Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_000312                                                              20     509
NP_115825                                                              16    5631
NP_005308                                                              16    5631
NP_001186363                                                           16    5631
NP_001160005                                                           16    5631
XP_005256247                                                           16    5631
XP_006710486                                                           16    5631
XP_006710485                                                           16    5631
XP_005270648                                                           16    5631
XP_005270647                                                           16    5631
NP_057185                                                              16    5631
NP_001136021                                                           16    5631
NP_113664                                                              16    5631
NP_077726                                                              16    5631
NP_000128                                                              16    7355
NP_001155155                                                           16    7355
NP_733764                                                              16    7355
NP_653273                                                              16    7355
NP_005420                                                              16    7355
NP_001161840                                                           16    7355
NP_065743                                                              16    7355
NP_001229761                                                           16    7355
NP_055829                                                              16    7355
NP_115765                                                              16    7355
XP_005253225                                                           16    7355
XP_006718409                                                           16    7355
XP_005253224                                                           16    7355
XP_005253223                                                           16    7355
NP_002264                                                              15    9605
NP_001243211                                                           15    9605
NP_001243222                                                           15    9605
NP_003949                                                              15    9605
NP_940953                                                              15    9605
NP_898901                                                              15    9605
XP_006715306                                                           15    9605
XP_006715305                                                           15    9605
XP_006715304                                                           15    9605
XP_006711367                                                           15    9605
XP_005273178                                                           15    9605
>NP_000312
          Length = 928

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   LVSIGKSFG 9
           LVSIG+SFG
Sbjct: 832 LVSIGESFG 840
>NP_115825
          Length = 858

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   LVSIGKSFG 9
           L+ IGK FG
Sbjct: 309 LMRIGKRFG 317
>NP_005308
          Length = 257

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2  VSIGKSFG 9
          +S+G SFG
Sbjct: 16 LSVGSSFG 23
>NP_001186363
          Length = 133

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1  LVSIGKSFG 9
          LVS+G  FG
Sbjct: 63 LVSLGTCFG 71
>NP_001160005
          Length = 704

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4  IGKSFG 9
          +GKSFG
Sbjct: 87 VGKSFG 92
>XP_005256247
          Length = 497

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   LVSIGKSFG 9
           LVSIG++ G
Sbjct: 107 LVSIGQNLG 115
>XP_006710486
          Length = 816

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   LVSIGKSFG 9
           L+ IGK FG
Sbjct: 309 LMRIGKRFG 317
>XP_006710485
          Length = 839

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   LVSIGKSFG 9
           L+ IGK FG
Sbjct: 309 LMRIGKRFG 317
>XP_005270648
          Length = 852

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   LVSIGKSFG 9
           L+ IGK FG
Sbjct: 309 LMRIGKRFG 317
>XP_005270647
          Length = 858

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   LVSIGKSFG 9
           L+ IGK FG
Sbjct: 309 LMRIGKRFG 317
>NP_057185
          Length = 180

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1  LVSIGKSFG 9
          LVS+G  FG
Sbjct: 63 LVSLGTCFG 71
>NP_001136021
          Length = 690

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4  IGKSFG 9
          +GKSFG
Sbjct: 87 VGKSFG 92
>NP_113664
          Length = 497

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   LVSIGKSFG 9
           LVSIG++ G
Sbjct: 107 LVSIGQNLG 115
>NP_077726
          Length = 724

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4  IGKSFG 9
          +GKSFG
Sbjct: 87 VGKSFG 92
>NP_000128
          Length = 419

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   IGKSFG 9
           +GKSFG
Sbjct: 251 LGKSFG 256
>NP_001155155
          Length = 1060

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   LVSIGKSF 8
           L+S+GKS+
Sbjct: 564 LISLGKSW 571
>NP_733764
          Length = 815

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   LVSIGKSF 8
           L+S+GKS+
Sbjct: 319 LISLGKSW 326
>NP_653273
          Length = 1139

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   LVSIGKSF 8
           L+S+GKS+
Sbjct: 643 LISLGKSW 650
>NP_005420
          Length = 419

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/8 (50%), Positives = 6/8 (75%)

Query: 2   VSIGKSFG 9
           + +GK FG
Sbjct: 138 IDVGKEFG 145
>NP_001161840
          Length = 558

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   SIGKSFG 9
           +IGK+FG
Sbjct: 389 AIGKAFG 395
>NP_065743
          Length = 558

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   SIGKSFG 9
           +IGK+FG
Sbjct: 389 AIGKAFG 395
>NP_001229761
          Length = 493

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   SIGKSFG 9
           S+G+SFG
Sbjct: 336 SLGESFG 342
>NP_055829
          Length = 430

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 6/9 (66%)

Query: 1   LVSIGKSFG 9
           +  IGK FG
Sbjct: 323 MTGIGKGFG 331
>NP_115765
          Length = 521

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   SIGKSFG 9
           S+G+SFG
Sbjct: 364 SLGESFG 370
>XP_005253225
          Length = 535

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   SIGKSFG 9
           S+G+SFG
Sbjct: 378 SLGESFG 384
>XP_006718409
          Length = 546

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   SIGKSFG 9
           S+G+SFG
Sbjct: 389 SLGESFG 395
>XP_005253224
          Length = 549

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   SIGKSFG 9
           S+G+SFG
Sbjct: 392 SLGESFG 398
>XP_005253223
          Length = 560

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   SIGKSFG 9
           S+G+SFG
Sbjct: 403 SLGESFG 409
>NP_002264
          Length = 483

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   SIGKSFG 9
           S+G SFG
Sbjct: 438 SLGSSFG 444
>NP_001243211
          Length = 511

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   SIGKSFG 9
           S+G SFG
Sbjct: 466 SLGSSFG 472
>NP_001243222
          Length = 483

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   SIGKSFG 9
           S+G SFG
Sbjct: 438 SLGSSFG 444
>NP_003949
          Length = 485

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 2  VSIGKSFG 9
          V++G+ FG
Sbjct: 38 VTVGRGFG 45
>NP_940953
          Length = 1907

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 3  SIGKSFG 9
          S+G++FG
Sbjct: 89 SVGRTFG 95
>NP_898901
          Length = 448

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 2  VSIGKSFG 9
          V++G+ FG
Sbjct: 38 VTVGRGFG 45
>XP_006715306
          Length = 334

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 2  VSIGKSFG 9
          V++G+ FG
Sbjct: 38 VTVGRGFG 45
>XP_006715305
          Length = 418

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 2  VSIGKSFG 9
          V++G+ FG
Sbjct: 38 VTVGRGFG 45
>XP_006715304
          Length = 455

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 2  VSIGKSFG 9
          V++G+ FG
Sbjct: 38 VTVGRGFG 45
>XP_006711367
          Length = 1872

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 3  SIGKSFG 9
          S+G++FG
Sbjct: 89 SVGRTFG 95
>XP_005273178
          Length = 1931

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 3  SIGKSFG 9
          S+G++FG
Sbjct: 89 SVGRTFG 95
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.321    0.145    0.396 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 493,036
Number of extensions: 663
Number of successful extensions: 39
Number of sequences better than 10000.0: 39
Number of HSP's gapped: 39
Number of HSP's successfully gapped: 39
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.8 bits)
S2: 28 (15.4 bits)

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