Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_001013433                                                           19     665
NP_060785                                                              19     665
NP_361014                                                              19     665
NP_001257903                                                           17    4312
NP_001307                                                              17    4312
XP_006721269                                                           17    4312
NP_115642                                                              16    5631
NP_005086                                                              16    5631
XP_005259360                                                           16    5631
XP_005271388                                                           16    5631
XP_006711098                                                           16    5631
XP_006711097                                                           16    5631
XP_005271387                                                           16    5631
XP_005271385                                                           16    5631
NP_001123483                                                           16    5631
NP_113639                                                              16    5631
NP_001073936                                                           15    9605
NP_071750                                                              15    9605
NP_945314                                                              15    9605
XP_005256148                                                           15    9605
XP_006721307                                                           15    9605
XP_005256144                                                           15    9605
XP_005256143                                                           15    9605
XP_005256142                                                           15    9605
>NP_001013433
          Length = 589

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1   LTSGMELK 8
           LTSGMELK
Sbjct: 465 LTSGMELK 472
>NP_060785
          Length = 627

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1   LTSGMELK 8
           LTSGMELK
Sbjct: 503 LTSGMELK 510
>NP_361014
          Length = 707

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1   LTSGMELK 8
           LTSGMELK
Sbjct: 583 LTSGMELK 590
>NP_001257903
          Length = 5121

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1    LTSGMELKY 9
            +TSGME+ +
Sbjct: 4581 ITSGMEVSF 4589
>NP_001307
          Length = 971

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   LTSGMELKY 9
           +T+G E+KY
Sbjct: 302 VTTGQEVKY 310
>XP_006721269
          Length = 5138

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1    LTSGMELKY 9
            +TSGME+ +
Sbjct: 4598 ITSGMEVSF 4606
>NP_115642
          Length = 1984

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1    LTSGMELKY 9
            L SG+ LKY
Sbjct: 1110 LASGLVLKY 1118
>NP_005086
          Length = 1548

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3    SGMELKY 9
            SGM LKY
Sbjct: 1201 SGMTLKY 1207
>XP_005259360
          Length = 281

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1  LTSGMEL 7
          LTSG+EL
Sbjct: 52 LTSGLEL 58
>XP_005271388
          Length = 1224

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   SGMELKY 9
           SGM LKY
Sbjct: 877 SGMTLKY 883
>XP_006711098
          Length = 1516

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3    SGMELKY 9
            SGM LKY
Sbjct: 1169 SGMTLKY 1175
>XP_006711097
          Length = 1542

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3    SGMELKY 9
            SGM LKY
Sbjct: 1195 SGMTLKY 1201
>XP_005271387
          Length = 1545

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3    SGMELKY 9
            SGM LKY
Sbjct: 1198 SGMTLKY 1204
>XP_005271385
          Length = 1551

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3    SGMELKY 9
            SGM LKY
Sbjct: 1204 SGMTLKY 1210
>NP_001123483
          Length = 249

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1  LTSGMEL 7
          LTSG+EL
Sbjct: 20 LTSGLEL 26
>NP_113639
          Length = 267

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1  LTSGMEL 7
          LTSG+EL
Sbjct: 38 LTSGLEL 44
>NP_001073936
          Length = 1848

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 2    TSGMELKY 9
            ++GM+L+Y
Sbjct: 1707 STGMQLRY 1714
>NP_071750
          Length = 486

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   LTSGMEL 7
           LTSG EL
Sbjct: 439 LTSGQEL 445
>NP_945314
          Length = 1386

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   TSGMELKY 9
           T G+EL+Y
Sbjct: 273 TIGLELEY 280
>XP_005256148
          Length = 278

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   LTSGMEL 7
           LTSG EL
Sbjct: 231 LTSGQEL 237
>XP_006721307
          Length = 370

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   LTSGMEL 7
           LTSG EL
Sbjct: 323 LTSGQEL 329
>XP_005256144
          Length = 486

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   LTSGMEL 7
           LTSG EL
Sbjct: 439 LTSGQEL 445
>XP_005256143
          Length = 486

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   LTSGMEL 7
           LTSG EL
Sbjct: 439 LTSGQEL 445
>XP_005256142
          Length = 486

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   LTSGMEL 7
           LTSG EL
Sbjct: 439 LTSGQEL 445
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.312    0.131    0.344 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 518,677
Number of extensions: 819
Number of successful extensions: 24
Number of sequences better than 10000.0: 24
Number of HSP's gapped: 24
Number of HSP's successfully gapped: 24
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.5 bits)
S2: 28 (15.4 bits)

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