Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_001268421                                                           20     299
NP_000170                                                              20     299
NP_001268422                                                           20     299
NP_001268423                                                           20     299
XP_005264328                                                           20     299
NP_009138                                                              17    3301
NP_001136438                                                           17    3301
NP_001144                                                              17    3301
XP_006722611                                                           17    3301
XP_006722610                                                           17    3301
XP_006712063                                                           17    3301
NP_055893                                                              17    4312
NP_008986                                                              16    5631
NP_004297                                                              16    5631
NP_060113                                                              16    5631
NP_061903                                                              16    5631
NP_001003954                                                           16    5631
NP_001180473                                                           16    5631
NP_001146                                                              16    5631
XP_006722025                                                           16    5631
XP_005257527                                                           16    5631
XP_005257526                                                           16    5631
XP_005257525                                                           16    5631
XP_005257524                                                           16    5631
XP_006717660                                                           16    5631
XP_005268489                                                           16    5631
XP_005248601                                                           16    5631
XP_006714716                                                           16    5631
NP_055120                                                              16    7355
NP_065962                                                              16    7355
NP_115618                                                              16    7355
XP_005250743                                                           16    7355
XP_006716234                                                           16    7355
XP_006713726                                                           16    7355
NP_001139241                                                           15    9605
NP_004619                                                              15    9605
NP_001122400                                                           15    9605
NP_001018098                                                           15    9605
NP_060154                                                              15    9605
NP_060550                                                              15    9605
NP_065872                                                              15    9605
NP_002855                                                              15    9605
NP_997646                                                              15    9605
NP_057480                                                              15    9605
XP_006720629                                                           15    9605
XP_005254498                                                           15    9605
XP_006720628                                                           15    9605
XP_005246589                                                           15    9605
XP_006712561                                                           15    9605
XP_006712265                                                           15    9605
XP_005263913                                                           15    9605
NP_002185                                                              15    9605
>NP_001268421
          Length = 1230

 Score = 20.4 bits (41), Expect =   299
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1    LYKFIKGA 8
            LYKFIKGA
Sbjct: 1156 LYKFIKGA 1163
>NP_000170
          Length = 1360

 Score = 20.4 bits (41), Expect =   299
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1    LYKFIKGA 8
            LYKFIKGA
Sbjct: 1286 LYKFIKGA 1293
>NP_001268422
          Length = 1058

 Score = 20.4 bits (41), Expect =   299
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1   LYKFIKGA 8
           LYKFIKGA
Sbjct: 984 LYKFIKGA 991
>NP_001268423
          Length = 1058

 Score = 20.4 bits (41), Expect =   299
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1   LYKFIKGA 8
           LYKFIKGA
Sbjct: 984 LYKFIKGA 991
>XP_005264328
          Length = 1261

 Score = 20.4 bits (41), Expect =   299
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1    LYKFIKGA 8
            LYKFIKGA
Sbjct: 1187 LYKFIKGA 1194
>NP_009138
          Length = 482

 Score = 16.9 bits (32), Expect =  3301
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   YKFIKG 7
           YKF+KG
Sbjct: 435 YKFVKG 440
>NP_001136438
          Length = 1923

 Score = 16.9 bits (32), Expect =  3301
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2    YKFIKGAS 9
            +KF+KGA+
Sbjct: 1865 FKFLKGAT 1872
>NP_001144
          Length = 321

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   LYKFIKG 7
           LY FIKG
Sbjct: 297 LYSFIKG 303
>XP_006722611
          Length = 1034

 Score = 16.9 bits (32), Expect =  3301
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   YKFIKGAS 9
           +KF+KGA+
Sbjct: 976 FKFLKGAT 983
>XP_006722610
          Length = 1967

 Score = 16.9 bits (32), Expect =  3301
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2    YKFIKGAS 9
            +KF+KGA+
Sbjct: 1909 FKFLKGAT 1916
>XP_006712063
          Length = 173

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   LYKFIKG 7
           LY FIKG
Sbjct: 149 LYSFIKG 155
>NP_055893
          Length = 1114

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   LYKFIKGAS 9
           L+K++KG S
Sbjct: 748 LFKYLKGPS 756
>NP_008986
          Length = 1390

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   KFIKGAS 9
           KFIKG S
Sbjct: 971 KFIKGVS 977
>NP_004297
          Length = 316

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   LYKFIKGA 8
           LYK +KGA
Sbjct: 254 LYKSMKGA 261
>NP_060113
          Length = 628

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   YKFIKGAS 9
           Y F++GAS
Sbjct: 605 YNFLQGAS 612
>NP_061903
          Length = 1369

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   LYKFIKGAS 9
           LY+ +KG S
Sbjct: 445 LYRLVKGQS 453
>NP_001003954
          Length = 357

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   LYKFIKGA 8
           LYK +KGA
Sbjct: 295 LYKSMKGA 302
>NP_001180473
          Length = 641

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   LYKFIKGA 8
           LYK +KGA
Sbjct: 576 LYKSMKGA 583
>NP_001146
          Length = 673

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   LYKFIKGA 8
           LYK +KGA
Sbjct: 608 LYKSMKGA 615
>XP_006722025
          Length = 464

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   YKFIKGAS 9
           Y F++GAS
Sbjct: 441 YNFLQGAS 448
>XP_005257527
          Length = 628

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   YKFIKGAS 9
           Y F++GAS
Sbjct: 605 YNFLQGAS 612
>XP_005257526
          Length = 630

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   YKFIKGAS 9
           Y F++GAS
Sbjct: 607 YNFLQGAS 614
>XP_005257525
          Length = 651

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   YKFIKGAS 9
           Y F++GAS
Sbjct: 628 YNFLQGAS 635
>XP_005257524
          Length = 653

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   YKFIKGAS 9
           Y F++GAS
Sbjct: 630 YNFLQGAS 637
>XP_006717660
          Length = 1369

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   KFIKGAS 9
           KFIKG S
Sbjct: 950 KFIKGVS 956
>XP_005268489
          Length = 667

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   LYKFIKGA 8
           LYK +KGA
Sbjct: 602 LYKSMKGA 609
>XP_005248601
          Length = 899

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   LYKFIKGAS 9
           LY+ +KG S
Sbjct: 445 LYRLVKGQS 453
>XP_006714716
          Length = 1318

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   LYKFIKGAS 9
           LY+ +KG S
Sbjct: 445 LYRLVKGQS 453
>NP_055120
          Length = 350

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2  YKFIKG 7
          YKFI+G
Sbjct: 70 YKFIQG 75
>NP_065962
          Length = 1894

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1    LYKFIK 6
            LYKF+K
Sbjct: 1446 LYKFLK 1451
>NP_115618
          Length = 1896

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1    LYKFIK 6
            LYKF+K
Sbjct: 1449 LYKFLK 1454
>XP_005250743
          Length = 1894

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1    LYKFIK 6
            LYKF+K
Sbjct: 1446 LYKFLK 1451
>XP_006716234
          Length = 1894

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1    LYKFIK 6
            LYKF+K
Sbjct: 1446 LYKFLK 1451
>XP_006713726
          Length = 1910

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1    LYKFIK 6
            LYKF+K
Sbjct: 1467 LYKFLK 1472
>NP_001139241
          Length = 903

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2   YKFIKGAS 9
           YK +KG S
Sbjct: 667 YKMVKGFS 674
>NP_004619
          Length = 940

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2   YKFIKGAS 9
           YK +KG S
Sbjct: 704 YKMVKGFS 711
>NP_001122400
          Length = 399

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 2   YKFIKGAS 9
           Y++IKG++
Sbjct: 159 YQYIKGSA 166
>NP_001018098
          Length = 3628

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/6 (66%), Positives = 6/6 (100%)

Query: 1    LYKFIK 6
            +YKF+K
Sbjct: 2557 IYKFVK 2562
>NP_060154
          Length = 3710

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/6 (66%), Positives = 6/6 (100%)

Query: 1    LYKFIK 6
            +YKF+K
Sbjct: 2514 IYKFVK 2519
>NP_060550
          Length = 3585

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/6 (66%), Positives = 6/6 (100%)

Query: 1    LYKFIK 6
            +YKF+K
Sbjct: 2514 IYKFVK 2519
>NP_065872
          Length = 3753

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/6 (66%), Positives = 6/6 (100%)

Query: 1    LYKFIK 6
            +YKF+K
Sbjct: 2557 IYKFVK 2562
>NP_002855
          Length = 1482

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 6/7 (85%)

Query: 1   LYKFIKG 7
           +Y F+KG
Sbjct: 662 IYSFLKG 668
>NP_997646
          Length = 594

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1  LYKFIK 6
          LYKF+K
Sbjct: 65 LYKFMK 70
>NP_057480
          Length = 466

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3  KFIKGAS 9
          KFIKG++
Sbjct: 54 KFIKGST 60
>XP_006720629
          Length = 342

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3  KFIKGAS 9
          KFIKG++
Sbjct: 54 KFIKGST 60
>XP_005254498
          Length = 466

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3  KFIKGAS 9
          KFIKG++
Sbjct: 54 KFIKGST 60
>XP_006720628
          Length = 466

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3  KFIKGAS 9
          KFIKG++
Sbjct: 54 KFIKGST 60
>XP_005246589
          Length = 399

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 2   YKFIKGAS 9
           Y++IKG++
Sbjct: 159 YQYIKGSA 166
>XP_006712561
          Length = 399

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 2   YKFIKGAS 9
           Y++IKG++
Sbjct: 159 YQYIKGSA 166
>XP_006712265
          Length = 593

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1  LYKFIK 6
          LYKF+K
Sbjct: 64 LYKFMK 69
>XP_005263913
          Length = 647

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1  LYKFIK 6
          LYKF+K
Sbjct: 65 LYKFMK 70
>NP_002185
          Length = 399

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 2   YKFIKGAS 9
           Y++IKG++
Sbjct: 159 YQYIKGSA 166
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.327    0.144    0.406 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 481,316
Number of extensions: 758
Number of successful extensions: 52
Number of sequences better than 10000.0: 52
Number of HSP's gapped: 52
Number of HSP's successfully gapped: 52
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.0 bits)
S2: 28 (15.4 bits)

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