Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_001261                                                              20     509
NP_001262                                                              20     509
XP_005271924                                                           20     509
XP_005271923                                                           20     509
NP_000543                                                              16    7355
NP_612377                                                              15    9605
NP_001008779                                                           15    9605
NP_877433                                                              15    9605
NP_001136106                                                           15    9605
NP_001269399                                                           15    9605
NP_001275743                                                           15    9605
NP_115826                                                              15    9605
NP_071741                                                              15    9605
NP_001269402                                                           15    9605
NP_055434                                                              15    9605
XP_006716698                                                           15    9605
XP_006716697                                                           15    9605
XP_006716696                                                           15    9605
XP_006716695                                                           15    9605
XP_006716694                                                           15    9605
XP_006716693                                                           15    9605
XP_006716692                                                           15    9605
XP_006716691                                                           15    9605
XP_006716690                                                           15    9605
XP_006716689                                                           15    9605
XP_006714035                                                           15    9605
XP_006714034                                                           15    9605
>NP_001261
          Length = 1710

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   LVIQRMDTS 9
           LVIQRMDT+
Sbjct: 935 LVIQRMDTT 943
>NP_001262
          Length = 1828

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   LVIQRMDTS 9
           LVIQRMDT+
Sbjct: 938 LVIQRMDTT 946
>XP_005271924
          Length = 1710

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   LVIQRMDTS 9
           LVIQRMDT+
Sbjct: 935 LVIQRMDTT 943
>XP_005271923
          Length = 1798

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   LVIQRMDTS 9
           LVIQRMDT+
Sbjct: 935 LVIQRMDTT 943
>NP_000543
          Length = 2813

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2    VIQRMD 7
            VIQRMD
Sbjct: 1524 VIQRMD 1529
>NP_612377
          Length = 878

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   VIQRMDTS 9
           VIQ +DTS
Sbjct: 271 VIQLLDTS 278
>NP_001008779
          Length = 286

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1  LVIQRMD 7
          LVIQR D
Sbjct: 64 LVIQRQD 70
>NP_877433
          Length = 313

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1  LVIQRMD 7
          LVIQR D
Sbjct: 64 LVIQRQD 70
>NP_001136106
          Length = 313

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1  LVIQRMD 7
          LVIQR D
Sbjct: 64 LVIQRQD 70
>NP_001269399
          Length = 956

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   LVIQRMD 7
           L +QRMD
Sbjct: 644 LTVQRMD 650
>NP_001275743
          Length = 1632

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2    VIQRMD 7
            V+QRMD
Sbjct: 1273 VVQRMD 1278
>NP_115826
          Length = 1641

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2    VIQRMD 7
            V+QRMD
Sbjct: 1282 VVQRMD 1287
>NP_071741
          Length = 1015

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   LVIQRMDTS 9
           L+I+ +DTS
Sbjct: 407 LIIKSLDTS 415
>NP_001269402
          Length = 765

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   LVIQRMD 7
           L +QRMD
Sbjct: 644 LTVQRMD 650
>NP_055434
          Length = 956

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   LVIQRMD 7
           L +QRMD
Sbjct: 644 LTVQRMD 650
>XP_006716698
          Length = 1481

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2    VIQRMD 7
            V+QRMD
Sbjct: 1282 VVQRMD 1287
>XP_006716697
          Length = 1578

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2    VIQRMD 7
            V+QRMD
Sbjct: 1282 VVQRMD 1287
>XP_006716696
          Length = 1585

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2    VIQRMD 7
            V+QRMD
Sbjct: 1214 VVQRMD 1219
>XP_006716695
          Length = 1585

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2    VIQRMD 7
            V+QRMD
Sbjct: 1214 VVQRMD 1219
>XP_006716694
          Length = 1632

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2    VIQRMD 7
            V+QRMD
Sbjct: 1273 VVQRMD 1278
>XP_006716693
          Length = 1644

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2    VIQRMD 7
            V+QRMD
Sbjct: 1273 VVQRMD 1278
>XP_006716692
          Length = 1644

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2    VIQRMD 7
            V+QRMD
Sbjct: 1273 VVQRMD 1278
>XP_006716691
          Length = 1653

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2    VIQRMD 7
            V+QRMD
Sbjct: 1282 VVQRMD 1287
>XP_006716690
          Length = 1653

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2    VIQRMD 7
            V+QRMD
Sbjct: 1282 VVQRMD 1287
>XP_006716689
          Length = 1653

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2    VIQRMD 7
            V+QRMD
Sbjct: 1282 VVQRMD 1287
>XP_006714035
          Length = 941

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   LVIQRMDTS 9
           L+I+ +DTS
Sbjct: 407 LIIKSLDTS 415
>XP_006714034
          Length = 941

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   LVIQRMDTS 9
           L+I+ +DTS
Sbjct: 407 LIIKSLDTS 415
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.328    0.134    0.328 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 382,305
Number of extensions: 514
Number of successful extensions: 27
Number of sequences better than 10000.0: 27
Number of HSP's gapped: 27
Number of HSP's successfully gapped: 27
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.2 bits)
S2: 28 (15.4 bits)

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