Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_000312                                                              20     509
NP_001156602                                                           17    3301
NP_001030008                                                           17    3301
NP_663629                                                              17    3301
NP_001156593                                                           17    3301
NP_001156594                                                           17    3301
NP_001161067                                                           17    4312
NP_055139                                                              17    4312
NP_005602                                                              16    5631
NP_148936                                                              16    5631
NP_001245390                                                           16    5631
NP_006703                                                              16    5631
XP_005256140                                                           16    5631
XP_005249990                                                           16    5631
XP_005249989                                                           16    5631
NP_062553                                                              16    7355
NP_899662                                                              16    7355
NP_002886                                                              16    7355
NP_060625                                                              16    7355
XP_006723905                                                           16    7355
NP_001098117                                                           16    7355
NP_861420                                                              15    9605
NP_005516                                                              15    9605
NP_001193670                                                           15    9605
NP_001257903                                                           15    9605
NP_689777                                                              15    9605
NP_055919                                                              15    9605
NP_060360                                                              15    9605
NP_001171652                                                           15    9605
NP_056058                                                              15    9605
NP_620141                                                              15    9605
XP_006724219                                                           15    9605
XP_006724218                                                           15    9605
XP_006724217                                                           15    9605
XP_006721269                                                           15    9605
XP_005273907                                                           15    9605
XP_005273906                                                           15    9605
XP_006716574                                                           15    9605
XP_006716573                                                           15    9605
XP_005250858                                                           15    9605
XP_005250857                                                           15    9605
XP_005270933                                                           15    9605
NP_071441                                                              15    9605
NP_689726                                                              15    9605
>NP_000312
          Length = 928

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   LVCALEVVM 9
           L CALEVVM
Sbjct: 487 LACALEVVM 495
>NP_001156602
          Length = 261

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   CALEVVM 9
           CALEVV+
Sbjct: 138 CALEVVL 144
>NP_001030008
          Length = 236

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   CALEVVM 9
           CALEVV+
Sbjct: 161 CALEVVL 167
>NP_663629
          Length = 284

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   CALEVVM 9
           CALEVV+
Sbjct: 161 CALEVVL 167
>NP_001156593
          Length = 251

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   CALEVVM 9
           CALEVV+
Sbjct: 161 CALEVVL 167
>NP_001156594
          Length = 228

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   CALEVVM 9
           CALEVV+
Sbjct: 138 CALEVVL 144
>NP_001161067
          Length = 394

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   LVCALEVVM 9
           L+CAL ++M
Sbjct: 158 LMCALGIIM 166
>NP_055139
          Length = 382

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   LVCALEVVM 9
           L+CAL ++M
Sbjct: 158 LMCALGIIM 166
>NP_005602
          Length = 1139

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1   LVCALEVV 8
           L C LEVV
Sbjct: 533 LACCLEVV 540
>NP_148936
          Length = 408

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1  LVCALE 6
          LVCALE
Sbjct: 35 LVCALE 40
>NP_001245390
          Length = 522

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   LVCALE 6
           LVCALE
Sbjct: 176 LVCALE 181
>NP_006703
          Length = 549

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   LVCALE 6
           LVCALE
Sbjct: 176 LVCALE 181
>XP_005256140
          Length = 1065

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1   LVCALEVV 8
           L C LEVV
Sbjct: 459 LACCLEVV 466
>XP_005249990
          Length = 515

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   LVCALE 6
           LVCALE
Sbjct: 142 LVCALE 147
>XP_005249989
          Length = 515

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   LVCALE 6
           LVCALE
Sbjct: 142 LVCALE 147
>NP_062553
          Length = 159

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1  LVCALE 6
          L+CALE
Sbjct: 93 LICALE 98
>NP_899662
          Length = 1014

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/9 (44%), Positives = 7/9 (77%)

Query: 1   LVCALEVVM 9
           + C LE+V+
Sbjct: 502 MACCLEIVL 510
>NP_002886
          Length = 1068

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/9 (44%), Positives = 7/9 (77%)

Query: 1   LVCALEVVM 9
           + C LE+V+
Sbjct: 502 MACCLEIVL 510
>NP_060625
          Length = 310

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1  LVCALEVV 8
          L C LEVV
Sbjct: 23 LTCPLEVV 30
>XP_006723905
          Length = 896

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/9 (44%), Positives = 7/9 (77%)

Query: 1   LVCALEVVM 9
           + C LE+V+
Sbjct: 330 MACCLEIVL 338
>NP_001098117
          Length = 311

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1  LVCALEVV 8
          L C LEVV
Sbjct: 23 LTCPLEVV 30
>NP_861420
          Length = 292

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/6 (66%), Positives = 6/6 (100%)

Query: 3   CALEVV 8
           CALE++
Sbjct: 241 CALEII 246
>NP_005516
          Length = 292

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/6 (66%), Positives = 6/6 (100%)

Query: 3   CALEVV 8
           CALE++
Sbjct: 241 CALEII 246
>NP_001193670
          Length = 292

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/6 (66%), Positives = 6/6 (100%)

Query: 3   CALEVV 8
           CALE++
Sbjct: 241 CALEII 246
>NP_001257903
          Length = 5121

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2    VCALEVV 8
            VC LEV+
Sbjct: 4484 VCKLEVI 4490
>NP_689777
          Length = 3997

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   LVCALE 6
           +VCALE
Sbjct: 598 IVCALE 603
>NP_055919
          Length = 1938

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 3   CALEVVM 9
           C+LEV++
Sbjct: 811 CSLEVIL 817
>NP_060360
          Length = 4022

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   LVCALE 6
           +VCALE
Sbjct: 598 IVCALE 603
>NP_001171652
          Length = 201

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 7/9 (77%)

Query: 1   LVCALEVVM 9
           ++C + VVM
Sbjct: 174 IICLIRVVM 182
>NP_056058
          Length = 863

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   LVCALE 6
           +VCALE
Sbjct: 598 IVCALE 603
>NP_620141
          Length = 201

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 7/9 (77%)

Query: 1   LVCALEVVM 9
           ++C + VVM
Sbjct: 174 IICLIRVVM 182
>XP_006724219
          Length = 1157

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1  LVCALEVV 8
          L CALE++
Sbjct: 45 LACALELL 52
>XP_006724218
          Length = 1200

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1  LVCALEVV 8
          L CALE++
Sbjct: 45 LACALELL 52
>XP_006724217
          Length = 1235

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1  LVCALEVV 8
          L CALE++
Sbjct: 45 LACALELL 52
>XP_006721269
          Length = 5138

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2    VCALEVV 8
            VC LEV+
Sbjct: 4501 VCKLEVI 4507
>XP_005273907
          Length = 1930

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 3   CALEVVM 9
           C+LEV++
Sbjct: 811 CSLEVIL 817
>XP_005273906
          Length = 1932

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 3   CALEVVM 9
           C+LEV++
Sbjct: 811 CSLEVIL 817
>XP_006716574
          Length = 968

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   LVCALE 6
           +VCALE
Sbjct: 598 IVCALE 603
>XP_006716573
          Length = 1776

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   LVCALE 6
           +VCALE
Sbjct: 598 IVCALE 603
>XP_005250858
          Length = 4022

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   LVCALE 6
           +VCALE
Sbjct: 598 IVCALE 603
>XP_005250857
          Length = 4022

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   LVCALE 6
           +VCALE
Sbjct: 598 IVCALE 603
>XP_005270933
          Length = 122

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1  LVCALEV 7
          L+C+LEV
Sbjct: 11 LMCSLEV 17
>NP_071441
          Length = 309

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 1   LVCALEVV 8
           L C LE+V
Sbjct: 203 LFCGLEIV 210
>NP_689726
          Length = 1274

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1  LVCALEVV 8
          L CALE++
Sbjct: 45 LACALELL 52
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.343    0.145    0.425 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 561,780
Number of extensions: 416
Number of successful extensions: 44
Number of sequences better than 10000.0: 44
Number of HSP's gapped: 44
Number of HSP's successfully gapped: 44
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.6 bits)
S2: 28 (15.4 bits)

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