Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_001136098                                                           18    1482
NP_000360                                                              18    1482
NP_001018046                                                           18    1482
XP_005268096                                                           18    1482
XP_006720308                                                           18    1482
XP_005268094                                                           18    1482
NP_003242                                                              17    2528
NP_036277                                                              17    3301
NP_001158503                                                           17    3301
NP_085046                                                              17    4312
NP_001001851                                                           17    4312
NP_055423                                                              16    5631
NP_001274739                                                           16    5631
NP_001028200                                                           16    5631
XP_005272600                                                           16    5631
NP_001005197                                                           16    5631
NP_056224                                                              16    7355
NP_001191236                                                           16    7355
NP_001191235                                                           16    7355
NP_000574                                                              16    7355
NP_001138624                                                           16    7355
NP_689628                                                              16    7355
NP_001186306                                                           16    7355
NP_055977                                                              16    7355
XP_005273458                                                           16    7355
XP_005273457                                                           16    7355
XP_006714240                                                           16    7355
NP_057700                                                              15    9605
NP_060218                                                              15    9605
NP_004722                                                              15    9605
NP_001257552                                                           15    9605
NP_660333                                                              15    9605
NP_001257551                                                           15    9605
NP_003694                                                              15    9605
NP_982278                                                              15    9605
XP_006722363                                                           15    9605
XP_006719756                                                           15    9605
XP_005269288                                                           15    9605
XP_006713992                                                           15    9605
NP_001020471                                                           15    9605
>NP_001136098
          Length = 274

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   LTVIDKYAF 9
           LTVIDK AF
Sbjct: 213 LTVIDKDAF 221
>NP_000360
          Length = 764

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   LTVIDKYAF 9
           LTVIDK AF
Sbjct: 213 LTVIDKDAF 221
>NP_001018046
          Length = 253

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   LTVIDKYAF 9
           LTVIDK AF
Sbjct: 213 LTVIDKDAF 221
>XP_005268096
          Length = 253

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   LTVIDKYAF 9
           LTVIDK AF
Sbjct: 213 LTVIDKDAF 221
>XP_006720308
          Length = 274

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   LTVIDKYAF 9
           LTVIDK AF
Sbjct: 213 LTVIDKDAF 221
>XP_005268094
          Length = 764

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   LTVIDKYAF 9
           LTVIDK AF
Sbjct: 213 LTVIDKDAF 221
>NP_003242
          Length = 146

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1  LTVIDKYA 8
          LTV+D+YA
Sbjct: 14 LTVMDRYA 21
>NP_036277
          Length = 212

 Score = 16.9 bits (32), Expect =  3301
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   VIDKYAF 9
           V+D+YAF
Sbjct: 94  VVDRYAF 100
>NP_001158503
          Length = 188

 Score = 16.9 bits (32), Expect =  3301
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   VIDKYAF 9
           V+D+YAF
Sbjct: 94  VVDRYAF 100
>NP_085046
          Length = 942

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2  TVIDKYAF 9
          T+I +YAF
Sbjct: 59 TIISRYAF 66
>NP_001001851
          Length = 702

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2  TVIDKYAF 9
          T+I +YAF
Sbjct: 59 TIISRYAF 66
>NP_055423
          Length = 1253

 Score = 16.2 bits (30), Expect =  5631
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 1    LTVIDKY 7
            +T++DKY
Sbjct: 1219 ITILDKY 1225
>NP_001274739
          Length = 1253

 Score = 16.2 bits (30), Expect =  5631
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 1    LTVIDKY 7
            +T++DKY
Sbjct: 1219 ITILDKY 1225
>NP_001028200
          Length = 822

 Score = 16.2 bits (30), Expect =  5631
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 1   LTVIDKY 7
           +T++DKY
Sbjct: 788 ITILDKY 794
>XP_005272600
          Length = 1253

 Score = 16.2 bits (30), Expect =  5631
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 1    LTVIDKY 7
            +T++DKY
Sbjct: 1219 ITILDKY 1225
>NP_001005197
          Length = 314

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   LTVIDKYAF 9
           LT+I  YAF
Sbjct: 212 LTIIISYAF 220
>NP_056224
          Length = 3123

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    LTVIDKYA 8
            LT I+KYA
Sbjct: 2123 LTPIEKYA 2130
>NP_001191236
          Length = 493

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   VIDKYAF 9
           V+DKY F
Sbjct: 361 VMDKYTF 367
>NP_001191235
          Length = 474

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   VIDKYAF 9
           V+DKY F
Sbjct: 342 VMDKYTF 348
>NP_000574
          Length = 474

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   VIDKYAF 9
           V+DKY F
Sbjct: 342 VMDKYTF 348
>NP_001138624
          Length = 372

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   TVIDKY 7
           TV+DKY
Sbjct: 271 TVLDKY 276
>NP_689628
          Length = 397

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   TVIDKY 7
           TV+DKY
Sbjct: 296 TVLDKY 301
>NP_001186306
          Length = 720

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   LTVIDKY 7
           LTV++KY
Sbjct: 701 LTVLEKY 707
>NP_055977
          Length = 724

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   LTVIDKY 7
           LTV++KY
Sbjct: 705 LTVLEKY 711
>XP_005273458
          Length = 307

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   TVIDKY 7
           TV+DKY
Sbjct: 206 TVLDKY 211
>XP_005273457
          Length = 307

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   TVIDKY 7
           TV+DKY
Sbjct: 206 TVLDKY 211
>XP_006714240
          Length = 425

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   VIDKYAF 9
           V+DKY F
Sbjct: 342 VMDKYTF 348
>NP_057700
          Length = 588

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1  LTVIDKY 7
          LTVID Y
Sbjct: 29 LTVIDVY 35
>NP_060218
          Length = 425

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 5/6 (83%)

Query: 4  IDKYAF 9
          IDKY F
Sbjct: 92 IDKYVF 97
>NP_004722
          Length = 478

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   LTVIDKYAF 9
           LT+I KY +
Sbjct: 132 LTIIGKYFY 140
>NP_001257552
          Length = 478

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   LTVIDKYAF 9
           LT+I KY +
Sbjct: 132 LTIIGKYFY 140
>NP_660333
          Length = 1321

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1    LTVIDKYAF 9
            LTV+ +YA+
Sbjct: 1184 LTVLREYAY 1192
>NP_001257551
          Length = 478

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   LTVIDKYAF 9
           LT+I KY +
Sbjct: 132 LTIIGKYFY 140
>NP_003694
          Length = 857

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   LTVIDK 6
           LTVIDK
Sbjct: 492 LTVIDK 497
>NP_982278
          Length = 295

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/6 (66%), Positives = 6/6 (100%)

Query: 4   IDKYAF 9
           +DKY+F
Sbjct: 205 VDKYSF 210
>XP_006722363
          Length = 212

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   LTVIDKYA 8
           + +IDKYA
Sbjct: 105 MKMIDKYA 112
>XP_006719756
          Length = 478

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   LTVIDKYAF 9
           LT+I KY +
Sbjct: 132 LTIIGKYFY 140
>XP_005269288
          Length = 478

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   LTVIDKYAF 9
           LT+I KY +
Sbjct: 132 LTIIGKYFY 140
>XP_006713992
          Length = 857

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   LTVIDK 6
           LTVIDK
Sbjct: 492 LTVIDK 497
>NP_001020471
          Length = 244

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   LTVIDKYA 8
           + +IDKYA
Sbjct: 137 MKMIDKYA 144
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.335    0.149    0.424 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 471,138
Number of extensions: 757
Number of successful extensions: 40
Number of sequences better than 10000.0: 40
Number of HSP's gapped: 40
Number of HSP's successfully gapped: 40
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.3 bits)
S2: 28 (15.4 bits)

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