Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_004251                                                              20     509
NP_714921                                                              18    1482
NP_006188                                                              17    3301
XP_006716412                                                           17    3301
XP_005273578                                                           17    3301
XP_005273577                                                           17    3301
XP_005273576                                                           17    3301
XP_006716411                                                           17    3301
XP_006716410                                                           17    3301
XP_006716409                                                           17    3301
XP_005273569                                                           17    3301
XP_005273566                                                           17    3301
XP_006716408                                                           17    3301
XP_006716407                                                           17    3301
XP_006716406                                                           17    3301
XP_006716405                                                           17    3301
XP_006716404                                                           17    3301
XP_006716403                                                           17    3301
XP_006716402                                                           17    3301
XP_006716401                                                           17    3301
XP_006716400                                                           17    3301
XP_006716399                                                           17    3301
NP_006022                                                              17    4312
NP_056270                                                              17    4312
XP_005261187                                                           17    4312
XP_005261186                                                           17    4312
XP_005261185                                                           17    4312
XP_005261184                                                           17    4312
XP_005261183                                                           17    4312
XP_005261182                                                           17    4312
XP_005261181                                                           17    4312
XP_006714963                                                           17    4312
XP_006714962                                                           17    4312
XP_005266010                                                           17    4312
NP_115543                                                              16    7355
XP_006721041                                                           16    7355
XP_006721040                                                           16    7355
XP_005255739                                                           16    7355
NP_001138508                                                           15    9605
NP_031383                                                              15    9605
NP_542376                                                              15    9605
NP_001035502                                                           15    9605
NP_001035501                                                           15    9605
NP_004361                                                              15    9605
NP_976044                                                              15    9605
NP_001274320                                                           15    9605
NP_001035500                                                           15    9605
NP_002310                                                              15    9605
NP_001276863                                                           15    9605
NP_001008395                                                           15    9605
XP_005250404                                                           15    9605
XP_005250403                                                           15    9605
XP_006715397                                                           15    9605
NP_001135389                                                           15    9605
>NP_004251
          Length = 1208

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    LVEFNELAF 9
            LVEF+ELAF
Sbjct: 1027 LVEFSELAF 1035
>NP_714921
          Length = 680

 Score = 18.1 bits (35), Expect =  1482
 Identities = 6/9 (66%), Positives = 9/9 (100%)

Query: 1   LVEFNELAF 9
           ++EFNEL+F
Sbjct: 107 VMEFNELSF 115
>NP_006188
          Length = 2024

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2    VEFNELAF 9
            V FNELAF
Sbjct: 1679 VLFNELAF 1686
>XP_006716412
          Length = 1970

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2    VEFNELAF 9
            V FNELAF
Sbjct: 1625 VLFNELAF 1632
>XP_005273578
          Length = 2016

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2    VEFNELAF 9
            V FNELAF
Sbjct: 1671 VLFNELAF 1678
>XP_005273577
          Length = 2048

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2    VEFNELAF 9
            V FNELAF
Sbjct: 1703 VLFNELAF 1710
>XP_005273576
          Length = 2048

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2    VEFNELAF 9
            V FNELAF
Sbjct: 1703 VLFNELAF 1710
>XP_006716411
          Length = 2064

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2    VEFNELAF 9
            V FNELAF
Sbjct: 1719 VLFNELAF 1726
>XP_006716410
          Length = 2072

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2    VEFNELAF 9
            V FNELAF
Sbjct: 1727 VLFNELAF 1734
>XP_006716409
          Length = 2076

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2    VEFNELAF 9
            V FNELAF
Sbjct: 1731 VLFNELAF 1738
>XP_005273569
          Length = 2086

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2    VEFNELAF 9
            V FNELAF
Sbjct: 1741 VLFNELAF 1748
>XP_005273566
          Length = 2087

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2    VEFNELAF 9
            V FNELAF
Sbjct: 1742 VLFNELAF 1749
>XP_006716408
          Length = 2117

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2    VEFNELAF 9
            V FNELAF
Sbjct: 1772 VLFNELAF 1779
>XP_006716407
          Length = 2126

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2    VEFNELAF 9
            V FNELAF
Sbjct: 1781 VLFNELAF 1788
>XP_006716406
          Length = 2131

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2    VEFNELAF 9
            V FNELAF
Sbjct: 1786 VLFNELAF 1793
>XP_006716405
          Length = 2138

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2    VEFNELAF 9
            V FNELAF
Sbjct: 1793 VLFNELAF 1800
>XP_006716404
          Length = 2142

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2    VEFNELAF 9
            V FNELAF
Sbjct: 1797 VLFNELAF 1804
>XP_006716403
          Length = 2146

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2    VEFNELAF 9
            V FNELAF
Sbjct: 1801 VLFNELAF 1808
>XP_006716402
          Length = 2149

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2    VEFNELAF 9
            V FNELAF
Sbjct: 1804 VLFNELAF 1811
>XP_006716401
          Length = 2150

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2    VEFNELAF 9
            V FNELAF
Sbjct: 1805 VLFNELAF 1812
>XP_006716400
          Length = 2150

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2    VEFNELAF 9
            V FNELAF
Sbjct: 1805 VLFNELAF 1812
>XP_006716399
          Length = 2150

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2    VEFNELAF 9
            V FNELAF
Sbjct: 1805 VLFNELAF 1812
>NP_006022
          Length = 3336

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 1    LVEFNELA 8
            L EFNELA
Sbjct: 1540 LDEFNELA 1547
>NP_056270
          Length = 557

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   VEFNELA 8
           VEFN+LA
Sbjct: 300 VEFNQLA 306
>XP_005261187
          Length = 2706

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 1    LVEFNELA 8
            L EFNELA
Sbjct: 1551 LDEFNELA 1558
>XP_005261186
          Length = 3139

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 1    LVEFNELA 8
            L EFNELA
Sbjct: 1422 LDEFNELA 1429
>XP_005261185
          Length = 3229

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 1    LVEFNELA 8
            L EFNELA
Sbjct: 1433 LDEFNELA 1440
>XP_005261184
          Length = 3258

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 1    LVEFNELA 8
            L EFNELA
Sbjct: 1551 LDEFNELA 1558
>XP_005261183
          Length = 3268

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 1    LVEFNELA 8
            L EFNELA
Sbjct: 1551 LDEFNELA 1558
>XP_005261182
          Length = 3346

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 1    LVEFNELA 8
            L EFNELA
Sbjct: 1550 LDEFNELA 1557
>XP_005261181
          Length = 3347

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 1    LVEFNELA 8
            L EFNELA
Sbjct: 1551 LDEFNELA 1558
>XP_006714963
          Length = 552

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   VEFNELA 8
           VEFN+LA
Sbjct: 295 VEFNQLA 301
>XP_006714962
          Length = 557

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   VEFNELA 8
           VEFN+LA
Sbjct: 300 VEFNQLA 306
>XP_005266010
          Length = 557

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   VEFNELA 8
           VEFN+LA
Sbjct: 300 VEFNQLA 306
>NP_115543
          Length = 813

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   LVEFNE 6
           L+EFNE
Sbjct: 602 LIEFNE 607
>XP_006721041
          Length = 680

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   LVEFNE 6
           L+EFNE
Sbjct: 469 LIEFNE 474
>XP_006721040
          Length = 661

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   LVEFNE 6
           L+EFNE
Sbjct: 450 LIEFNE 455
>XP_005255739
          Length = 823

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   LVEFNE 6
           L+EFNE
Sbjct: 612 LIEFNE 617
>NP_001138508
          Length = 737

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   EFNELAF 9
           EFN+L F
Sbjct: 176 EFNQLQF 182
>NP_031383
          Length = 738

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   EFNELAF 9
           EFN+L F
Sbjct: 176 EFNQLQF 182
>NP_542376
          Length = 1899

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2    VEFNELA 8
            V++NELA
Sbjct: 1299 VDYNELA 1305
>NP_001035502
          Length = 1031

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   VEFNELAF 9
           V FN+LAF
Sbjct: 180 VFFNKLAF 187
>NP_001035501
          Length = 1006

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   VEFNELAF 9
           V FN+LAF
Sbjct: 155 VFFNKLAF 162
>NP_004361
          Length = 3063

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2    VEFNELA 8
            V++NELA
Sbjct: 2463 VDYNELA 2469
>NP_976044
          Length = 1079

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   VEFNELAF 9
           V FN+LAF
Sbjct: 228 VFFNKLAF 235
>NP_001274320
          Length = 1006

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   VEFNELAF 9
           V FN+LAF
Sbjct: 155 VFFNKLAF 162
>NP_001035500
          Length = 1044

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   VEFNELAF 9
           V FN+LAF
Sbjct: 193 VFFNKLAF 200
>NP_002310
          Length = 683

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   EFNELAF 9
           EF+EL+F
Sbjct: 320 EFSELSF 326
>NP_001276863
          Length = 652

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   EFNELAF 9
           EF+EL+F
Sbjct: 320 EFSELSF 326
>NP_001008395
          Length = 333

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   FNELAF 9
           FN+LAF
Sbjct: 114 FNQLAF 119
>XP_005250404
          Length = 621

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   EFNELAF 9
           EF+EL+F
Sbjct: 320 EFSELSF 326
>XP_005250403
          Length = 644

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   EFNELAF 9
           EF+EL+F
Sbjct: 320 EFSELSF 326
>XP_006715397
          Length = 2972

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2    VEFNELA 8
            V++NELA
Sbjct: 2372 VDYNELA 2378
>NP_001135389
          Length = 1335

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   FNELAF 9
           FNEL+F
Sbjct: 471 FNELSF 476
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.333    0.149    0.404 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 447,628
Number of extensions: 476
Number of successful extensions: 54
Number of sequences better than 10000.0: 54
Number of HSP's gapped: 54
Number of HSP's successfully gapped: 54
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.2 bits)
S2: 28 (15.4 bits)

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