Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_000042                                                              19     869
XP_006718907                                                           19     869
XP_006718906                                                           19     869
XP_005271619                                                           19     869
XP_005271618                                                           19     869
NP_149055                                                              17    2528
XP_005246793                                                           17    2528
XP_005246792                                                           17    2528
XP_005246791                                                           17    2528
XP_005246790                                                           17    2528
XP_005246789                                                           17    2528
NP_055109                                                              16    7355
NP_055907                                                              16    7355
NP_689615                                                              16    7355
XP_005255242                                                           16    7355
XP_005255241                                                           16    7355
XP_005255240                                                           16    7355
XP_005255239                                                           16    7355
NP_057675                                                              15    9605
NP_003679                                                              15    9605
NP_001119526                                                           15    9605
NP_001119527                                                           15    9605
NP_055132                                                              15    9605
NP_065876                                                              15    9605
XP_006724629                                                           15    9605
XP_006724628                                                           15    9605
XP_005272743                                                           15    9605
XP_006711671                                                           15    9605
XP_006711670                                                           15    9605
XP_005245629                                                           15    9605
>NP_000042
          Length = 3056

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1   LVARIIHA 8
           LVARIIHA
Sbjct: 186 LVARIIHA 193
>XP_006718907
          Length = 3050

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1   LVARIIHA 8
           LVARIIHA
Sbjct: 186 LVARIIHA 193
>XP_006718906
          Length = 3056

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1   LVARIIHA 8
           LVARIIHA
Sbjct: 186 LVARIIHA 193
>XP_005271619
          Length = 3056

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1   LVARIIHA 8
           LVARIIHA
Sbjct: 186 LVARIIHA 193
>XP_005271618
          Length = 3056

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1   LVARIIHA 8
           LVARIIHA
Sbjct: 186 LVARIIHA 193
>NP_149055
          Length = 637

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 1   LVARIIH 7
           LVARIIH
Sbjct: 178 LVARIIH 184
>XP_005246793
          Length = 562

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 1   LVARIIH 7
           LVARIIH
Sbjct: 134 LVARIIH 140
>XP_005246792
          Length = 579

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 1   LVARIIH 7
           LVARIIH
Sbjct: 151 LVARIIH 157
>XP_005246791
          Length = 593

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 1   LVARIIH 7
           LVARIIH
Sbjct: 178 LVARIIH 184
>XP_005246790
          Length = 602

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 1   LVARIIH 7
           LVARIIH
Sbjct: 134 LVARIIH 140
>XP_005246789
          Length = 613

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 1   LVARIIH 7
           LVARIIH
Sbjct: 178 LVARIIH 184
>NP_055109
          Length = 374

 Score = 15.8 bits (29), Expect =  7355
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 1  LVARIIHA 8
          LVA IIHA
Sbjct: 88 LVAIIIHA 95
>NP_055907
          Length = 3661

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2    VARIIHA 8
            VAR++HA
Sbjct: 2929 VARLLHA 2935
>NP_689615
          Length = 369

 Score = 15.8 bits (29), Expect =  7355
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 1  LVARIIHA 8
          LVA IIHA
Sbjct: 88 LVAIIIHA 95
>XP_005255242
          Length = 3609

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2    VARIIHA 8
            VAR++HA
Sbjct: 2877 VARLLHA 2883
>XP_005255241
          Length = 3637

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2    VARIIHA 8
            VAR++HA
Sbjct: 2905 VARLLHA 2911
>XP_005255240
          Length = 3663

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2    VARIIHA 8
            VAR++HA
Sbjct: 2931 VARLLHA 2937
>XP_005255239
          Length = 3689

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2    VARIIHA 8
            VAR++HA
Sbjct: 2957 VARLLHA 2963
>NP_057675
          Length = 453

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   LVARIIHA 8
           +V R+IHA
Sbjct: 145 MVERLIHA 152
>NP_003679
          Length = 921

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   LVARIIH 7
           +VARI+H
Sbjct: 514 IVARIMH 520
>NP_001119526
          Length = 898

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   LVARIIH 7
           +VARI+H
Sbjct: 514 IVARIMH 520
>NP_001119527
          Length = 897

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   LVARIIH 7
           +VARI+H
Sbjct: 508 IVARIMH 514
>NP_055132
          Length = 415

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   LVARIIHAA 9
           L+A +IH A
Sbjct: 167 LIAEMIHTA 175
>NP_065876
          Length = 1269

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 2   VARIIHA 8
           VAR++H+
Sbjct: 412 VARVVHS 418
>XP_006724629
          Length = 885

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   LVARIIH 7
           +VARI+H
Sbjct: 508 IVARIMH 514
>XP_006724628
          Length = 908

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   LVARIIH 7
           +VARI+H
Sbjct: 508 IVARIMH 514
>XP_005272743
          Length = 920

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   LVARIIH 7
           +VARI+H
Sbjct: 508 IVARIMH 514
>XP_006711671
          Length = 2153

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   ARIIHA 8
           ARI+HA
Sbjct: 277 ARIVHA 282
>XP_006711670
          Length = 2161

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   ARIIHA 8
           ARI+HA
Sbjct: 277 ARIVHA 282
>XP_005245629
          Length = 2164

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   ARIIHA 8
           ARI+HA
Sbjct: 277 ARIVHA 282
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.345    0.143    0.387 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 442,853
Number of extensions: 552
Number of successful extensions: 30
Number of sequences better than 10000.0: 30
Number of HSP's gapped: 30
Number of HSP's successfully gapped: 30
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.5 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.8 bits)
S2: 28 (15.4 bits)

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