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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

XP_005270532                                                           17    2528
NP_777556                                                              17    2528
NP_000360                                                              17    3301
XP_005268094                                                           17    3301
NP_003205                                                              17    4312
NP_001170946                                                           16    5631
NP_689794                                                              16    5631
NP_689418                                                              16    7355

>XP_005270532
          Length = 130

 Score = 17.3 bits (33), Expect =  2528
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1  LTLIIVAFV 9
          LTL+++AF+
Sbjct: 50 LTLLLIAFI 58



>NP_777556
          Length = 155

 Score = 17.3 bits (33), Expect =  2528
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1  LTLIIVAFV 9
          LTL+++AF+
Sbjct: 50 LTLLLIAFI 58



>NP_000360
          Length = 764

 Score = 16.9 bits (32), Expect =  3301
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   LTLIIVAFV 9
           LTL IVAFV
Sbjct: 587 LTLNIVAFV 595



>XP_005268094
          Length = 764

 Score = 16.9 bits (32), Expect =  3301
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   LTLIIVAFV 9
           LTL IVAFV
Sbjct: 587 LTLNIVAFV 595



>NP_003205
          Length = 435

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   TLIIVAFV 9
           TL+++AFV
Sbjct: 408 TLLVIAFV 415



>NP_001170946
          Length = 455

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1   LTLIIVAFV 9
           LT++++AFV
Sbjct: 438 LTMLLLAFV 446



>NP_689794
          Length = 518

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1   LTLIIVAFV 9
           LT++++AFV
Sbjct: 501 LTMLLLAFV 509



>NP_689418
          Length = 839

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1   LTLIIVAFV 9
           LTL++VA++
Sbjct: 608 LTLLLVAYM 616



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.356    0.166    0.419 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 251,062
Number of extensions: 249
Number of successful extensions: 8
Number of sequences better than 10000.0: 8
Number of HSP's gapped: 8
Number of HSP's successfully gapped: 8
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 14 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (17.0 bits)
S2: 28 (15.4 bits)