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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_009057                                                              17    4312
NP_060656                                                              16    7355
NP_001273903                                                           16    7355
NP_003789                                                              16    7355
NP_116170                                                              16    7355
NP_008837                                                              16    7355
NP_001265167                                                           16    7355
XP_005252348                                                           16    7355
XP_005245376                                                           16    7355
XP_005245374                                                           16    7355
NP_076996                                                              15    9605
XP_006714561                                                           15    9605

>NP_009057
          Length = 806

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 9/9 (100%)

Query: 1   LVSQLLTII 9
           +VSQLLT++
Sbjct: 324 IVSQLLTLM 332



>NP_060656
          Length = 853

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   LVSQLLT 7
           L+SQLLT
Sbjct: 313 LISQLLT 319



>NP_001273903
          Length = 718

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1  LVSQLLTII 9
          LVSQ+ T+I
Sbjct: 44 LVSQITTLI 52



>NP_003789
          Length = 734

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1  LVSQLLTII 9
          LVSQ+ T+I
Sbjct: 44 LVSQITTLI 52



>NP_116170
          Length = 565

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   LVSQLLTII 9
           LVS LLT++
Sbjct: 119 LVSSLLTLL 127



>NP_008837
          Length = 565

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   LVSQLLTII 9
           LVS LLT++
Sbjct: 119 LVSSLLTLL 127



>NP_001265167
          Length = 541

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   LVSQLLTII 9
           LVS LLT++
Sbjct: 95  LVSSLLTLL 103



>XP_005252348
          Length = 732

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1  LVSQLLTII 9
          LVSQ+ T+I
Sbjct: 44 LVSQITTLI 52



>XP_005245376
          Length = 718

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   LVSQLLT 7
           L+SQLLT
Sbjct: 178 LISQLLT 184



>XP_005245374
          Length = 911

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   LVSQLLT 7
           L+SQLLT
Sbjct: 371 LISQLLT 377



>NP_076996
          Length = 662

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   LVSQLLTII 9
           L+SQ+LT +
Sbjct: 295 LISQMLTAL 303



>XP_006714561
          Length = 662

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   LVSQLLTII 9
           L+SQ+LT +
Sbjct: 295 LISQMLTAL 303



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.337    0.151    0.343 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 295,205
Number of extensions: 261
Number of successful extensions: 12
Number of sequences better than 10000.0: 12
Number of HSP's gapped: 12
Number of HSP's successfully gapped: 12
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.3 bits)
S2: 28 (15.4 bits)