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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_005742                                                              19     869
NP_671714                                                              19     869
XP_006715890                                                           19     869
XP_005250160                                                           19     869
XP_005250159                                                           19     869
NP_001634                                                              17    4312
NP_001073286                                                           16    7355
NP_000201                                                              16    7355
NP_003290                                                              16    7355
XP_006712574                                                           16    7355
XP_006712573                                                           16    7355
NP_115642                                                              15    9605
NP_775321                                                              15    9605
NP_001106649                                                           15    9605
NP_619728                                                              15    9605
NP_006590                                                              15    9605
NP_619727                                                              15    9605
NP_775322                                                              15    9605
XP_005255834                                                           15    9605
XP_006721188                                                           15    9605

>NP_005742
          Length = 3907

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    LVKNAGTQI 9
            LVKNAG QI
Sbjct: 2798 LVKNAGIQI 2806



>NP_671714
          Length = 3899

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    LVKNAGTQI 9
            LVKNAG QI
Sbjct: 2790 LVKNAGIQI 2798



>XP_006715890
          Length = 3860

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    LVKNAGTQI 9
            LVKNAG QI
Sbjct: 2751 LVKNAGIQI 2759



>XP_005250160
          Length = 3939

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    LVKNAGTQI 9
            LVKNAG QI
Sbjct: 2830 LVKNAGIQI 2838



>XP_005250159
          Length = 3952

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    LVKNAGTQI 9
            LVKNAG QI
Sbjct: 2843 LVKNAGIQI 2851



>NP_001634
          Length = 100

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1  LVKNAGTQI 9
          L+K AGT++
Sbjct: 75 LIKKAGTEL 83



>NP_001073286
          Length = 1091

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   NAGTQI 9
           NAGTQ+
Sbjct: 992 NAGTQV 997



>NP_000201
          Length = 1073

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   NAGTQI 9
           NAGTQ+
Sbjct: 992 NAGTQV 997



>NP_003290
          Length = 803

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   LVKNAGT 7
           LVKN GT
Sbjct: 159 LVKNLGT 165



>XP_006712574
          Length = 1058

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   NAGTQI 9
           NAGTQ+
Sbjct: 977 NAGTQV 982



>XP_006712573
          Length = 1076

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   NAGTQI 9
           NAGTQ+
Sbjct: 977 NAGTQV 982



>NP_115642
          Length = 1984

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2   VKNAGTQI 9
           +KN GT I
Sbjct: 767 IKNPGTDI 774



>NP_775321
          Length = 1455

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   VKNAGTQ 8
           V+N+GTQ
Sbjct: 957 VQNSGTQ 963



>NP_001106649
          Length = 1548

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2    VKNAGTQ 8
            V+N+GTQ
Sbjct: 1050 VQNSGTQ 1056



>NP_619728
          Length = 1455

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   VKNAGTQ 8
           V+N+GTQ
Sbjct: 957 VQNSGTQ 963



>NP_006590
          Length = 1531

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2    VKNAGTQ 8
            V+N+GTQ
Sbjct: 1033 VQNSGTQ 1039



>NP_619727
          Length = 1549

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2    VKNAGTQ 8
            V+N+GTQ
Sbjct: 1051 VQNSGTQ 1057



>NP_775322
          Length = 1455

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   VKNAGTQ 8
           V+N+GTQ
Sbjct: 957 VQNSGTQ 963



>XP_005255834
          Length = 1264

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   VKNAGTQ 8
           V+N+GTQ
Sbjct: 766 VQNSGTQ 772



>XP_006721188
          Length = 1484

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   VKNAGTQ 8
           V+N+GTQ
Sbjct: 986 VQNSGTQ 992



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.313    0.130    0.331 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 472,935
Number of extensions: 579
Number of successful extensions: 20
Number of sequences better than 10000.0: 20
Number of HSP's gapped: 20
Number of HSP's successfully gapped: 20
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.6 bits)
S2: 28 (15.4 bits)