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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_919047                                                              19     665
XP_005250598                                                           19     665
NP_071378                                                              17    4312
XP_005260563                                                           17    4312
XP_005260561                                                           17    4312
NP_002408                                                              15    9605
NP_001139438                                                           15    9605
NP_001269677                                                           15    9605
XP_006717927                                                           15    9605
XP_005252232                                                           15    9605
XP_005252231                                                           15    9605
XP_005252230                                                           15    9605
XP_006717332                                                           15    9605
XP_005252226                                                           15    9605
XP_005252224                                                           15    9605
XP_006717330                                                           15    9605
XP_006717329                                                           15    9605
XP_006717328                                                           15    9605
XP_006717327                                                           15    9605

>NP_919047
          Length = 520

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   LVMGKVSQT 9
           LVMGKVS+T
Sbjct: 360 LVMGKVSRT 368



>XP_005250598
          Length = 457

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   LVMGKVSQT 9
           LVMGKVS+T
Sbjct: 297 LVMGKVSRT 305



>NP_071378
          Length = 1342

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   LVMGKVSQT 9
           + MGKVS+T
Sbjct: 401 VAMGKVSRT 409



>XP_005260563
          Length = 1166

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   LVMGKVSQT 9
           + MGKVS+T
Sbjct: 225 VAMGKVSRT 233



>XP_005260561
          Length = 1341

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   LVMGKVSQT 9
           + MGKVS+T
Sbjct: 400 VAMGKVSRT 408



>NP_002408
          Length = 3256

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1    LVMGKVSQT 9
            L +GK++QT
Sbjct: 1647 LAVGKLTQT 1655




 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1    LVMGKVSQT 9
            L +GK++QT
Sbjct: 2856 LAVGKLTQT 2864



>NP_001139438
          Length = 2896

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1    LVMGKVSQT 9
            L +GK++QT
Sbjct: 1287 LAVGKLTQT 1295




 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1    LVMGKVSQT 9
            L +GK++QT
Sbjct: 2496 LAVGKLTQT 2504



>NP_001269677
          Length = 805

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   MGKVSQT 9
           MGK+S+T
Sbjct: 316 MGKLSET 322



>XP_006717927
          Length = 2406

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   LVMGKVSQT 9
           L +GK++QT
Sbjct: 797 LAVGKLTQT 805




 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1    LVMGKVSQT 9
            L +GK++QT
Sbjct: 2006 LAVGKLTQT 2014



>XP_005252232
          Length = 781

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   MGKVSQT 9
           MGK+S+T
Sbjct: 292 MGKLSET 298



>XP_005252231
          Length = 784

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   MGKVSQT 9
           MGK+S+T
Sbjct: 295 MGKLSET 301



>XP_005252230
          Length = 785

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   MGKVSQT 9
           MGK+S+T
Sbjct: 296 MGKLSET 302



>XP_006717332
          Length = 794

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   MGKVSQT 9
           MGK+S+T
Sbjct: 305 MGKLSET 311



>XP_005252226
          Length = 806

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   MGKVSQT 9
           MGK+S+T
Sbjct: 317 MGKLSET 323



>XP_005252224
          Length = 819

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   MGKVSQT 9
           MGK+S+T
Sbjct: 330 MGKLSET 336



>XP_006717330
          Length = 822

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   MGKVSQT 9
           MGK+S+T
Sbjct: 333 MGKLSET 339



>XP_006717329
          Length = 826

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   MGKVSQT 9
           MGK+S+T
Sbjct: 337 MGKLSET 343



>XP_006717328
          Length = 845

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   MGKVSQT 9
           MGK+S+T
Sbjct: 356 MGKLSET 362



>XP_006717327
          Length = 847

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   MGKVSQT 9
           MGK+S+T
Sbjct: 358 MGKLSET 364



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.315    0.125    0.307 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 323,713
Number of extensions: 224
Number of successful extensions: 22
Number of sequences better than 10000.0: 19
Number of HSP's gapped: 22
Number of HSP's successfully gapped: 22
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.7 bits)
S2: 28 (15.4 bits)