
XP_005273683                                                           16    7355
XP_005273682                                                           16    7355
XP_005273681                                                           16    7355
XP_005273679                                                           16    7355
XP_006711427                                                           16    7355
XP_005277457                                                           16    7355
XP_006711426                                                           16    7355
NP_006274                                                              16    7355
NP_055490                                                              15    9605
NP_055497                                                              15    9605
NP_003310                                                              15    9605
NP_597676                                                              15    9605
NP_596870                                                              15    9605
NP_001243779                                                           15    9605
NP_596869                                                              15    9605
NP_055486                                                              15    9605
XP_006716553                                                           15    9605
XP_006716552                                                           15    9605
XP_006716551                                                           15    9605
XP_006716550                                                           15    9605
XP_005249622                                                           15    9605
XP_005249621                                                           15    9605
XP_006712787                                                           15    9605
XP_006712786                                                           15    9605
XP_006711123                                                           15    9605
XP_006711122                                                           15    9605
XP_006711121                                                           15    9605
XP_006711120                                                           15    9605
XP_006711119                                                           15    9605
NP_001254479                                                           15    9605
NP_597681                                                              15    9605
>NP_005742
          Length = 3907

 Score = 17.7 bits (34), Expect =  1935
 Identities = 7/8 (87%), Positives = 8/8 (100%)

Query: 1   MAESQEAD 8
           MAESQEA+
Sbjct: 614 MAESQEAE 621
>NP_671714
          Length = 3899

 Score = 17.7 bits (34), Expect =  1935
 Identities = 7/8 (87%), Positives = 8/8 (100%)

Query: 1   MAESQEAD 8
           MAESQEA+
Sbjct: 614 MAESQEAE 621
>XP_006715890
          Length = 3860

 Score = 17.7 bits (34), Expect =  1935
 Identities = 7/8 (87%), Positives = 8/8 (100%)

Query: 1   MAESQEAD 8
           MAESQEA+
Sbjct: 614 MAESQEAE 621
>XP_005250160
          Length = 3939

 Score = 17.7 bits (34), Expect =  1935
 Identities = 7/8 (87%), Positives = 8/8 (100%)

Query: 1   MAESQEAD 8
           MAESQEA+
Sbjct: 614 MAESQEAE 621
>XP_005250159
          Length = 3952

 Score = 17.7 bits (34), Expect =  1935
 Identities = 7/8 (87%), Positives = 8/8 (100%)

Query: 1   MAESQEAD 8
           MAESQEA+
Sbjct: 614 MAESQEAE 621
>NP_001675
          Length = 1205

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1  MAESQEAD 8
          MAES+E D
Sbjct: 14 MAESREGD 21
>NP_001001396
          Length = 1170

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1  MAESQEAD 8
          MAES+E D
Sbjct: 14 MAESREGD 21
>NP_001135918
          Length = 1897

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2    AESQEADR 9
            AES +ADR
Sbjct: 1809 AESSQADR 1816
>NP_065208
          Length = 1856

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2    AESQEADR 9
            AES +ADR
Sbjct: 1768 AESSQADR 1775
>NP_036205
          Length = 541

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   MAESQEADR 9
           +A SQEAD+
Sbjct: 431 LAVSQEADK 439
>XP_005273533
          Length = 1921

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2    AESQEADR 9
            AES +ADR
Sbjct: 1809 AESSQADR 1816
>NP_065210
          Length = 1719

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2    AESQEADR 9
            AES +ADR
Sbjct: 1606 AESSQADR 1613
>NP_065209
          Length = 1881

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2    AESQEADR 9
            AES +ADR
Sbjct: 1768 AESSQADR 1775
>NP_000028
          Length = 1880

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2    AESQEADR 9
            AES +ADR
Sbjct: 1768 AESSQADR 1775
>NP_005532
          Length = 1188

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1    MAESQEADR 9
            + ++QEADR
Sbjct: 1061 LTKAQEADR 1069
>NP_001017915
          Length = 1189

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1    MAESQEADR 9
            + ++QEADR
Sbjct: 1062 LTKAQEADR 1070
>NP_689805
          Length = 975

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   AESQEAD 8
           AESQEA+
Sbjct: 683 AESQEAE 689
>NP_057445
          Length = 428

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1  MAESQEAD 8
          +AE QEAD
Sbjct: 31 LAELQEAD 38
>XP_006720129
          Length = 587

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   AESQEAD 8
           AESQEA+
Sbjct: 295 AESQEAE 301
>XP_006720128
          Length = 934

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   AESQEAD 8
           AESQEA+
Sbjct: 683 AESQEAE 689
>XP_006720127
          Length = 970

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   AESQEAD 8
           AESQEA+
Sbjct: 683 AESQEAE 689
>XP_006720126
          Length = 975

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   AESQEAD 8
           AESQEA+
Sbjct: 683 AESQEAE 689
>XP_005267433
          Length = 975

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   AESQEAD 8
           AESQEA+
Sbjct: 683 AESQEAE 689
>XP_005267434
          Length = 975

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   AESQEAD 8
           AESQEA+
Sbjct: 683 AESQEAE 689
>XP_005273686
          Length = 781

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3  ESQEAD 8
          ESQEAD
Sbjct: 57 ESQEAD 62
>XP_005273685
          Length = 789

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3  ESQEAD 8
          ESQEAD
Sbjct: 65 ESQEAD 70
>XP_005273684
          Length = 792

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   ESQEAD 8
           ESQEAD
Sbjct: 109 ESQEAD 114
>XP_005273683
          Length = 804

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   ESQEAD 8
           ESQEAD
Sbjct: 109 ESQEAD 114
>XP_005273682
          Length = 817

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3  ESQEAD 8
          ESQEAD
Sbjct: 93 ESQEAD 98
>XP_005273681
          Length = 821

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   ESQEAD 8
           ESQEAD
Sbjct: 109 ESQEAD 114
>XP_005273679
          Length = 833

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   ESQEAD 8
           ESQEAD
Sbjct: 109 ESQEAD 114
>XP_006711427
          Length = 228

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1  MAESQEAD 8
          +AE QEAD
Sbjct: 31 LAELQEAD 38
>XP_005277457
          Length = 271

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1  MAESQEAD 8
          +AE QEAD
Sbjct: 31 LAELQEAD 38
>XP_006711426
          Length = 385

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1  MAESQEAD 8
          +AE QEAD
Sbjct: 31 LAELQEAD 38
>NP_006274
          Length = 805

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3  ESQEAD 8
          ESQEAD
Sbjct: 93 ESQEAD 98
>NP_055490
          Length = 2017

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1    MAESQEADR 9
            +AES+EA R
Sbjct: 1560 VAESEEARR 1568
>NP_055497
          Length = 1047

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2   AESQEAD 8
           AES EAD
Sbjct: 159 AESDEAD 165
>NP_003310
          Length = 26926

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1    MAESQEA 7
            +AESQEA
Sbjct: 2585 VAESQEA 2591
>NP_597676
          Length = 27051

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1    MAESQEA 7
            +AESQEA
Sbjct: 2585 VAESQEA 2591
>NP_596870
          Length = 5604

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1    MAESQEA 7
            +AESQEA
Sbjct: 2631 VAESQEA 2637
>NP_001243779
          Length = 34350

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1    MAESQEA 7
            +AESQEA
Sbjct: 2631 VAESQEA 2637
>NP_596869
          Length = 33423

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1    MAESQEA 7
            +AESQEA
Sbjct: 2631 VAESQEA 2637
>NP_055486
          Length = 1083

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   ESQEADR 9
           ES+EAD+
Sbjct: 371 ESEEADK 377
>XP_006716553
          Length = 965

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2   AESQEAD 8
           AES EAD
Sbjct: 159 AESDEAD 165
>XP_006716552
          Length = 1038

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2   AESQEAD 8
           AES EAD
Sbjct: 159 AESDEAD 165
>XP_006716551
          Length = 1047

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2   AESQEAD 8
           AES EAD
Sbjct: 159 AESDEAD 165
>XP_006716550
          Length = 1047

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2   AESQEAD 8
           AES EAD
Sbjct: 159 AESDEAD 165
>XP_005249622
          Length = 1021

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   ESQEADR 9
           ES+EAD+
Sbjct: 371 ESEEADK 377
>XP_005249621
          Length = 1058

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   ESQEADR 9
           ES+EAD+
Sbjct: 346 ESEEADK 352
>XP_006712787
          Length = 33978

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1    MAESQEA 7
            +AESQEA
Sbjct: 2632 VAESQEA 2638
>XP_006712786
          Length = 35484

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1    MAESQEA 7
            +AESQEA
Sbjct: 2632 VAESQEA 2638
>XP_006711123
          Length = 1737

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1    MAESQEADR 9
            +AES+EA R
Sbjct: 1522 VAESEEARR 1530
>XP_006711122
          Length = 1918

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1    MAESQEADR 9
            +AES+EA R
Sbjct: 1522 VAESEEARR 1530
>XP_006711121
          Length = 1945

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1    MAESQEADR 9
            +AES+EA R
Sbjct: 1488 VAESEEARR 1496
>XP_006711120
          Length = 1972

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1    MAESQEADR 9
            +AES+EA R
Sbjct: 1522 VAESEEARR 1530
>XP_006711119
          Length = 1979

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1    MAESQEADR 9
            +AES+EA R
Sbjct: 1522 VAESEEARR 1530
>NP_001254479
          Length = 35991

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1    MAESQEA 7
            +AESQEA
Sbjct: 2631 VAESQEA 2637
>NP_597681
          Length = 27118

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1    MAESQEA 7
            +AESQEA
Sbjct: 2585 VAESQEA 2591
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.294    0.104    0.239 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 448,615
Number of extensions: 641
Number of successful extensions: 58
Number of sequences better than 10000.0: 58
Number of HSP's gapped: 58
Number of HSP's successfully gapped: 58
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 17 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.1 bits)
S2: 28 (15.4 bits)

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