Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_852668                                                              16    5631
NP_057184                                                              16    5631
NP_001001657                                                           16    5631
NP_078789                                                              16    7355
XP_005265541                                                           16    7355
XP_006713398                                                           16    7355
XP_006713397                                                           16    7355
XP_006713396                                                           16    7355
NP_060854                                                              15    9605
NP_055877                                                              15    9605
NP_001275656                                                           15    9605
NP_001275657                                                           15    9605
NP_004125                                                              15    9605
NP_001013725                                                           15    9605
NP_001034464                                                           15    9605
NP_077025                                                              15    9605
NP_001242911                                                           15    9605
NP_001242918                                                           15    9605
NP_954705                                                              15    9605
NP_001242913                                                           15    9605
NP_001242914                                                           15    9605
NP_001242916                                                           15    9605
NP_001242912                                                           15    9605
NP_001242917                                                           15    9605
NP_076932                                                              15    9605
NP_001242915                                                           15    9605
NP_663771                                                              15    9605
NP_006860                                                              15    9605
NP_001271240                                                           15    9605
NP_001271239                                                           15    9605
NP_001271238                                                           15    9605
NP_001271237                                                           15    9605
NP_619636                                                              15    9605
NP_001186506                                                           15    9605
XP_006723687                                                           15    9605
XP_006723686                                                           15    9605
XP_006723685                                                           15    9605
XP_006723684                                                           15    9605
XP_006723683                                                           15    9605
XP_005254450                                                           15    9605
XP_005254449                                                           15    9605
XP_006717794                                                           15    9605
XP_005269715                                                           15    9605
XP_006717793                                                           15    9605
XP_005269713                                                           15    9605
XP_006711960                                                           15    9605
XP_005263910                                                           15    9605
>NP_852668
          Length = 166

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2  VSEREGAF 9
          +SER+G F
Sbjct: 87 ISERKGGF 94
>NP_057184
          Length = 178

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   VSEREGAF 9
           +SER+G F
Sbjct: 99  ISERKGGF 106
>NP_001001657
          Length = 311

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   SEREGAF 9
           SEREG F
Sbjct: 266 SEREGKF 272
>NP_078789
          Length = 1478

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   SEREGA 8
           SEREGA
Sbjct: 849 SEREGA 854
>XP_005265541
          Length = 1080

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   SEREGA 8
           SEREGA
Sbjct: 849 SEREGA 854
>XP_006713398
          Length = 1478

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   SEREGA 8
           SEREGA
Sbjct: 849 SEREGA 854
>XP_006713397
          Length = 1478

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   SEREGA 8
           SEREGA
Sbjct: 849 SEREGA 854
>XP_006713396
          Length = 1478

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   SEREGA 8
           SEREGA
Sbjct: 849 SEREGA 854
>NP_060854
          Length = 307

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   EREGAF 9
           EREG+F
Sbjct: 213 EREGSF 218
>NP_055877
          Length = 1664

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   LVSEREGA 8
           LVS REG+
Sbjct: 155 LVSPREGS 162
>NP_001275656
          Length = 1400

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   LVSEREGA 8
           LVS REG+
Sbjct: 155 LVSPREGS 162
>NP_001275657
          Length = 1542

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1  LVSEREGA 8
          LVS REG+
Sbjct: 35 LVSPREGS 42
>NP_004125
          Length = 679

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   LVSEREGA 8
           + SEREG+
Sbjct: 655 MASEREGS 662
>NP_001013725
          Length = 1649

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1    LVSEREGA 8
            LVS++EG+
Sbjct: 1453 LVSDKEGS 1460
>NP_001034464
          Length = 317

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   SEREGAF 9
           +EREG F
Sbjct: 178 TEREGGF 184
>NP_077025
          Length = 345

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   SEREGAF 9
           +EREG F
Sbjct: 206 TEREGGF 212
>NP_001242911
          Length = 247

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   EREGAF 9
           E+EGAF
Sbjct: 185 EKEGAF 190
>NP_001242918
          Length = 197

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   EREGAF 9
           E+EGAF
Sbjct: 135 EKEGAF 140
>NP_954705
          Length = 268

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   EREGAF 9
           E+EGAF
Sbjct: 206 EKEGAF 211
>NP_001242913
          Length = 223

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   EREGAF 9
           E+EGAF
Sbjct: 161 EKEGAF 166
>NP_001242914
          Length = 285

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   EREGAF 9
           E+EGAF
Sbjct: 223 EKEGAF 228
>NP_001242916
          Length = 221

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   EREGAF 9
           E+EGAF
Sbjct: 159 EKEGAF 164
>NP_001242912
          Length = 247

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   EREGAF 9
           E+EGAF
Sbjct: 185 EKEGAF 190
>NP_001242917
          Length = 221

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   EREGAF 9
           E+EGAF
Sbjct: 159 EKEGAF 164
>NP_076932
          Length = 271

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   EREGAF 9
           E+EGAF
Sbjct: 209 EKEGAF 214
>NP_001242915
          Length = 245

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   EREGAF 9
           E+EGAF
Sbjct: 183 EKEGAF 188
>NP_663771
          Length = 471

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   EREGAF 9
           +REGAF
Sbjct: 420 DREGAF 425
>NP_006860
          Length = 374

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   LVSEREGA 8
           +++EREGA
Sbjct: 152 VLTEREGA 159
>NP_001271240
          Length = 279

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1  LVSEREGA 8
          +++EREGA
Sbjct: 57 VLTEREGA 64
>NP_001271239
          Length = 302

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1  LVSEREGA 8
          +++EREGA
Sbjct: 80 VLTEREGA 87
>NP_001271238
          Length = 302

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1  LVSEREGA 8
          +++EREGA
Sbjct: 80 VLTEREGA 87
>NP_001271237
          Length = 385

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   LVSEREGA 8
           +++EREGA
Sbjct: 163 VLTEREGA 170
>NP_619636
          Length = 760

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2   VSEREGA 8
           VSE EGA
Sbjct: 127 VSENEGA 133
>NP_001186506
          Length = 511

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   EREGAF 9
           EREG+F
Sbjct: 417 EREGSF 422
>XP_006723687
          Length = 188

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3  SEREGAF 9
          +EREG F
Sbjct: 49 TEREGGF 55
>XP_006723686
          Length = 188

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3  SEREGAF 9
          +EREG F
Sbjct: 49 TEREGGF 55
>XP_006723685
          Length = 188

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3  SEREGAF 9
          +EREG F
Sbjct: 49 TEREGGF 55
>XP_006723684
          Length = 247

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   SEREGAF 9
           +EREG F
Sbjct: 206 TEREGGF 212
>XP_006723683
          Length = 247

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   SEREGAF 9
           +EREG F
Sbjct: 206 TEREGGF 212
>XP_005254450
          Length = 1505

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1    LVSEREGA 8
            LVS++EG+
Sbjct: 1453 LVSDKEGS 1460
>XP_005254449
          Length = 1626

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1    LVSEREGA 8
            LVS++EG+
Sbjct: 1453 LVSDKEGS 1460
>XP_006717794
          Length = 1194

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   LVSEREGA 8
           LVS REG+
Sbjct: 155 LVSPREGS 162
>XP_005269715
          Length = 1230

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   LVSEREGA 8
           LVS REG+
Sbjct: 155 LVSPREGS 162
>XP_006717793
          Length = 1603

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   LVSEREGA 8
           LVS REG+
Sbjct: 155 LVSPREGS 162
>XP_005269713
          Length = 1646

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   LVSEREGA 8
           LVS REG+
Sbjct: 155 LVSPREGS 162
>XP_006711960
          Length = 261

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   EREGAF 9
           E+EGAF
Sbjct: 199 EKEGAF 204
>XP_005263910
          Length = 261

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   EREGAF 9
           E+EGAF
Sbjct: 199 EKEGAF 204
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.317    0.136    0.352 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 565,908
Number of extensions: 749
Number of successful extensions: 47
Number of sequences better than 10000.0: 47
Number of HSP's gapped: 47
Number of HSP's successfully gapped: 47
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.7 bits)
S2: 28 (15.4 bits)

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