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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_006715                                                              18    1935
NP_005913                                                              18    1935
XP_005267046                                                           18    1935
XP_005267045                                                           18    1935
NP_077274                                                              16    5631
NP_001139555                                                           16    5631
NP_001139554                                                           16    5631
NP_001139528                                                           16    5631
NP_001127891                                                           15    9605
XP_005271302                                                           15    9605
XP_005271301                                                           15    9605
XP_005271298                                                           15    9605
NP_001127892                                                           15    9605
NP_003494                                                              15    9605

>NP_006715
          Length = 1558

 Score = 17.7 bits (34), Expect =  1935
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    LVSIAAELQ 9
            L SIAAELQ
Sbjct: 1185 LASIAAELQ 1193



>NP_005913
          Length = 1608

 Score = 17.7 bits (34), Expect =  1935
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    LVSIAAELQ 9
            L SIAAELQ
Sbjct: 1235 LASIAAELQ 1243



>XP_005267046
          Length = 1554

 Score = 17.7 bits (34), Expect =  1935
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    LVSIAAELQ 9
            L SIAAELQ
Sbjct: 1181 LASIAAELQ 1189



>XP_005267045
          Length = 1604

 Score = 17.7 bits (34), Expect =  1935
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    LVSIAAELQ 9
            L SIAAELQ
Sbjct: 1231 LASIAAELQ 1239



>NP_077274
          Length = 472

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 9/9 (100%)

Query: 1   LVSIAAELQ 9
           LVS+A+E+Q
Sbjct: 112 LVSLASEVQ 120



>NP_001139555
          Length = 399

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 9/9 (100%)

Query: 1  LVSIAAELQ 9
          LVS+A+E+Q
Sbjct: 39 LVSLASEVQ 47



>NP_001139554
          Length = 344

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 9/9 (100%)

Query: 1   LVSIAAELQ 9
           LVS+A+E+Q
Sbjct: 112 LVSLASEVQ 120



>NP_001139528
          Length = 399

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 9/9 (100%)

Query: 1  LVSIAAELQ 9
          LVS+A+E+Q
Sbjct: 39 LVSLASEVQ 47



>NP_001127891
          Length = 574

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   VSIAAELQ 9
           + IAAELQ
Sbjct: 99  IRIAAELQ 106



>XP_005271302
          Length = 368

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   VSIAAELQ 9
           + IAAELQ
Sbjct: 99  IRIAAELQ 106



>XP_005271301
          Length = 542

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   VSIAAELQ 9
           + IAAELQ
Sbjct: 99  IRIAAELQ 106



>XP_005271298
          Length = 574

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   VSIAAELQ 9
           + IAAELQ
Sbjct: 99  IRIAAELQ 106



>NP_001127892
          Length = 574

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   VSIAAELQ 9
           + IAAELQ
Sbjct: 99  IRIAAELQ 106



>NP_003494
          Length = 574

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   VSIAAELQ 9
           + IAAELQ
Sbjct: 99  IRIAAELQ 106



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.316    0.127    0.277 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 273,709
Number of extensions: 298
Number of successful extensions: 14
Number of sequences better than 10000.0: 14
Number of HSP's gapped: 14
Number of HSP's successfully gapped: 14
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.8 bits)
S2: 28 (15.4 bits)