Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_776173                                                              17    4312
XP_005276340                                                           17    4312
XP_006719419                                                           17    4312
XP_006719416                                                           17    4312
XP_006719415                                                           17    4312
XP_006719414                                                           17    4312
XP_006719412                                                           17    4312
XP_006719411                                                           17    4312
XP_005249981                                                           17    4312
NP_001191746                                                           16    5631
NP_116217                                                              16    5631
NP_689688                                                              16    5631
XP_006723092                                                           16    5631
NP_060145                                                              16    7355
NP_001191385                                                           16    7355
NP_001165447                                                           16    7355
NP_004710                                                              16    7355
XP_006719755                                                           16    7355
XP_005269287                                                           16    7355
XP_006714950                                                           16    7355
NP_066986                                                              16    7355
NP_001007189                                                           15    9605
NP_001229825                                                           15    9605
NP_001229826                                                           15    9605
NP_055477                                                              15    9605
NP_001129501                                                           15    9605
NP_001273206                                                           15    9605
NP_001273207                                                           15    9605
NP_001273208                                                           15    9605
NP_006313                                                              15    9605
XP_005261924                                                           15    9605
XP_006724450                                                           15    9605
XP_006724449                                                           15    9605
XP_005261923                                                           15    9605
XP_006724448                                                           15    9605
XP_006724447                                                           15    9605
XP_005261922                                                           15    9605
XP_005261921                                                           15    9605
XP_005261920                                                           15    9605
XP_005261919                                                           15    9605
XP_005268350                                                           15    9605
>NP_776173
          Length = 758

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2   SYSRKSAQ 9
           +YSRKSA+
Sbjct: 676 TYSRKSAK 683
>XP_005276340
          Length = 158

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2  SYSRKSAQ 9
          +YSRKSA+
Sbjct: 86 TYSRKSAK 93
>XP_006719419
          Length = 443

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2   SYSRKSAQ 9
           +YSRKSA+
Sbjct: 361 TYSRKSAK 368
>XP_006719416
          Length = 615

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2   SYSRKSAQ 9
           +YSRKSA+
Sbjct: 533 TYSRKSAK 540
>XP_006719415
          Length = 615

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2   SYSRKSAQ 9
           +YSRKSA+
Sbjct: 533 TYSRKSAK 540
>XP_006719414
          Length = 708

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2   SYSRKSAQ 9
           +YSRKSA+
Sbjct: 626 TYSRKSAK 633
>XP_006719412
          Length = 724

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2   SYSRKSAQ 9
           +YSRKSA+
Sbjct: 676 TYSRKSAK 683
>XP_006719411
          Length = 738

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2   SYSRKSAQ 9
           +YSRKSA+
Sbjct: 656 TYSRKSAK 663
>XP_005249981
          Length = 158

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2  SYSRKSAQ 9
          +YSRKSA+
Sbjct: 86 TYSRKSAK 93
>NP_001191746
          Length = 574

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   SYSRKSA 8
           SYSRKS+
Sbjct: 275 SYSRKSS 281
>NP_116217
          Length = 575

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   SYSRKSA 8
           SYSRKS+
Sbjct: 276 SYSRKSS 282
>NP_689688
          Length = 575

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   SYSRKSA 8
           SYSRKS+
Sbjct: 276 SYSRKSS 282
>XP_006723092
          Length = 574

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   SYSRKSA 8
           SYSRKS+
Sbjct: 275 SYSRKSS 281
>NP_060145
          Length = 1310

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   LSYSRKSA 8
           LSY+ KSA
Sbjct: 191 LSYNNKSA 198
>NP_001191385
          Length = 714

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   SYSRKSA 8
           S+SRKSA
Sbjct: 381 SFSRKSA 387
>NP_001165447
          Length = 1310

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   LSYSRKSA 8
           LSY+ KSA
Sbjct: 191 LSYNNKSA 198
>NP_004710
          Length = 1463

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2    SYSRKSAQ 9
            SY RKS Q
Sbjct: 1122 SYKRKSEQ 1129
>XP_006719755
          Length = 1418

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2    SYSRKSAQ 9
            SY RKS Q
Sbjct: 1077 SYKRKSEQ 1084
>XP_005269287
          Length = 1527

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2    SYSRKSAQ 9
            SY RKS Q
Sbjct: 1186 SYKRKSEQ 1193
>XP_006714950
          Length = 1304

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   LSYSRKSA 8
           LSY+ KSA
Sbjct: 191 LSYNNKSA 198
>NP_066986
          Length = 714

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   SYSRKSA 8
           S+SRKSA
Sbjct: 381 SFSRKSA 387
>NP_001007189
          Length = 559

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   SYSRKSA 8
           S+SRKSA
Sbjct: 489 SHSRKSA 495
>NP_001229825
          Length = 1603

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   SYSRKSA 8
           S+SRKSA
Sbjct: 489 SHSRKSA 495
>NP_001229826
          Length = 1503

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   SYSRKSA 8
           S+SRKSA
Sbjct: 489 SHSRKSA 495
>NP_055477
          Length = 1572

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   SYSRKSA 8
           S+SRKSA
Sbjct: 489 SHSRKSA 495
>NP_001129501
          Length = 1594

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   SYSRKSA 8
           S+SRKSA
Sbjct: 489 SHSRKSA 495
>NP_001273206
          Length = 897

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   LSYSRKSAQ 9
           LSY  +SAQ
Sbjct: 131 LSYQDRSAQ 139
>NP_001273207
          Length = 824

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   LSYSRKSAQ 9
           LSY  +SAQ
Sbjct: 141 LSYQDRSAQ 149
>NP_001273208
          Length = 439

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   LSYSRKSAQ 9
           LSY  +SAQ
Sbjct: 141 LSYQDRSAQ 149
>NP_006313
          Length = 907

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   LSYSRKSAQ 9
           LSY  +SAQ
Sbjct: 141 LSYQDRSAQ 149
>XP_005261924
          Length = 1176

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   SYSRKSA 8
           S+SRKSA
Sbjct: 489 SHSRKSA 495
>XP_006724450
          Length = 1187

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   SYSRKSA 8
           S+SRKSA
Sbjct: 489 SHSRKSA 495
>XP_006724449
          Length = 1198

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   SYSRKSA 8
           S+SRKSA
Sbjct: 489 SHSRKSA 495
>XP_005261923
          Length = 1307

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   SYSRKSA 8
           S+SRKSA
Sbjct: 489 SHSRKSA 495
>XP_006724448
          Length = 1490

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   SYSRKSA 8
           S+SRKSA
Sbjct: 376 SHSRKSA 382
>XP_006724447
          Length = 1516

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   SYSRKSA 8
           S+SRKSA
Sbjct: 489 SHSRKSA 495
>XP_005261922
          Length = 1525

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   SYSRKSA 8
           S+SRKSA
Sbjct: 489 SHSRKSA 495
>XP_005261921
          Length = 1581

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   SYSRKSA 8
           S+SRKSA
Sbjct: 489 SHSRKSA 495
>XP_005261920
          Length = 1594

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   SYSRKSA 8
           S+SRKSA
Sbjct: 489 SHSRKSA 495
>XP_005261919
          Length = 1603

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   SYSRKSA 8
           S+SRKSA
Sbjct: 489 SHSRKSA 495
>XP_005268350
          Length = 818

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   LSYSRKSAQ 9
           LSY  +SAQ
Sbjct: 141 LSYQDRSAQ 149
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.301    0.107    0.249 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 422,772
Number of extensions: 353
Number of successful extensions: 41
Number of sequences better than 10000.0: 41
Number of HSP's gapped: 41
Number of HSP's successfully gapped: 41
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 17 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.4 bits)
S2: 28 (15.4 bits)

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