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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_940873                                                              16    5631
XP_006722781                                                           16    5631
XP_005272524                                                           16    5631
NP_001104251                                                           16    7355
NP_001277193                                                           16    7355
XP_006710934                                                           16    7355
XP_006710933                                                           16    7355
NP_000575                                                              15    9605
NP_714544                                                              15    9605
XP_006720470                                                           15    9605

>NP_940873
          Length = 821

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2   TSALAVAM 9
           TSALA+A+
Sbjct: 764 TSALAIAL 771



>XP_006722781
          Length = 821

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2   TSALAVAM 9
           TSALA+A+
Sbjct: 764 TSALAIAL 771



>XP_005272524
          Length = 872

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2   TSALAVAM 9
           TSALA+A+
Sbjct: 815 TSALAIAL 822



>NP_001104251
          Length = 405

 Score = 15.8 bits (29), Expect =  7355
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2   TSALAVAM 9
           TSA AVAM
Sbjct: 262 TSAAAVAM 269



>NP_001277193
          Length = 405

 Score = 15.8 bits (29), Expect =  7355
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2   TSALAVAM 9
           TSA AVAM
Sbjct: 262 TSAAAVAM 269



>XP_006710934
          Length = 465

 Score = 15.8 bits (29), Expect =  7355
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2   TSALAVAM 9
           TSA AVAM
Sbjct: 368 TSAAAVAM 375



>XP_006710933
          Length = 511

 Score = 15.8 bits (29), Expect =  7355
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2   TSALAVAM 9
           TSA AVAM
Sbjct: 368 TSAAAVAM 375



>NP_000575
          Length = 99

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1 LTSALAVAM 9
         +TS LAVA+
Sbjct: 1 MTSKLAVAL 9



>NP_714544
          Length = 1775

 Score = 15.4 bits (28), Expect =  9605
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1    LTSALAVAM 9
            LTSA AVA+
Sbjct: 1708 LTSAQAVAV 1716



>XP_006720470
          Length = 1017

 Score = 15.4 bits (28), Expect =  9605
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   LTSALAVAM 9
           LTSA AVA+
Sbjct: 950 LTSAQAVAV 958



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.324    0.117    0.260 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 164,983
Number of extensions: 88
Number of successful extensions: 10
Number of sequences better than 10000.0: 10
Number of HSP's gapped: 10
Number of HSP's successfully gapped: 10
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.2 bits)
S2: 28 (15.4 bits)