Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_722576                                                              19     869
XP_005262424                                                           19     869
NP_055256                                                              18    1135
NP_055200                                                              18    1135
NP_001185487                                                           18    1135
XP_006723209                                                           18    1135
XP_006723203                                                           18    1135
NP_001273385                                                           17    2528
XP_005249342                                                           17    2528
NP_056297                                                              17    2528
NP_443729                                                              17    4312
NP_201586                                                              17    4312
NP_997698                                                              16    5631
NP_001597                                                              16    5631
XP_006717059                                                           16    5631
NP_976031                                                              16    7355
NP_001136272                                                           15    9605
NP_001185744                                                           15    9605
XP_005275558                                                           15    9605
XP_005275391                                                           15    9605
XP_005275259                                                           15    9605
XP_005274965                                                           15    9605
XP_005272815                                                           15    9605
XP_005274885                                                           15    9605
XP_005248836                                                           15    9605
>NP_722576
          Length = 3080

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    LSVNVTAFT 9
            L VNVTAFT
Sbjct: 1516 LPVNVTAFT 1524
>XP_005262424
          Length = 2875

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    LSVNVTAFT 9
            L VNVTAFT
Sbjct: 1311 LPVNVTAFT 1319
>NP_055256
          Length = 463

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   LSVNVTAFT 9
           LSVNVTA T
Sbjct: 135 LSVNVTALT 143
>NP_055200
          Length = 467

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   LSVNVTAFT 9
           LSVNVTA T
Sbjct: 139 LSVNVTALT 147
>NP_001185487
          Length = 479

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   LSVNVTAFT 9
           LSVNVTA T
Sbjct: 135 LSVNVTALT 143
>XP_006723209
          Length = 481

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   LSVNVTAFT 9
           LSVNVTA T
Sbjct: 135 LSVNVTALT 143
>XP_006723203
          Length = 343

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   LSVNVTAFT 9
           LSVNVTA T
Sbjct: 139 LSVNVTALT 147
>NP_001273385
          Length = 361

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   LSVNVTAFT 9
           LSVNVT F+
Sbjct: 276 LSVNVTMFS 284
>XP_005249342
          Length = 694

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   LSVNVTAFT 9
           LSVNVT F+
Sbjct: 276 LSVNVTMFS 284
>NP_056297
          Length = 686

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   LSVNVTAFT 9
           LSVNVT F+
Sbjct: 276 LSVNVTMFS 284
>NP_443729
          Length = 595

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 1   LSVNVTA 7
           LSVNVTA
Sbjct: 137 LSVNVTA 143

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   LSVNVTAFT 9
           LSV+VTA T
Sbjct: 264 LSVHVTALT 272
>NP_201586
          Length = 477

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   LSVNVTAFT 9
           LSV+VTA T
Sbjct: 146 LSVHVTALT 154
>NP_997698
          Length = 2466

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   LSVNVTAFT 9
           LS++VTA T
Sbjct: 791 LSISVTALT 799
>NP_001597
          Length = 2436

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   LSVNVTAFT 9
           LS++VTA T
Sbjct: 761 LSISVTALT 769
>XP_006717059
          Length = 2435

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   LSVNVTAFT 9
           LS++VTA T
Sbjct: 760 LSISVTALT 768
>NP_976031
          Length = 352

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   LSVNVTAF 8
           + VN+TAF
Sbjct: 329 VEVNITAF 336
>NP_001136272
          Length = 3144

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2    SVNVTAFT 9
            S+N  AFT
Sbjct: 2230 SINTNAFT 2237
>NP_001185744
          Length = 1773

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2  SVNVTAFT 9
          S N TAFT
Sbjct: 25 SENTTAFT 32
>XP_005275558
          Length = 1776

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2  SVNVTAFT 9
          S N TAFT
Sbjct: 28 SENTTAFT 35
>XP_005275391
          Length = 1776

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2  SVNVTAFT 9
          S N TAFT
Sbjct: 28 SENTTAFT 35
>XP_005275259
          Length = 1776

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2  SVNVTAFT 9
          S N TAFT
Sbjct: 28 SENTTAFT 35
>XP_005274965
          Length = 1776

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2  SVNVTAFT 9
          S N TAFT
Sbjct: 28 SENTTAFT 35
>XP_005272815
          Length = 1776

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2  SVNVTAFT 9
          S N TAFT
Sbjct: 28 SENTTAFT 35
>XP_005274885
          Length = 1776

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2  SVNVTAFT 9
          S N TAFT
Sbjct: 28 SENTTAFT 35
>XP_005248836
          Length = 1776

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2  SVNVTAFT 9
          S N TAFT
Sbjct: 28 SENTTAFT 35
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.322    0.124    0.314 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 284,197
Number of extensions: 363
Number of successful extensions: 26
Number of sequences better than 10000.0: 25
Number of HSP's gapped: 26
Number of HSP's successfully gapped: 26
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.0 bits)
S2: 28 (15.4 bits)

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